LOCUS       sequence-43283-e       13853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 13853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5
FEATURES             Location/Qualifiers
     source          1..13853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..37
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21..37
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        97..740
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     634..657
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     776..799
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        1962..2177
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2178..2981
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     3154..3170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3187..3418
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3814..4119)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3916..3935)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4464..5123)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5038..5057
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5124..5226)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5371..5602)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7947..8193
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7962..7980)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8233..8249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8343..9137
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPE*KKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             10110..10772
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      11184..12526
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12147..12194)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12560..12578
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12616..12638)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12738..12757
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        12778..13059
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             13060..13734
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 atcaacgttg gtcattcaca caggaaacag ctatgaccat gtgacttgac tgatttcagt
       61 tcagtaggtc taaatcgtac gaatcatcgc agtttatcat tatcaatact cgccatttca
      121 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
      181 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
      241 ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag
      301 cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc
      361 ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac
      421 aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga
      481 tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc
      541 ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat
      601 tattccccta cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa
      661 atctatttct taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa
      721 acaccagaac ttagtttcga cggattctag aaaggatcct caacaaaaaa ttgttaatat
      781 acctctatac tttaacgtca aggagaaaaa actataatga ctaaatctca ttcagaagaa
      841 gtgattgtac ctgagttcaa ttctagcgca aaggaattac caagaccatt ggccgaaaag
      901 tgcggtattc caagtcttgc caagcccaga tccggagctt ggctgttgcc cgtctcactg
      961 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1021 gattcattaa tgcagctggc acgacaggtt tcccgactta atcgccttgc agcacatccc
     1081 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1141 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     1201 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     1261 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
     1321 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     1381 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
     1441 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
     1501 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg
     1561 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
     1621 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
     1681 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
     1741 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
     1801 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
     1861 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
     1921 gtggttgaac tgcacaccga gctcaacccc cataccacct tttcaattca tcattttttt
     1981 tttattcttt tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac
     2041 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
     2101 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
     2161 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
     2221 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
     2281 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     2341 tttgtttact aaaaactcat gtggacatct tgactgattt ttctatggag ggcacagtta
     2401 agccgctaaa ggcattatcc gccaagtaca attttttact ctttgaagac agaaaatttg
     2461 ctgacattgg taatacagtc aaattgcaat actctgcggg tgtttacaga atagcagaat
     2521 gggcagacat tacgaatgca cacggtgtgg tgggtccagg tattgttagc ggtttgaagc
     2581 aggcggcaga agaagtaaca aaggaaccaa ggggcctttt gatgttagca gaattgtcat
     2641 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
     2701 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
     2761 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
     2821 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
     2881 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
     2941 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
     3001 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattaccct
     3061 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     3121 gcgttaatgg gtttcatggt atcagagatc tttgtaaaac gacggccagt cttaagctcg
     3181 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa cacattgatg
     3241 agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3301 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3361 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3421 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3481 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3541 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3601 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3661 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3721 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3781 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3841 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3901 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3961 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     4021 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     4081 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     4141 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     4201 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     4261 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4321 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4381 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4441 agtaagccgg atccacgcgg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt
     4501 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     4561 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4621 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4681 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4741 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4801 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4861 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     4921 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4981 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     5041 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     5101 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     5161 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     5221 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     5281 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
     5341 gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac taataccatc taagtagttg
     5401 attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     5461 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     5521 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     5581 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtaacta
     5641 gtgctggagg gtgtaccacc aatctcgagt attcagcatg ctggtaagct agcttttcga
     5701 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     5761 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     5821 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     5881 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     5941 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     6001 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     6061 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     6121 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     6181 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     6241 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     6301 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     6361 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     6421 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     6481 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     6541 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     6601 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     6661 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     6721 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     6781 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     6841 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     6901 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     6961 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     7021 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     7081 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     7141 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
     7201 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     7261 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     7321 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     7381 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     7441 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     7501 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     7561 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     7621 gccattgtca gacatgtata ccccatacgt cttcccgagc gaaaacggtc tgcgctgcgg
     7681 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     7741 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     7801 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
     7861 cccgtcagta tcggcggaat ttcaactgag cgccggtcgc taccattacc agttggtctg
     7921 gtgtcaaaaa taatgatagt catgagcatg taattagtta tgtcacgctt acattcacgc
     7981 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     8041 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     8101 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     8161 aaggttttgg gacgctcgaa ggctttaatt tgcgggccct gtcatacatt atcaacagga
     8221 cttcataagc aaactggccg tcgttttaca tcggccgcca acagtgaatt ggagttcgtc
     8281 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
     8341 aaatggctaa aatgagaata tcaccggaat agaaaaaact gatcgaaaaa taccgctgcg
     8401 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
     8461 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     8521 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     8581 aacggcatga tggctggagc aatctgctaa tgtctgaggc cgatggagtc ctttgctcgg
     8641 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     8701 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     8761 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     8821 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     8881 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     8941 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
     9001 acattgcctt ctgcgtccgg tcgatcaggg aggacatcgg ggaagaacag tatgtcgagc
     9061 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatac tatattttac
     9121 tggatgaatt gttttagtac ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc
     9181 accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg cacactagac
     9241 tttgctcgca aagtcgtgac cgcctacggc ggctgcggca ccctacgggc ttgctctccg
     9301 ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt ggacgatggc
     9361 cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc
     9421 tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca
     9481 gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt
     9541 tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt
     9601 tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt
     9661 gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc
     9721 gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt
     9781 gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg
     9841 agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca
     9901 aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg
     9961 gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat
    10021 ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gccgcaaccg
    10081 tgcggcaccc ctaccgcatg gagataagca tggccacgca gtccagagaa atcggcattc
    10141 aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat gaggcgtggg
    10201 ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc gtggcgcaga
    10261 tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc atcggacgtt
    10321 ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg atctccgtcg
    10381 tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg
    10441 gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg
    10501 acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg accctgtatc
    10561 cgggcgagca gcaactaccg accggccccg gcgaggagcc gcccagccag cccggcattc
    10621 cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc
    10681 agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag ccgttggagc
    10741 cgccgacacg ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca acccgtaagt
    10801 gcgctgttcc agactatcgg ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc
    10861 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
    10921 tcgctaacgg attcaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt
    10981 caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca
    11041 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
    11101 tttaacgacc ctgccctgaa ccgacgaccg cgttcttcgg ggcgaaaact ctccgcgcac
    11161 atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat
    11221 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
    11281 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca
    11341 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
    11401 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
    11461 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
    11521 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
    11581 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
    11641 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
    11701 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
    11761 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
    11821 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
    11881 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
    11941 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
    12001 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
    12061 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
    12121 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
    12181 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
    12241 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
    12301 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
    12361 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
    12421 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
    12481 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca
    12541 ttattaatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    12601 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    12661 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    12721 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac
    12781 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
    12841 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
    12901 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat
    12961 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg
    13021 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca
    13081 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt
    13141 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca
    13201 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca
    13261 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct
    13321 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa
    13381 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac
    13441 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa
    13501 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa
    13561 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt
    13621 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat
    13681 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt
    13741 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata
    13801 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aat
//
