LOCUS       sequence-432834-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 BA.4
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3824..4412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3877..3897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4295..4306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4465..4586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4476..4495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4530..4549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4701..4747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4777..4913
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4958..5029
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5074..5270
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5121..5256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5284..6078
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5338..5357)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5948..5967
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6089..6313
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6509..7097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6589..6608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7843..7911,7912..8703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8061..8080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8716..8852
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9004..9075
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9080..9126)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9144..9192
                     /note="synthetic polyadenylation signal"
     enhancer        9273..9576
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9578..9780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9731..9751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9914..10389
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcataact
       61 agaacgcagt cttacacgaa ctcttttact cgtggagtat actatcccga taaagtcttt
      121 aggtccagtg tccttcattc tactcaagac cttttcctgc cattcttctc aaatgtcacc
      181 tggttccatg ctatctctgg caccaacgga actaaacgat ttgataatcc cgtcttgcca
      241 tttaacgatg gcgtatactt tgcctcaacg gagaagtcca acattatccg gggatggata
      301 tttggcacca ccctggactc caagacacag tctctcctca ttgtgaacaa cgccaccaat
      361 gtcgtcatca aagtctgtga atttcaattt tgtaacgatc cctttctcga tgtttattat
      421 cataagaata acaagtcttg gatggagtca gagttccgcg tttacagttc cgctaacaac
      481 tgtacttttg agtacgtttc ccaaccattt ctcatggatc ttgaagggaa gcaaggcaac
      541 ttcaagaatc tccgcgaatt tgtgtttaag aatatagacg gctacttcaa aatttatagc
      601 aagcataccc cgataaatct gggtcgggat ctccctcaag gcttctccgc gttggaaccc
      661 ctcgtggact tgccgattgg catcaacatc acccggttcc aaacccttct tgcccttcat
      721 cgaagctacc tcacgcctgg cgacagtagt agtggttgga cagcgggcgc agcagcctac
      781 tatgtgggat atttgcaacc acgcaccttt cttctcaaat ataacgaaaa cggaacaatt
      841 accgatgccg tagactgtgc cctcgatccc ctgtccgaaa ctaaatgcac actcaagtcc
      901 ttcacagttg agaaaggcat ataccaaacc agtaacttcc gcgtccaacc caccgaatcc
      961 attgttcgat tccctaacat caccaatttg tgtcctttcg atgaagtatt taatgccact
     1021 cgatttgcat cagtttatgc ctggaatcgc aagcgaatat caaattgtgt tgcagattat
     1081 agtgtcctgt ataatttcgc accattcttc gcttttaagt gttatggggt cagccctacg
     1141 aaattgaatg acttgtgttt taccaacgtt tacgcagatt ctttcgtcat acgcggcaac
     1201 gaagtcagcc agattgcacc ggggcaaacc ggaaatatag cagactataa ttataagttg
     1261 ccggatgact tcaccggctg cgttatagca tggaactcca ataagctcga ttcaaaagtc
     1321 ggcggcaact ataattatcg ttaccgcctg tttcgcaagt caaatctgaa gccctttgaa
     1381 cgtgacatct ccaccgaaat ttatcaagct ggcaacaaac catgtaatgg agtggcaggg
     1441 gttaactgtt attttcccct tcaatcatac ggatttcgac caacatatgg ggtgggccat
     1501 caaccatatc gggtggtagt cttgagcttt gagctgctcc acgcaccggc tacagtttgc
     1561 ggaccgaaga aaagcactaa cctggtgaag aataaatgtg tgaacttcaa ctttaatggg
     1621 ctcaccggaa caggggtgct gacagaatca aacaagaaat ttctcccttt tcaacaattt
     1681 ggtagagaca ttgccgatac cacagatgcc gtccgcgatc cgcaaacact ggaaatcctc
     1741 gatataacac cctgtagctt cggcggggtc agcgtgataa ctcccggaac gaacacatcc
     1801 aaccaagtgg ctgtcctgta tcaaggtgtc aactgtaccg aagtccctgt ggcgatacac
     1861 gccgaccaac tgacacccac ctggagagtc tactcaacag gttccaatgt ctttcaaact
     1921 agagcgggtt gtctcattgg cgctgagtat gtcaatagtt catacgaatg tgatattcct
     1981 attggggcag gaatctgtgc gtcttatcaa actcaaacga aatcacaccg gcgcgctcgc
     2041 tcagtagcca gccagtccat aatcgcctat accatgagtc tcggggctga gaactccgtg
     2101 gcttattcaa acaattccat cgcaatccca actaacttta ccattagcgt taccacggaa
     2161 atcttgcccg tcagcatgac taagacgagc gtagactgta cgatgtatat ctgtggggat
     2221 tccaccgagt gtagcaacct cttgcttcaa tacgggtctt tctgtactca actcaaacga
     2281 gcattgaccg gaattgcggt cgaacaagac aagaataccc aagaggtgtt cgcacaagta
     2341 aagcaaatat acaaaacgcc gccaatcaaa tattttggcg ggtttaattt tagccaaata
     2401 ctgccagacc catctaagcc ttcaaaacgc agctttatcg aggatctgct gttcaacaaa
     2461 gttacgttgg ctgacgcggg tttcattaag cagtatgggg attgtttggg agacattgcg
     2521 gcccgcgacc ttatctgtgc tcaaaaattc aatgggttga cagttctgcc ccctctgctc
     2581 actgatgaga tgattgcaca gtatacatca gcattgttgg caggaaccat tacatccggc
     2641 tggacttttg gcgcgggtgc ggcccttcaa atacctttcg ctatgcaaat ggcttacaga
     2701 ttcaatggga ttggtgtcac gcaaaatgta ttgtatgaaa atcagaagct gatcgctaat
     2761 caatttaaca gcgcgattgg caagatccaa gattcactgt caagcacagc gtccgccctc
     2821 ggcaaactcc aagatgttgt gaatcataac gcccaggccc tgaataccct cgtgaagcaa
     2881 ctgagttcta aattcggcgc aatctccagc gtcctcaacg acatattgtc cagacttgat
     2941 aaggtggaag ccgaggtgca aattgatcgg ctcatcacag gcaggctcca gtcactccaa
     3001 acttatgtga cacaacagtt gatccgggct gctgagatac gagcaagcgc caacctggcc
     3061 gccactaaaa tgtcagagtg cgtgctcggg cagtccaaac gggttgactt ttgtggcaag
     3121 ggctatcact tgatgtcctt cccccaatcc gccccacatg gagtggtctt tctccacgtc
     3181 acttatgtcc ctgctcaaga gaagaacttt acgacagctc cagcaatctg tcacgatggc
     3241 aaagcgcact ttcccaggga aggcgttttc gtcagtaatg gcacgcattg gtttgtgacc
     3301 caaaggaatt tctatgaacc ccaaatcata accactgaca atacatttgt ctcaggcaac
     3361 tgtgatgtgg tcattgggat cgtgaacaac acggtatatg atcccctgca acccgagttg
     3421 gatagcttca aagaggaatt ggataaatac tttaagaatc atacttcacc cgatgttgat
     3481 ctcggggaca tatcaggaat aaatgcaagt gtcgtcaaca ttcagaaaga gattgatcgc
     3541 cttaatgagg tcgccaagaa cttgaatgaa tctttgatcg acttgcaaga actgggcaaa
     3601 tacgaacaat atattaaatg gccctggtac atttggttgg gctttatcgc cggacttata
     3661 gctatcgtta tggtcacaat tatgttgtgc tgtatgacta gctgttgttc ctgcttgaaa
     3721 ggatgttgca gttgcggatc ttgttgtaaa taaaggatcc tctagaagtt gtctcctcct
     3781 gcactgactg actgatacaa tcgatttctg gatccgcagg cctaatcaac ctctggatta
     3841 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3901 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3961 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4021 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4081 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4141 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4201 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4261 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4321 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4381 gagtcggatc tccctttggg ccgcctcccc gcctgtctag cttgactgac tgagatacag
     4441 cgtaccttca gctcacagac atgataagat acattgatga gtttggacaa accacaacta
     4501 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4561 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4621 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg
     4681 gcccatctct atcggtatcg tagcataacc ccttggggcc tctaaacggg tcttgagggg
     4741 ttttttgtgc ccctcgggcc ggattgctat ctaccggcat tggcgcagaa aaaaatgcct
     4801 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt
     4861 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taaggtcgtc
     4921 agctatcctg caggcgatct ctcgatttcg atcaagacat tcctttaatg gtcttttctg
     4981 gacaccacta ggggtcagaa gtagttcatc aaactttctt ccctccctaa tctcattggt
     5041 taccttgggc tatcgaaact taattaagcg atctgcatct caattagtca gcaaccatag
     5101 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5161 cccatcgctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5221 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ggaggtagcc
     5281 aacatgattg aacaagatgg attgcacgca ggttctcccg ccgcttgggt ggagaggcta
     5341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5461 ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc
     5821 gacggcgagg atctcgtcgt gacccacggc gatgcctgct tgccgaatat catggtggaa
     5881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6061 cttgacgagt tcttctagta tgtaagccct gtgccttcta gttgccagcc atctgttgtt
     6121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6301 gtgggctcta tggttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg
     6361 gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc
     6421 gttctccgat tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga
     6481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7321 catccatagt tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa
     7381 cctttcgtcc gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc
     7441 cggccttgcg ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg
     7501 aatatccgag atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat
     7561 ccgagatccg aggaatatcg aaatcggggc gcgcctggtg taccgagaac gatcctctca
     7621 gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa tccagcttgg
     7681 gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca gcgtaccgat
     7741 ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt
     7801 gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat tcaacatttc
     7861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg ttacatcgaa
     7981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ctttccaatg
     8041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ttcaccagtc
     8161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8221 atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc gaaggagcta
     8281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8401 accttgcgta aactattaac tggcgaacta cttactctag cttcccggca acagttgata
     8461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8701 taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta
     8761 tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat
     8821 acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa ctcgactctg
     8881 gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct catttgctcg
     8941 tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga tcgcggctcc
     9001 cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc ataacagcag
     9061 cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
     9121 atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc ttcgatggat
     9181 agcgatttta ttatctaact gctgatcgag tgtagccaga tctagtaatc aattacgggg
     9241 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9421 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     9541 cagtacatct acgtattagt catcgctatt accatgctga tgcggttttg gcagtacatc
     9601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     9661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     9721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     9781 ggtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     9841 agacaccggg accgatccag cctcccctcg aagcttacat gtggtaccga gctcggatcc
     9901 tgagaacttc agggtgagtc tatgggaccc ttgatgtttt ctttcccctt cttttctatg
     9961 gttaagttca tgtcatagga aggggagaag taacagggta cacatattga ccaaatcagg
    10021 gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact tttttgttta
    10081 tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat acaatgtatc
    10141 atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa ggcaatagca
    10201 atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga ggtttcatat
    10261 tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt gggataaggc
    10321 tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc tcttatcttc
    10381 ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt tggcaaaggc
    10441 ggccgcc
//
