LOCUS       sequence-432852-        9897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV
            or T7 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-PCV1...
FEATURES             Location/Qualifiers
     source          1..9897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2474..4987,4988..4990,4991..6574)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2474..6574
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6632..6652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8537..8557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8566..8583
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8606..8623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8612..8836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8907..8923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8907..8923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8920..8942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8931..8947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8955..8961,8962..8979,8980..8985))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9000..9021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9138..9155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9309..9897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9389..9408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gcgaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgcctag caagaagtcc ggcccccagc cacataagcg
     2101 gtgggtgttc accctgaaca acccctctga ggaagaaaag aacaagatca gagagctgcc
     2161 tatcagcctg ttcgactact ttgtgtgtgg agaagagggc ctggaagagg gaagaacccc
     2221 tcacctgcaa ggctttgcca atttcgccaa gaaacagacc ttcaacaaag tgaagtggta
     2281 cttcggcgct agatgccaca tcgagaaggc caagggcaca gatcagcaga ataaggaata
     2341 ctgcagcaaa gagggccaca tcctgatcga gtgcggcgcc cctagaaacc agggcaagcg
     2401 gagcggaggc tctagcggct ccgagacacc cggcacatct gagtccgcca cccctgagag
     2461 cagcggcggc agcgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
     2521 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     2581 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     2641 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     2701 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     2761 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     2821 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     2881 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     2941 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     3001 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     3061 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     3121 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     3181 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     3241 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     3301 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     3361 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     3421 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     3481 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     3541 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     3601 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     3661 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     3721 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     3781 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     3841 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     3901 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     3961 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     4021 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     4081 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     4141 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     4201 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     4261 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     4321 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     4381 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     4441 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     4501 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     4561 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     4621 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     4681 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     4741 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     4801 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     4861 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     4921 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     4981 tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     5041 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     5101 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     5161 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     5221 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     5281 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     5341 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     5401 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     5461 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     5521 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     5581 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     5641 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     5701 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     5761 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     5821 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     5881 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     5941 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     6001 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     6061 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     6121 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     6181 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     6241 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     6301 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     6361 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     6421 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     6481 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     6541 gacacggatc gacctgtctc agctgggagg tgactccggc ggaagctctg gtggcagcaa
     6601 gcggaccgcc gacggctctg aattcgagag ccctaagaag aaaagaaagg tgagcggagg
     6661 ctctagcggc ggaagcgtta tctcctacga caactacgtg accatcctgg acgaagagac
     6721 actgaaggct tggatcgcta aactggaaaa agcccctgtg ttcgccttcg ccaccgccac
     6781 cgactcctta gataacatca gcgccaacct ggtgggcctg tctttcgcca tcgagcctgg
     6841 cgtggccgcc tacatccccg tggcccacga ttacctggat gccccagatc aaatcagcag
     6901 agagagagca ctggagctcc tgaagcccct gctggaggac gagaaggccc tgaaggtggg
     6961 acagaacctg aaatacgata ggggaatcct ggctaattac ggcatcgagc tgagaggcat
     7021 cgccttcgat accatgctgg aatcttacat cctgaacagc gtggccggcc gccacgacat
     7081 ggacagcctg gctgaacggt ggctgaagca caaaaccatc accttcgaag agatcgccgg
     7141 aaagggcaag aaccagctga catttaatca gatcgcactg gaagaagccg gcagatacgc
     7201 cgctgaggac gccgacgtga cccttcagct gcacctgaag atgtggcccg atctgcagaa
     7261 gcacaagggc ccactaaacg tgttcgagaa catcgagatg cctctggtgc ccgtcctgag
     7321 cagaatcgag aggaatggcg tgaagatcga ccccaaggtg ctgcacaacc acagcgagga
     7381 actgacactg cggctggccg agctggaaaa gaaggcccac gaaatcgccg gcgaggagtt
     7441 caacctgtct agcaccaagc agctacaaac aatcctgttc gagaagcagg gcattaagcc
     7501 tctgaaaaag acacctggcg gagcccccag caccagcgaa gaagtgctgg aggaactggc
     7561 gctggattac cctctgccca aagtgatcct ggaatataga ggactggcca agctgaagtc
     7621 tacatacacc gacaagctgc ctctgatgat caaccccaag accggccggg tgcatacatc
     7681 ttatcaccag gccgtgacag ccaccggccg gctgagcagc acagatccta acctgcaaaa
     7741 catcccagtg cggaacgaag agggcagaag aatcagacag gctttcattg cccctgagga
     7801 ctacgtcatc gtgtctgctg actacagcca gatagaactg agaatcatgg cccatctgtc
     7861 tcgggacaag ggcctgctga ccgcttttgc agaaggcaag gacatccaca gagccaccgc
     7921 tgccgaagtg ttcggcctgc ctctggaaac agtgaccagc gagcaaagaa ggtccgccaa
     7981 ggccattaac ttcggcctga tctacggcat gagcgccttt ggcctggcca gacagctcaa
     8041 catccctaga aaggaggccc agaaatacat ggacctgtac ttcgagcggt accccggcgt
     8101 gctggagtac atggaacgga ccagagccca ggctaaagag cagggatacg tggaaaccct
     8161 ggatggccgg agactgtatc tgcctgacat caagagcagc aacggcgcca gaagagccgc
     8221 cgctgagaga gctgctatca acgcccctat gcagggcacc gccgccgaca tcatcaagcg
     8281 ggccatgatc gccgtggacg cctggctgca ggccgagcag cctagagtgc ggatgatcat
     8341 gcaggtgcac gacgagctgg tgtttgaggt gcacaaggac gacgtggatg ctgtggccaa
     8401 gcagattcac cagctgatgg aaaattgcac cagactggac gtgccactgc tggtcgaggt
     8461 tggaagcgga gagaattggg accaggccca ctctggcggc tcaaaaagaa ccgccgacgg
     8521 cagcgaattc gagtctccca agaagaagag gaaagtctga ccggtcatca tcaccatcac
     8581 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8641 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8701 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8761 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc
     8881 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8941 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa
     9001 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9061 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9721 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaa
//
