LOCUS       sequence-432853-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Variant CE1 construct with StCV HUH endonuclease, expressed from CMV
            or T7 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-StCV...
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(296..315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        487..866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        867..1070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1021..1041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1112..1131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1112..1130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1138..1147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1537..4050,4051..4053,4054..5637)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1537..5637
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5695..5715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7600..7620
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7629..7646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7669..7686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7675..7899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7970..7986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7970..7986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7983..8005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7994..8010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8018..8024,8025..8042,8043..8048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8063..8084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8201..8218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8372..8960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8452..8471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9131..9879,1..43,44..112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9754..9773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa
      301 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      361 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      421 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      481 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      541 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      601 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      661 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      721 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      781 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      841 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      901 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      961 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     1021 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     1081 accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc
     1141 accatggccg tgcggggctc cgccgctaaa agatggtgct tcaccctgaa caaccccacc
     1201 gaggaagaaa tcgccgccgt caaggcctgg cagcacagcg agtaccacta cgccatcgtg
     1261 ggcaaggaaa agggcgagca gggaacacct catctgcagg gcttcatcca cctgaaaaag
     1321 aaggtgcggc tgaccagcct gaagaaagtg ctgcaaagag cccactggga gaaggctaga
     1381 ggctctgatg aggacaacga gaagtactgt agcaaggaag gcgacgtgat cctgacaatc
     1441 ggcattcctg tgaagggaaa tagaagcgga ggctctagcg gctccgagac acccggcaca
     1501 tctgagtccg ccacccctga gagcagcggc ggcagcgaca agaagtacag catcggcctg
     1561 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     1621 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     1681 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     1741 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     1801 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     1861 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     1921 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     1981 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     2041 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     2101 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     2161 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     2221 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     2281 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     2341 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     2401 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     2461 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     2521 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     2581 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     2641 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     2701 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     2761 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     2821 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     2881 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     2941 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     3001 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     3061 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     3121 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     3181 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     3241 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     3301 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     3361 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     3421 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     3481 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     3541 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     3601 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     3661 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     3721 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     3781 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     3841 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     3901 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     3961 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     4021 atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt tctgaaggac
     4081 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     4141 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     4201 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     4261 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     4321 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     4381 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     4441 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     4501 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     4561 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     4621 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     4681 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     4741 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     4801 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     4861 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     4921 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     4981 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     5041 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     5101 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     5161 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     5221 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     5281 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     5341 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     5401 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     5461 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     5521 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     5581 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactcc
     5641 ggcggaagct ctggtggcag caagcggacc gccgacggct ctgaattcga gagccctaag
     5701 aagaaaagaa aggtgagcgg aggctctagc ggcggaagcg ttatctccta cgacaactac
     5761 gtgaccatcc tggacgaaga gacactgaag gcttggatcg ctaaactgga aaaagcccct
     5821 gtgttcgcct tcgccaccgc caccgactcc ttagataaca tcagcgccaa cctggtgggc
     5881 ctgtctttcg ccatcgagcc tggcgtggcc gcctacatcc ccgtggccca cgattacctg
     5941 gatgccccag atcaaatcag cagagagaga gcactggagc tcctgaagcc cctgctggag
     6001 gacgagaagg ccctgaaggt gggacagaac ctgaaatacg ataggggaat cctggctaat
     6061 tacggcatcg agctgagagg catcgccttc gataccatgc tggaatctta catcctgaac
     6121 agcgtggccg gccgccacga catggacagc ctggctgaac ggtggctgaa gcacaaaacc
     6181 atcaccttcg aagagatcgc cggaaagggc aagaaccagc tgacatttaa tcagatcgca
     6241 ctggaagaag ccggcagata cgccgctgag gacgccgacg tgacccttca gctgcacctg
     6301 aagatgtggc ccgatctgca gaagcacaag ggcccactaa acgtgttcga gaacatcgag
     6361 atgcctctgg tgcccgtcct gagcagaatc gagaggaatg gcgtgaagat cgaccccaag
     6421 gtgctgcaca accacagcga ggaactgaca ctgcggctgg ccgagctgga aaagaaggcc
     6481 cacgaaatcg ccggcgagga gttcaacctg tctagcacca agcagctaca aacaatcctg
     6541 ttcgagaagc agggcattaa gcctctgaaa aagacacctg gcggagcccc cagcaccagc
     6601 gaagaagtgc tggaggaact ggcgctggat taccctctgc ccaaagtgat cctggaatat
     6661 agaggactgg ccaagctgaa gtctacatac accgacaagc tgcctctgat gatcaacccc
     6721 aagaccggcc gggtgcatac atcttatcac caggccgtga cagccaccgg ccggctgagc
     6781 agcacagatc ctaacctgca aaacatccca gtgcggaacg aagagggcag aagaatcaga
     6841 caggctttca ttgcccctga ggactacgtc atcgtgtctg ctgactacag ccagatagaa
     6901 ctgagaatca tggcccatct gtctcgggac aagggcctgc tgaccgcttt tgcagaaggc
     6961 aaggacatcc acagagccac cgctgccgaa gtgttcggcc tgcctctgga aacagtgacc
     7021 agcgagcaaa gaaggtccgc caaggccatt aacttcggcc tgatctacgg catgagcgcc
     7081 tttggcctgg ccagacagct caacatccct agaaaggagg cccagaaata catggacctg
     7141 tacttcgagc ggtaccccgg cgtgctggag tacatggaac ggaccagagc ccaggctaaa
     7201 gagcagggat acgtggaaac cctggatggc cggagactgt atctgcctga catcaagagc
     7261 agcaacggcg ccagaagagc cgccgctgag agagctgcta tcaacgcccc tatgcagggc
     7321 accgccgccg acatcatcaa gcgggccatg atcgccgtgg acgcctggct gcaggccgag
     7381 cagcctagag tgcggatgat catgcaggtg cacgacgagc tggtgtttga ggtgcacaag
     7441 gacgacgtgg atgctgtggc caagcagatt caccagctga tggaaaattg caccagactg
     7501 gacgtgccac tgctggtcga ggttggaagc ggagagaatt gggaccaggc ccactctggc
     7561 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagtctc ccaagaagaa gaggaaagtc
     7621 tgaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     7681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7861 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7921 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8041 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9241 catctggccc cagtgctgca atgataccgc gcgaaccacg ctcaccggct ccagatttat
     9301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9841 tgttgagatc cagttcgatg taacccactc gtgcaccca
//
