LOCUS       sequence-432864-       10155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Variant CE1 construct with Cdc13(Y556A) telomere binding protein for
            template recruitment, expressed from CMV or T7 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: Click editor utilizing Cdc13(Y556A) for templa...
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2732..5245,5246..5248,5249..6832)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2732..6832
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6890..6910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8795..8815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8824..8841
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8864..8881)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8870..9094
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9165..9181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9165..9181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9178..9200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9189..9205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9213..9219,9220..9237,9238..9243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9258..9279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9396..9413)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9567..10155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9647..9666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gcgaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgagaat gagcaaaatg gccagaaagg accctaccat
     2101 cgagttctgt cagctgggcc tcgacacatt cgagacaaag tacatcacca tgttcggcat
     2161 gctggtcagc tgcagcttcg acaagcctgc cttcatcagc tttgtgttca gcgacttcac
     2221 caagaacgac atcgtgcaga acgccctgta cgacagatac ctgatcgact acgagaacaa
     2281 gctggaactg aacgagggct tcaaggccat catgtataag aaccagttcg aaacctttga
     2341 tagcaagctg agaaagatct tcaacaacgg actgagggat ctgcagaatg gaagagatga
     2401 gaatctgagc caatacggca tcgtgtgcaa gatgaacatc aaggtgaaga tgtacaacgg
     2461 caaactgaat gccattgtgc gggaatgtga acccgtgccc cattctcaga tctctagcat
     2521 cgcctctcct agccagtgcg agcacctgcg gctgttctac cagcgcgcct ttaaaagaat
     2581 cggcgagagc gctatcagcc ggtactttga ggaataccgg agattcttcc ccatccacag
     2641 aaacggctcc cacctggcca gcggaggctc tagcggctcc gagacacccg gcacatctga
     2701 gtccgccacc cctgagagca gcggcggcag cgacaagaag tacagcatcg gcctggacat
     2761 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     2821 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     2881 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     2941 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     3001 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     3061 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     3121 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     3181 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     3241 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     3301 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     3361 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     3421 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     3481 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     3541 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     3601 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     3661 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     3721 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     3781 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     3841 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     3901 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     3961 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     4021 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     4081 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     4141 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     4201 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     4261 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     4321 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     4381 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     4441 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     4501 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     4561 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     4621 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     4681 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     4741 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     4801 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     4861 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     4921 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     4981 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     5041 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     5101 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     5161 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     5221 ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga aggacgactc
     5281 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     5341 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     5401 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     5461 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     5521 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     5581 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     5641 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     5701 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     5761 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     5821 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     5881 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     5941 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     6001 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     6061 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     6121 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     6181 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     6241 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     6301 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     6361 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     6421 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     6481 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     6541 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     6601 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     6661 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     6721 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     6781 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg actccggcgg
     6841 aagctctggt ggcagcaagc ggaccgccga cggctctgaa ttcgagagcc ctaagaagaa
     6901 aagaaaggtg agcggaggct ctagcggcgg aagcgttatc tcctacgaca actacgtgac
     6961 catcctggac gaagagacac tgaaggcttg gatcgctaaa ctggaaaaag cccctgtgtt
     7021 cgccttcgcc accgccaccg actccttaga taacatcagc gccaacctgg tgggcctgtc
     7081 tttcgccatc gagcctggcg tggccgccta catccccgtg gcccacgatt acctggatgc
     7141 cccagatcaa atcagcagag agagagcact ggagctcctg aagcccctgc tggaggacga
     7201 gaaggccctg aaggtgggac agaacctgaa atacgatagg ggaatcctgg ctaattacgg
     7261 catcgagctg agaggcatcg ccttcgatac catgctggaa tcttacatcc tgaacagcgt
     7321 ggccggccgc cacgacatgg acagcctggc tgaacggtgg ctgaagcaca aaaccatcac
     7381 cttcgaagag atcgccggaa agggcaagaa ccagctgaca tttaatcaga tcgcactgga
     7441 agaagccggc agatacgccg ctgaggacgc cgacgtgacc cttcagctgc acctgaagat
     7501 gtggcccgat ctgcagaagc acaagggccc actaaacgtg ttcgagaaca tcgagatgcc
     7561 tctggtgccc gtcctgagca gaatcgagag gaatggcgtg aagatcgacc ccaaggtgct
     7621 gcacaaccac agcgaggaac tgacactgcg gctggccgag ctggaaaaga aggcccacga
     7681 aatcgccggc gaggagttca acctgtctag caccaagcag ctacaaacaa tcctgttcga
     7741 gaagcagggc attaagcctc tgaaaaagac acctggcgga gcccccagca ccagcgaaga
     7801 agtgctggag gaactggcgc tggattaccc tctgcccaaa gtgatcctgg aatatagagg
     7861 actggccaag ctgaagtcta catacaccga caagctgcct ctgatgatca accccaagac
     7921 cggccgggtg catacatctt atcaccaggc cgtgacagcc accggccggc tgagcagcac
     7981 agatcctaac ctgcaaaaca tcccagtgcg gaacgaagag ggcagaagaa tcagacaggc
     8041 tttcattgcc cctgaggact acgtcatcgt gtctgctgac tacagccaga tagaactgag
     8101 aatcatggcc catctgtctc gggacaaggg cctgctgacc gcttttgcag aaggcaagga
     8161 catccacaga gccaccgctg ccgaagtgtt cggcctgcct ctggaaacag tgaccagcga
     8221 gcaaagaagg tccgccaagg ccattaactt cggcctgatc tacggcatga gcgcctttgg
     8281 cctggccaga cagctcaaca tccctagaaa ggaggcccag aaatacatgg acctgtactt
     8341 cgagcggtac cccggcgtgc tggagtacat ggaacggacc agagcccagg ctaaagagca
     8401 gggatacgtg gaaaccctgg atggccggag actgtatctg cctgacatca agagcagcaa
     8461 cggcgccaga agagccgccg ctgagagagc tgctatcaac gcccctatgc agggcaccgc
     8521 cgccgacatc atcaagcggg ccatgatcgc cgtggacgcc tggctgcagg ccgagcagcc
     8581 tagagtgcgg atgatcatgc aggtgcacga cgagctggtg tttgaggtgc acaaggacga
     8641 cgtggatgct gtggccaagc agattcacca gctgatggaa aattgcacca gactggacgt
     8701 gccactgctg gtcgaggttg gaagcggaga gaattgggac caggcccact ctggcggctc
     8761 aaaaagaacc gccgacggca gcgaattcga gtctcccaag aagaagagga aagtctgacc
     8821 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     8881 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8941 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9001 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9061 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9121 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9241 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9361 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10141 aaaaaaggat ctcaa
//
