LOCUS       sequence-432942-        2782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive promoter for gene expression in S. pombe. Part type 2 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM020_P2_pTRS1
FEATURES             Location/Qualifiers
     source          1..2782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1650..1684)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1755..2414)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2329..2348
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2596..2782,1..402)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
       61 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      481 cctgattctg tggataaccg tagtcggtct caaacgcaaa gtctattaac ttcaactttc
      541 ttacctctga caagatgttt cttcgacagg tcagaaatcg atgcccaaaa tcaaattttg
      601 aaggtacttt gcttatggtt caaaattatc tagccaagta taaccatcat gattgcgaga
      661 gtcaaattca gtactgcgtt gaaaggaaac acagtacttc actatgaaat gtttaactat
      721 ataatgatgc attatgctgc ggtgagtcaa cttgtgactt agtacattag attatgcatt
      781 gctatagaaa gtttgcgtag agagattttg tattaaagat cagcgtaaca gacaattgag
      841 tttagatatt cttctttttt acctttcata tcataggtct acatctcttg gtgtatagct
      901 aaataatgtc ttttcaatgt cataattaac cagcatttta atttatattt gaaatataca
      961 atgaatagtg aaagttgcgc aatgtaagct acgcagtttg gtatctgatt taaggatacg
     1021 tagaactgcg gtgagttttc cttgtgatca attatattac aatacacagg ttgtataagt
     1081 agcaactgag tataggtatt gtattaactg ggttataatg ttacctatca ctaatatagc
     1141 tcataactga actgaggaac gaggttcagc agtagctcta tttatattat tcagataata
     1201 tagatcttaa tagattatca ttaagatcaa tctccatatc gtatcataca tggtacgata
     1261 tataattaga acatgtgaca tatagtgata cgcaacgtag tgtatcgtag cgtagtgtag
     1321 tattgctgac acgcaaaaat atatagtgct gcttggtgac gcttaataac gaatagcagt
     1381 gcggtactca acaccaaaac gcaatgtgac agcgaaattt tcgttgtaac tcgaaaagtg
     1441 tactttggaa tctgtcctaa caccatacca tcagtagtct aactgccaag ctgaaatgag
     1501 ttttagaggc ggtcgcggtg gtggatttag aggaggacga ggtggatctc gtccttttac
     1561 tccttcaggt ccacccgatc aagtcattgg taagaaaaaa acaagaggaa attttccagt
     1621 gcctaagaga tctatgtgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1681 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1741 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1801 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1861 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1921 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1981 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2041 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2101 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2161 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     2221 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2281 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2341 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2401 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     2461 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     2521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2761 gttaggccac cacttcaaga ac
//
