LOCUS       sequence-432944-        2774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive promoter for gene expression in S. pombe. Part type 2 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM024_P2_pMPA1
FEATURES             Location/Qualifiers
     source          1..2774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             609..1268
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(675..694)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1339..1373
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(join(2613..2774,1..427))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2693..2712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
       61 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      481 acgttaaggg attttggtca tgattgatcg ggcacgtaag aggttccaac tttcaccata
      541 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      601 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      661 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      721 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      781 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      841 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      901 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      961 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1021 ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt
     1081 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1141 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1201 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1261 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     1321 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg
     1381 gtctcacata gatctgagca aattcagcag cttcctcacg gttcttctca gcttgtctgc
     1441 gcttgagggc aaagcggtga cgcttgtgtt gaagggttct gggagtgaca agacgttgaa
     1501 tcttgggagc cttggtatag ggcttcttgc cctccttttt gggaacgacc tcacgacgaa
     1561 taacgaattg gcgaacatcg tcttccttgg acaagttgaa gaaacgacga atcttagaag
     1621 cacgcttggg gccaagacgc ttgggaacag taacatcggt caaaccaggg atgtcttgct
     1681 caccttgctt aacaatggca agggcgagga cagccaaatc ttgaccaacg atgcaaccgc
     1741 ggacagactt acgcttgcgc tcaccatcac gacgaggacg gtagcaaggg tgaccggcac
     1801 ggagtaaaag acggacacgg tgaggaagca aaacaccctg gaacattggg aaaccttgct
     1861 tatcgttacc accggtaatc ttgaagacat aaccagcaaa ctcaggtcct acagagtcac
     1921 ctgggacctc ctgtcccatt ctcttctcca taaacacgcg aagacgacgg tcatcgtcaa
     1981 tttcgatcag cttttgagta ccattagccg ggtaggaaat gttcagcttc atcgtggcgg
     2041 ttgggaaatt cctattcttg tcacattctt gatccttcaa cgcacttttt taaacagtca
     2101 caatgttcga ttaaaaaccc taccctgttt acttgatcgt cgccctttat atcttaacaa
     2161 ttcgttattt aagctaaacg tatgattacg tactgcaata tccgcgatta cgataaatcc
     2221 cacatcagtt agctatatct gtaaatcaga ttaccactcc cttacagata tgttgtaagt
     2281 aataacactg cggtaggatt ctcgttacgg tggtaaataa ctacttcgag attggctaga
     2341 gctagataaa actttggtct tctataaatt atcttttatt ttattttttt tatttggaaa
     2401 acgtttatgt aatggtcaac aaacactcgt attttagtat gtttttgagc aatcatggaa
     2461 tttttatgca atatttgtaa atcgctaaca gaaacccccg tttgagaccg actacggtta
     2521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2761 ggatacctgt ccgc
//
