LOCUS       sequence-432959-        3862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' homology arm for S. pombe genomic integration. Part type 8b 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM040_P8b_5'Ura4
FEATURES             Location/Qualifiers
     source          1..3862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     260..279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1439..2233
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     terminator      complement(2687..2721)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2792..3451)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3366..3385
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(3633..3862,1..359)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
       61 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      121 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      181 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      241 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      301 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      361 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      421 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcac aatatgctta
      481 cataatgagt gagtgtcatt attgaggaat gccatgtcag atttgacaca actcagaact
      541 tattaacttt ttatgattgg aacttttgtt ttgcccaagg aatttttaat ggaagaagtt
      601 gttttataaa tgatattgaa tttatatcca ctctttttag cgcatttgca tctctacttt
      661 aggttcttct ttgactagat tctaggatgc gtattggata tactggtctt tgaatcattg
      721 tcgaaaaaaa aattcatggt tgtacccatg agcaaactgc ttttgtggtt atccatgtgc
      781 ttcttttgtt cagcatctat cttcttaatc gcatggaaga tttgatttaa accaaaagaa
      841 atattgttaa actttgacag aagctttaaa ttttgattcc atacattaaa tggataaaac
      901 tcctgaagct tagctacaaa tcccactggc tatatgtatg catttgtgtt aaaaaagttt
      961 gtatagatta tttaatctac tcagcattct ttctctaaat aggaatttgt tacttaatgg
     1021 agaaaaaaat gtttcgattt acctagtgta tttgtttgta tactcacgtt taatttcaaa
     1081 catccattct atcttgtgta atttttggca tggtgaaaaa gataatcagc cttataatct
     1141 ttacaaaagt aagaaattct gtaaataagc cttaatgccc ttgctttaaa ttaaaatggt
     1201 tctttttcat gataatgttt gcactttgtg aatatatttt agatagttct gtgaggtata
     1261 attaagatgt tttagagact tatacaattt tgtctttata aattcttaat tgattttacc
     1321 atcccagttt aactatgctt cgtcggcatc tctgcacatg tcgtgttttc ttaccgtatt
     1381 gtcctaccaa gaacctcttt tttgcttgga tcgaaattaa aggtttaaaa gcaaagttat
     1441 ggatgctaga gtatttcaaa gctattcagc tagagctgag gggatgaaaa atcccattgc
     1501 caaggaattg ttggctttga tggaagaaaa gcaaagcaac ttgtcagtcg cggtcgattt
     1561 gacgaagaaa tccgaaatct tagaattggt agataaaatt ggaccctatg tctgtgttat
     1621 caagacacat attgacgttg tcgaggattt cgaccaggat atggtagaaa aactggtggc
     1681 cttaggtaaa aagcatcgtt ttcttatctt tgaggatcgc aaattcgcag acattggaaa
     1741 taccgtcaag ctacaatatg catctggtgt gtacaaaatt gcttcttggg ctcatatcac
     1801 aaattgccat acagtgccag gcgagggtat tatacaaggc ctcaaagaag ttggtttacc
     1861 tttgggacgt ggactcttgc ttttggctga aatgtcttcc aaaggctctt tggctactgg
     1921 ttcctacaca gagaaaacct tagaatggtt tgagaagcat accgattttt gctttggctt
     1981 tatagctggt cgtcgatttc ctaaccttca aagcgactac ataactatgt cccctggtat
     2041 cggcttggat gttaaaggtg acgggctggg acagcaatat cgtactcctg aagaagtgat
     2101 tgtaaactgc ggtagcgata tcatcattgt tggtcgtgga gtctatggag ctggtcgtaa
     2161 tcctgttgtc gaagccaaga gatatagaga agctggttgg aaggcatatc agcaaagact
     2221 ttctcagcat taaaaaaaga ctaatgtaaa atttttttgg ttggttattg aaaaagtcga
     2281 tgccttgttt gcgtttgttt tcctaggcgt tttatgtcag aaggcattta gaattagtat
     2341 acaagtactc tttggtaaaa ttttatgtag cgactaaaat attaactatt atagataaac
     2401 accttgggaa taaaaagtaa tttgctatag taatttatta aacatgctcc tacaacatta
     2461 ccacaatctt ttctcttgga ttgacattga ataagaaaag agtgaatttt tttagacttg
     2521 taatgataac tatgtacaaa gccaatgaaa gatgtatgta gatgaatgta aaataccatg
     2581 tagacaaaca agataaaact tggttataaa cattggtgtt ggaacagaat aaattagatg
     2641 tcaaaaagtt tcgtcaatat cacaagcttc ccttgagacc agaccaataa aaaacgcccg
     2701 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     2761 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     2821 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     2881 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     2941 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     3001 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     3061 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     3121 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     3181 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     3241 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     3301 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     3361 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     3421 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     3481 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     3541 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt ac
//
