LOCUS       sequence-432969-        2152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' homology arm for S. pombe genomic integration. Part type 7 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM036_P7_3""Ura4
FEATURES             Location/Qualifiers
     source          1..2152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(841..875)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(946..1605)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1520..1539
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1787..2152,1..223)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
       61 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      241 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      301 ccctgattct gtggataacc gtagtcggtc tcagagttta ttattcaaaa ctagtatact
      361 ttttctcgga gtataataca caatatcggt gcaaataggt tttaaaattg ctccaatcac
      421 atgttcttaa gaaaaaaacg tcaaaagaaa tctaagtatt cccaaaataa ttcctcctga
      481 catgagaaga ataaaaaaca ctaagatgca ttgtcagtat gaaacacaag ccaaagttgc
      541 taacaatagg atcaattctt acttactggc aatgttacga ggaatcttgg taggaggatg
      601 tttggcaaca taacgttctt ttgaatttgt ttttacacga cgagtaatgt tcttctgaaa
      661 ctgtgtgttt gcacgacgct gagagggagt ttgaaccgtc taaaagacat agttaggatc
      721 catgatgcac agatttctaa agataaaact acttacaggc atgttggttt attaaaaaag
      781 aaaaacagga atgataagag ggaaagaata cgtgtacaga gcaccgatga gaccagacca
      841 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      901 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      961 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1021 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1081 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1141 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     1201 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1261 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1321 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1381 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1441 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1501 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1561 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1621 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1681 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     1741 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1801 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1861 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1921 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1981 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2041 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2101 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac ac
//
