LOCUS       sequence-433012-        9441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golgiphagy flux reporter keima-YIPF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH116_keima-YIPF3
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    124..357
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    854..979
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1026..1659
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1851..1870)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1920..1936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1920..1936)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1933..1955)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1944..1960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1968..1974,1975..1992,1993..1998))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2274..2291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2445..3033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2525..2544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3204..3995,3996..4064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3827..3846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4065..4169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4237..4255
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4374..4607
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4682..5270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4788..4799
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5197..5217
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5287..5886)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5370..5390)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5867..5886
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    5898..6450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5900..6467
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6060..6079)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6289..6306
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    6480..6504
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7555..7579)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(7595..8254,8255..8257,8258..8260))
                     /codon_start=1
                     /product="humanized monomeric Keima-Red fluorescent 
                     protein"
                     /label=hmKeima-Red
                     /note="mammalian codon-optimized"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     regulatory      8257..8266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8342..8366)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8363..8566)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8392..8412)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8567..8870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8922..9039
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9336..9380)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
       61 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
      121 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
      181 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
      241 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
      301 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
      361 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
      421 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
      481 tctcctcctc caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt
      541 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
      601 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
      661 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
      721 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
      781 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
      841 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
      901 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
      961 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     1021 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     1081 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     1141 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     1201 agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc
     1261 cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag
     1321 ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg
     1381 ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca
     1441 ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg
     1501 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc
     1561 ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
     1621 ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat
     1681 atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt
     1741 aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga
     1801 ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg
     1861 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt cgtaatcatg
     1921 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1981 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2041 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccacgggga gctttttgca
     2101 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     2161 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tgagcttgct gcattaatga
     2221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2881 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     3841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4261 cgtcttcaag aatgatctag ccctttcctt aattaacccg ggcctctcac tctctgatat
     4321 tcatttcttt gcaagttata aatactgaat aataagatga catgaactac tactgctaga
     4381 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4441 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4501 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4561 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     4621 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aaggatctca
     4681 ggcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
     4741 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
     4801 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
     4861 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
     4921 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
     4981 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
     5041 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
     5101 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
     5161 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
     5221 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt aggatccatc
     5281 gatgcatcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
     5341 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
     5401 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
     5461 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
     5521 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
     5581 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
     5641 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
     5701 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
     5761 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
     5821 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
     5881 ggtcatggtt gtggccatat tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt
     5941 tcggggggcc tagacgtttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag
     6001 gccccagatc agatcccata caatggggta ccttctgggc atccttcagc cccttgttga
     6061 atacgcttga ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact
     6121 ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc
     6181 tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc
     6241 aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg
     6301 cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc
     6361 tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc
     6421 cttattccaa gcggcttcgg ccagtaacgt tagggggggg gggcggaatt cgttatcgaa
     6481 ccactttgta caagaaagtt gggcagtgtg actgcagggt caccgcaaca gctttggctg
     6541 tggcgttgag gacggtggtg ggaagccgag cagcagggag catggcaggg atgtctctgg
     6601 ggaccctctg gatgggcggg atgttggggc cctccagtgt gtccaggatc ccctctacca
     6661 ctttgtggta ggcaaaatgc agatagagca ggaagagcat gtgtagggca gccagggtgc
     6721 cacagaggag cagccgctgt gtggggccca cggtccgaga caccaacact gctaccatgc
     6781 gcagtgtgga cagtccaccc accaacagcc agaagaggta gaagagggcg tggaggtgga
     6841 tattataggt gatgaacagg acaatgcaat gcccaaagag gccatagccc agcagtgcca
     6901 acatctgcag catggtgatc tgggcgttgc acaggtaggc aaggaagtaa atgaaggatg
     6961 agactcccag ccagtagccg aagcaggtgc caatggctgt gcccatcagg gtgccctccc
     7021 ggataatagt gtcagacgtc ttcatcccat ggagtaggat agcaaccaga gtgaagacca
     7081 gcatgagagg tccatagagt tcacctgcaa ttttctgggg gaagttgacc atcttgatag
     7141 ggatcatgga ctccaggagc ctgcttcgca cctgagcagg ctccacatca aagtagggtc
     7201 tgaggatgtc gatgttggcg tacaagctga aggccctgga ggcttgtctt ttcccagcct
     7261 gccacatctg atctgccacc tgccggctca gctgtccctt aaagcccttc atgcccagga
     7321 actctccatc ctcctcttca gcagcagctg catcagctgc atcagcgtct acttcttcct
     7381 cgcgcaggcg ctgatgcagc tcacccatat cctcgaagct agagcctgag gtatcatcca
     7441 tgttctccat gtcaatcaca gctgagcctc cgccctggat gttttcttcg aaccctcccc
     7501 attccgggcc agctccattt cgggcgccgc ccgccggcgc agctgtagtt gccatgccaa
     7561 cttttttgta caaacttgtt tgataacctc gagcgcccag cagggagtgg cgggcgatgg
     7621 cgatctcgca ctgctccacg ctggtgtagt cgcggttgtg gctggtcacg tccagcttgc
     7681 ggtcgatctc gtggcggccg ggcatcctca cgggcttctt ggccttgtag gtgctcttga
     7741 actcgcacag gtagtggccg ccgccctcca gcttcagggc catgtagtcg ttgccgatca
     7801 gcattccgtc gcgggcgaac aggcgctcgg tgctgggctc ccagccctgg gtcttcttct
     7861 gcatcacggg gccgttgggg gggaagttct cgccgctgat cttcacgttg tagatgaagc
     7921 agttgccctg gatgctgctg tcgttgctca cggtgcacac ggcgccgtcc tcgaagttca
     7981 tgctgcgctc ccaggtgtag ccctcgggga agctctgctt gaagtagtcg gggatgtcct
     8041 cggggtactt ggtgaagggg atgctgccgt actggagctg ggggctcagg atgtcccagg
     8101 cgaagggcag ggggccaccc ttggtcacgg tcagcttcac ggtctgctcg ccctcgtagg
     8161 gcttgccctt gccgtcgccc tccacctcga agtagtggcc gttcacggtg ccgctcatgt
     8221 acaccttgta ggtcatctgc ttggcgatca cgctcaccat ggtggcagat caacctcgag
     8281 agatctgcgg ccgccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
     8341 cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     8401 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     8461 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     8521 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     8581 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     8641 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     8701 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8761 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8821 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     8881 ctagtccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
     8941 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     9001 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     9061 tgtgaattaa ttcggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     9121 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     9181 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     9241 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     9301 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     9361 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     9421 aagcttgtgt aattgttaat t
//
