LOCUS       sequence-433051-       10978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of rat Kif 1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Montgomery AC, Mendoza CS, Garbouchian A, Quinones GB, Bentley M
  TITLE     Polarized transport requires AP-1-mediated recruitment of KIF13A and
            KIF13B at the trans-Golgi.
  JOURNAL   Mol Biol Cell. 2024 Mar 6:mbcE23100401. doi: 
            10.1091/mbc.E23-10-0401.
  PUBMED    38446634
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2024 Mar 6:mbcE23100401. doi: 10.1091/mbc.E23-10-0401."
COMMENT     Sequence Label: pBa.Halo-KIF1A tail
FEATURES             Location/Qualifiers
     source          1..10978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1239..1261
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1344..2228
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             5727..5738
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6397..6531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6434..6453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6488..6507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6562..6578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6562..6578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6575..6597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6586..6602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6610..6616,6617..6634,6635..6640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6793..6810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6964..7552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7044..7063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7723..8514,8515..8583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8346..8365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8584..8688)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8756..8774
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8812..8834)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8934..8953
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9147..9169
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9161..9178
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9162..9178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 ggggccccag gggggggggg gggggggggg gggggggggg gcggggcggg ggggggggcg
      181 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      241 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      301 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      361 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      421 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      481 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      541 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      601 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      661 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      721 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      781 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      841 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      901 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      961 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1021 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1081 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1141 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1201 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1261 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagctt ggcgcgccta
     1321 ggaccatggc tagcggagga tccgaaatcg gtactggctt tccattcgac ccccattatg
     1381 tggaagtcct gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg
     1441 tgctgttcct gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg
     1501 ttgcaccgac ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac
     1561 cagacctggg ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc
     1621 tgggtctgga agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact
     1681 gggccaagcg caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta
     1741 tcccgacctg ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca
     1801 ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga
     1861 tgggtgtcgt ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga
     1921 atcctgttga ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc
     1981 cagcgaacat cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc
     2041 cgaagctgct gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc
     2101 tggccaaaag cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc
     2161 aagaagacaa cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga
     2221 tttccgggct gtacggcgga ggatccggag gcggctctgg cggagggctg tacaagggcg
     2281 gaggatccgg aggcggctct ggcggagggg atatcactga caccaacact gtgcctggag
     2341 gacccaaatt gaccaatgcc ctggtgggca tgagcccctc gtcttcgctc tcagccctgt
     2401 ccagccgtgc ggcctctgtg tccagtctcc acgagcgcat cttgtttgct ccaggcagcg
     2461 aggaggctat tgaaaggctg aaggaaacag agaagatcat tgctgagctc aatgagacgt
     2521 gggaggagaa gctacggcgg acagaagcca tccggatgga gagggaagcc ctgctggccg
     2581 agatgggtgt ggccatgagg gaagatggtg gcaccttggg agtgttttct cccaaaaaga
     2641 cacctcatct tgtcaacctg aacgaggatc ctttgatgtc tgaatgcctc ctttactaca
     2701 tcaaagatgg cgtcaccaga gtgggcagag aagacgcaga gaggcggcaa gacattgtcc
     2761 tgagtgggca cttcatcaag gaggaacact gcatcttccg gagcgactcc cgaggaggcg
     2821 gagaagctgt ggtgaccctg gagccctgtg aaggagcaga tacctatgtg aacggcaaga
     2881 aagtcactga gcccagcatc ctacggtcag gaaaccgcat catcatggga aaaagccacg
     2941 tgttccggtt taaccacccc gagcaggctc gtcaggagcg tgagcggaca ccttgtgcag
     3001 agacacccgc tgagccagtg gactgggcct ttgcccagag ggagctgctg gagaagcagg
     3061 gcattgacat gaagcaggag atggagcaga ggctccagga gctggaggac cagtaccgga
     3121 gggagcggga ggaggccaca tacctgctgg agcagcagag gctggactat gagagtaagc
     3181 tggaggccct acagaaacag atggactcca ggtactaccc agaggtgaac gaggaggagg
     3241 aagaaccaga ggatgaagtc caatggacgg agcgggagtg tgagttggca ctgtgggcct
     3301 tccggaaatg gaagtggtac cagttcactt ctcttcggga cctgctgtgg ggcaatgcca
     3361 tcttcctgaa ggaggccaat gccatcagtg tggagctaaa gaagaaggtc cagttccagt
     3421 tcgtcctcct cacagatacc ctctactccc cactgccgcc agacctactc cccccagaag
     3481 ctgccaaaga ccgagagaca cgacccttcc ccaggaccat cgtggccgtg gaggtccagg
     3541 accagaagaa tggggccacc cactactgga cgctagagaa actcaggcaa cgcctggacc
     3601 tgatgcggga aatgtatgac cgggctgcag aggttccctc cagcgtcgtt gaggactgtg
     3661 acaatgtggt gactggtgga gaccccttct atgaccgctt cccctggttc cggctggtgg
     3721 gcagggcctt cgtatacctg agcaacttgc tgtaccctgt gcccctggtg caccgcgtgg
     3781 ccatcgtcag cgaaaaggga gaggtgaaag gcttcctccg tgtggcggtg caggccatct
     3841 cagctgatga ggaggctccg gattatggat caggtgtccg tcagtcagga acggctaaaa
     3901 tctccttcga tgaccagcac tttgaaaagt tccagtctga gtcctgccct gtggtgggga
     3961 tgtcccgctc agggacctcc caggaggagc tgcgcattgt agaaggccag ggtcaggggg
     4021 ctgatgctgg gccttcagcc gatgaagtga acaacaacac ttgctcagct gtgcctcctg
     4081 aaggcctcct agatagccca gagaaggctg ccctggatgg acccctagac acagccctgg
     4141 accacctccg tctgggcagt accttcacct tccgggttac agtcctgcag gcgtccagta
     4201 tctctgctga atacgctgac atcttctgcc agttcaactt tatccaccgc cacgatgagg
     4261 ccttctccac tgaacccctg aagaacactg gcagaggacc ccctcttggc ttctaccatg
     4321 tccaaaatat tgcggtagag gtgaccaagt ccttcatcga atacatcaag agccagccca
     4381 ttgtgttcga ggtctttggg cactaccagc agcacccgtt cccacctctt tgcaaggatg
     4441 tcctcagccc cctgagaccc tcacgtcgcc acttccccag ggtcatgccg ttatccaagc
     4501 cagtgcccgc cactaagctc agcacgatga cacggccttc cccaggtccc tgccattgca
     4561 agtatgacct gctggtctac ttcgagatct gcgagctgga agccaatggc gattacatcc
     4621 ctgctgtggt ggaccaccgt ggtggcatgc cgtgcatggg gactttcctt ctccatcagg
     4681 gcatccagag acggataact gtgacactgt tacatgagac aggaagccac atccgctgga
     4741 aggaagtgcg cgagctagtt gtgggtcgca tccgaaacac cccagaaact gacgaagctc
     4801 tgattgaccc caacatcttg tccctcaaca tcctctcatc aggatacgtc catccagccc
     4861 aggatgaccg gcagtttctt gattcggaca tacctaggac cttctaccag tttgaagcag
     4921 cctgggacag ttccatgcat aactctctcc tgctgaatcg tgtcacccct taccgggaga
     4981 aaatctacat gacgctctct gcctacattg agatggagaa ctgtacccag ccagctgtca
     5041 tcaccaagga cttctgcatg gttttctact ctcgggacgc caagctaccc gcctcacgct
     5101 ccattcgcaa cctgtttggc agtggcagcc tgcgggccac agaaggcaac cgtgtgactg
     5161 gtgtgtatga gctcagcctt tgccacgtgg ccgacgcagg cagcccaggg atgcagcgtc
     5221 ggcgccggcg ggtgctggac acatcagtag cctatgtccg gggtgaggag aacctggctg
     5281 gctggaggcc ccgaagtgac agcctgatcc tagatcacca gtgggaactg gagaaactga
     5341 gcctcttaca ggaggtggaa aagaccaggc actacctgct ccttcgggag aagctggaga
     5401 cgacccagcg ccctggccct gaggtgctgt ccccagcctc cagtgaggac tctgagtccc
     5461 gtagttcttc aggtgcctct tccccattgt cagccgaggg tcagccgtca cctctggagg
     5521 ctcccaacga gaggcagagg gagctggctg tcaagtgctt acgactcctc atgcacacat
     5581 tcaacaggga atacacccac agccacgtct gtatcagcgc cagcgagagc aagctctctg
     5641 agatgtctgt caccctgatg cgggacccat ccatgtcccc tctgggtgct gctacactca
     5701 ctccctcctc cacttgcccc tctctgatcg aaggacgata cggtgccact gacgtgagga
     5761 ccccccagcc ctgctcccgg cccgccagcc cagagcccga gctcctgcca gaacttgact
     5821 ctaagaagac tccctcccct gttcgggcaa cagagacaga gaaggagccc cagcgactgc
     5881 tggtccctga catccaagag atccgagtca gcccaatcgt ctccaagaag ggatacctgc
     5941 atttcctgga gccacacacg gctggctggg ccaagcgctt tgtggtggtg aggcgtccct
     6001 acgcctacat gtacaacagt gacaaggaca cggtggagag atttgtgctc aacctctcca
     6061 ctgcgcaggt ggagtacagc gaggaccagc aggccatgct caagacaccc aacacattcg
     6121 ctgtgtgcac ggagcaccga ggcatcctgc tgcaggccaa cagtgacaag gacatgcacg
     6181 actggctgta tgccttcaac ccgctcctgg ctgggaccat ccggtccaag ctctctcgaa
     6241 ggagatctgc gcagatgcgg gtctgagttg aaagccttcc ggaattcatt gatcataatc
     6301 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6361 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccgggtac
     6541 cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8701 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8761 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8821 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8881 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     8941 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9001 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     9121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     9181 caagttggga tctttgcatt ggcccacggc tctcaggatg gggatgctcc ccttcagcac
     9241 ccggttcccc ttggaaactg atggtcctgg ctctgtggca tggcagtggc actgtgagga
     9301 gccccctacc agcagcacac agtggggttg gcactgccac gctccggatg ccgcgctctg
     9361 atccaacccc ataatcaagg gaacccgaat tgccccatca ttgcccccac cacccccatc
     9421 ctgccgggcc ctcacacccc acgctgcctt ctggtgacat tccccagccc aaacccacgg
     9481 cttcatggct accgcggggc atttcccatt gccgccccat tatcagctct gcacacctcc
     9541 cgctgtaccc atgcctcgtg gctgcccttc tttgacgtat aatcttctaa ttaatacccg
     9601 gccttgtcaa agtggagcac aaacgttaat taattcccca gcaggcaggt aattaacagt
     9661 gtgactccct ttttgctgcg agtggggctg atacagagag atgtggcact atggagccca
     9721 cggggtcctg gcactgggtg cccacggagg tccccatgtg ctgcagtgtc accgcctccg
     9781 aggagacagt attgtccctg cggtgtccct gcagctcagc tctgtccaca gggccacctc
     9841 cagtttggag gggacacaat gcagccccga tgcaacccat cctcgcagca tcccagggac
     9901 aaagacccca ctgcaagacc gcacacaggg ctgggtcccg ctcccctaat atctacagtg
     9961 cttcttgcat ggccccttaa tcaatgcagt taatcagcat gcgctcatgc accgctctgg
    10021 agctgcaaag cccctcgcag cgctgctcac caacaccgcg caccgccccg gcccagcctg
    10081 cagcacgcgc tgcaaacagg aaagaaacaa aatattgccc aaatgtaggc aaaggcattc
    10141 ggctgccttg acctccgccg ggccgggccc tgcctgactc agctccttac tcagcgctcg
    10201 cttcctccct ccggctgcca ccgccgcagc gcacaccctg acaaagagtg gcccttaacg
    10261 ggctctgagg tgcacccagc agtgcactca gcagtccaag ggccggcctg gaggtttgca
    10321 ccgctacgtg ctgacattag cattgaactt ggccctgggt agtgctgcag gccgggcggg
    10381 gtgggtgtag agagtgcagc gcgcgttgca cccggtgccc cttcccctcc cttgcatccc
    10441 agcaggctgc accccagcac caggcccgtg catgcatgct cctggtgtta ttgcagcctg
    10501 gtgcatgcat gcgtcttgat ggtgcagcgc tgtgcatgca tcctccttgg tgtgtagcag
    10561 tctagtgcat gcatacccct cggtgttatt gctgctctgt gcacgcacgc tcattgtatc
    10621 acttcatccc agtgcatgca ctcacactgg agcgattgct gctcggtgca cgcacactca
    10681 ttgtatcact gcagctcagt ggctgcacgc acaccggtgt tattgctgct cggtgcgtgc
    10741 atgcacatca gtgtcgctgc agctcagtgc atgcacgctc attgcccatc gctatccctg
    10801 cctctcctgc tggcgctccc cgggaggtga cttcaagggg accgcaggac cacctcgggg
    10861 gtggggggag ggctgcacac gcggaccccg ctccccctcc ccaacaaagc actgtggaat
    10921 caaaaagggg ggagggggga tggaggggcg cgtcacaccc ccgccccaca ccctcacc
//
