LOCUS       sequence-43308-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Iacovino M, Bosnakovski D, Fey H, Rux D, Bajwa G, Mahen E, Mitanoska
            A, Xu Z, Kyba M
  TITLE     Inducible cassette exchange: a rapid and efficient system enabling 
            conditional gene expression in embryonic stem and primary cells.
  JOURNAL   Stem Cells. 2011 Oct;29(10):1580-8.
  PUBMED    22039605
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem 
            Cells."; date: "2011-10"; volume: "29(10)"; pages: "1580-8"
COMMENT     Sequence Label: p2Lox
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(55..71)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(56..72)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    74..107
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    110..143
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(193..692)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(241..260)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     634..657
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctcgccctt gctcaccatg gtggcgttaa ttaagaattc gatatcaagc ttatcgatac
       61 cgtcgacctc gagataactt cgtataaagt atcctatacg aagttatcga taacttcgta
      121 taatgtatgc tatacgaagt tatcgacacc atggtggcct ccagatcctt cgagatctag
      181 atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
      241 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
      301 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
      361 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
      421 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
      481 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
      541 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
      601 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
      661 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttcgaggtcg agatctgtcg
      721 agccatgtga gcaaa
//
