LOCUS       sequence-433099-        6479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang 
            R, Bishop JM
  TITLE     Distinct pathways of genomic progression to benign and malignant 
            tumors of the liver.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
            4.
  PUBMED    17785413
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-09-11"; volume: "104(37)"; pages: 
            "14771-6. Epub 2007 Sep 4"
COMMENT     Sequence Label: pENTR-c-Met
FEATURES             Location/Qualifiers
     source          1..6479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4708..4807)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4919..4938)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4930..5739
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4997..5016)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5829..6417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6318..6337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaattcg cggccgccac catgaaggcc cccgctgtgc ttgcacctgg
      541 catcctcgtg ctcctgttta ccttggtgca gaggagcaat ggggagtgta aagaggcact
      601 agcaaagtcc gagatgaatg tgaatatgaa gtatcagctt cccaacttca ccgcggaaac
      661 acccatccag aatgtcattc tacatgagca tcacattttc cttggtgcca ctaactacat
      721 ttatgtttta aatgaggaag accttcagaa ggttgctgag tacaagactg ggcctgtgct
      781 ggaacaccca gattgtttcc catgtcagga ctgcagcagc aaagccaatt tatcaggagg
      841 tgtttggaaa gataacatca acatggctct agttgtcgac acctactatg atgatcaact
      901 cattagctgt ggcagcgtca acagagggac ctgccagcga catgtctttc cccacaatca
      961 tactgctgac atacagtcgg aggttcactg catattctcc ccacagatag aagagcccag
     1021 ccagtgtcct gactgtgtgg tgagcgccct gggagccaaa gtcctttcat ctgtaaagga
     1081 ccggttcatc aacttctttg taggcaatac cataaattct tcttatttcc cagatcatcc
     1141 attgcattcg atatcagtga gaaggctaaa ggaaacgaaa gatggtttta tgtttttgac
     1201 ggaccagtcc tacattgatg ttttacctga gttcagagat tcttacccca ttaagtatgt
     1261 ccatgccttt gaaagcaaca attttattta cttcttgacg gtccaaaggg aaactctaga
     1321 tgctcagact tttcacacaa gaataatcag gttctgttcc ataaactctg gattgcattc
     1381 ctacatggaa atgcctctgg agtgtattct cacagaaaag agaaaaaaga gatccacaaa
     1441 gaaggaagtg tttaatatac ttcaggctgc gtatgtcagc aagcctgggg cccagcttgc
     1501 tagacaaata ggagccagcc tgaatgatga cattcttttc ggggtgttcg cacaaagcaa
     1561 gccagattct gccgaaccaa tggatcgatc tgccatgtgt gcattcccta tcaaatatgt
     1621 caacgacttc ttcaacaaga tcgtcaacaa aaacaatgtg agatgtctcc agcattttta
     1681 cggacccaat catgagcact gctttaatag gacacttctg agaaattcat caggctgtga
     1741 agcgcgccgt gatgaatatc gaacagagtt taccacagct ttgcagcgcg ttgacttatt
     1801 catgggtcaa ttcagcgaag tcctcttaac atctatatcc accttcatta aaggagacct
     1861 caccatagct aatcttggga catcagaggg tcgcttcatg caggttgtgg tttctcgatc
     1921 aggaccatca acccctcatg tgaattttct cctggactcc catccagtgt ctccagaagt
     1981 gattgtggag catacattaa accaaaatgg ctacacactg gttatcactg ggaagaagat
     2041 cacgaagatc ccattgaatg gcttgggctg cagacatttc cagtcctgca gtcaatgcct
     2101 ctctgcccca ccctttgttc agtgtggctg gtgccacgac aaatgtgtgc gatcggagga
     2161 atgcctgagc gggacatgga ctcaacagat ctgtctgcct gcaatctaca aggttttccc
     2221 aaatagtgca ccccttgaag gagggacaag gctgaccata tgtggctggg actttggatt
     2281 tcggaggaat aataaatttg atttaaagaa aactagagtt ctccttggaa atgagagctg
     2341 caccttgact ttaagtgaga gcacgatgaa tacattgaaa tgcacagttg gtcctgccat
     2401 gaataagcat ttcaatatgt ccataattat ttcaaatggc cacgggacaa cacaatacag
     2461 tacattctcc tatgtggatc ctgtaataac aagtatttcg ccgaaatacg gtcctatggc
     2521 tggtggcact ttacttactt taactggaaa ttacctaaac agtgggaatt ctagacacat
     2581 ttcaattggt ggaaaaacat gtactttaaa aagtgtgtca aacagtattc ttgaatgtta
     2641 taccccagcc caaaccattt caactgagtt tgctgttaaa ttgaaaattg acttagccaa
     2701 ccgagagaca agcatcttca gttaccgtga agatcccatt gtctatgaaa ttcatccaac
     2761 caaatctttt attagtggtg ggagcacaat aacaggtgtt gggaaaaacc tgaattcagt
     2821 tagtgtcccg agaatggtca taaatgtgca tgaagcagga aggaacttta cagtggcatg
     2881 tcaacatcgc tctaattcag agataatctg ttgtaccact ccttccctgc aacagctgaa
     2941 tctgcaactc cccctgaaaa ccaaagcctt tttcatgtta gatgggatcc tttccaaata
     3001 ctttgatctc atttatgtac ataatcctgt gtttaagcct tttgaaaagc cagtgatgat
     3061 ctcaatgggc aatgaaaatg tactggaaat taagggaaat gatattgacc ctgaagcagt
     3121 taaaggtgaa gtgttaaaag ttggaaataa gagctgtgag aatatacact tacattctga
     3181 agccgtttta tgcacggtcc ccaatgacct gctgaaattg aacagcgagc taaatataga
     3241 gtggaagcaa gcaatttctt caaccgtcct tggaaaagta atagttcaac cagatcagaa
     3301 tttcacagga ttgattgctg gtgttgtctc aatatcaaca gcactgttat tactacttgg
     3361 gtttttcctg tggctgaaaa agagaaagca aattaaagat ctgggcagtg aattagttcg
     3421 ctacgatgca agagtacaca ctcctcattt ggataggctt gtaagtgccc gaagtgtaag
     3481 cccaactaca gaaatggttt caaatgaatc tgtagactac cgagctactt ttccagaaga
     3541 tcagtttcct aattcatctc agaacggttc atgccgacaa gtgcagtatc ctctgacaga
     3601 catgtccccc atcctaacta gtggggactc tgatatatcc agtccattac tgcaaaatac
     3661 tgtccacatt gacctcagtg ctctaaatcc agagctggtc caggcagtgc agcatgtagt
     3721 gattgggccc agtagcctga ttgtgcattt caatgaagtc ataggaagag ggcattttgg
     3781 ttgtgtatat catgggactt tgttggacaa tgatggcaag aaaattcact gtgctgtgaa
     3841 atccttgaac agaatcactg acataggaga agtttcccaa tttctgaccg agggaatcat
     3901 catgaaagat tttagtcatc ccaatgtcct ctcgctcctg ggaatctgcc tgcgaagtga
     3961 agggtctccg ctggtggtcc taccatacat gaaacatgga gatcttcgaa atttcattcg
     4021 aaatgagact cataatccaa ctgtaaaaga tcttattggc tttggtcttc aagtagccaa
     4081 aggcatgaaa tatcttgcaa gcaaaaagtt tgtccacaga gacttggctg caagaaactg
     4141 tatgctggat gaaaaattca cagtcaaggt tgctgatttt ggtcttgcca gagacatgta
     4201 tgataaagaa tactatagtg tacacaacaa aacaggtgca aagctgccag tgaagtggat
     4261 ggctttggaa agtctgcaaa ctcaaaagtt taccaccaag tcagatgtgt ggtcctttgg
     4321 cgtgctcctc tgggagctga tgacaagagg agccccacct tatcctgacg taaacacctt
     4381 tgatataact gtttacttgt tgcaagggag aagactccta caacccgaat actgcccaga
     4441 ccccttatat gaagtaatgc taaaatgctg gcaccctaaa gccgaaatgc gcccatcctt
     4501 ttctgaactg gtgtcccgga tatcagcgat cttctctact ttcattgggg agcactatgt
     4561 ccatgtgaac gctacttatg tgaacgtaaa atgtgtcgct ccgtatcctt ctctgttgtc
     4621 atcagaagat aacgctgatg atgaggtgga cacacgacca gcctccttct gggagacatc
     4681 ataggcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtt ggcattataa
     4741 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     4801 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     4861 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     4921 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     4981 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     5041 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     5101 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     5161 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     5221 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     5281 attgttgatg cgctggcagt gtccctgcgc cggttgcatt cgattcctgt ttgtaattgt
     5341 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     5401 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     5461 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     5521 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     5581 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     5641 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     5701 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     5761 taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga aaagatcaaa
     5821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5941 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
