LOCUS       sequence-433113-       10337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PVX vector to express heterologous sequences in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Uranga M, Aragones V, Garcia A, Mirabel S, Gianoglio S, Presa S, 
            Granell A, Pasin F, Daros JA
  TITLE     RNA virus-mediated gene editing for tomato trait breeding.
  JOURNAL   Hortic Res. 2023 Dec 19;11(1):uhad279. doi: 10.1093/hr/uhad279. 
            eCollection 2024 Jan.
  PUBMED    38895601
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2023-12-19"; volume: "11(1):uhad279. doi"; pages: " 
            10.1093/hr/uhad279. eCollection 2024 Jan"
COMMENT     Sequence Label: pLX-PVX
FEATURES             Location/Qualifiers
     source          1..10337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     248..268
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        257..302
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      1010..1019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6007..6028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6043..6048,6049..6066,6067..6073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6081..6097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6086..6108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6105..6121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6105..6121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6117..6389
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWVPNSPYSESYYARSLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAGDA"
     primer_bind     6140..6160
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6142..6160
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6186..6205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6187..6205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6215..6232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6215..6231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6224..6246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(7616..8431)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8423..8442
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(8557..9219)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      9220..9991
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    10211..10235
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    10262..10286
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
       61 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      121 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      181 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      241 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      301 gggaaaacta aaccatacac caccaacaca accaaaccca ccacgcccaa ttgttacaca
      361 cccgcttgaa aaagaaagtt taacaaatgg ccaaggtgcg cgaggtttac caatctttta
      421 cagactccac cacaaaaact ctcatccaag atgaggctta tagaaacatt cgccccatca
      481 tggaaaaaca caaactagct aacccttacg ctcaaacggt tgaagcggct aatgatctag
      541 aggggttcgg catagccacc aatccctata gcattgaatt gcatacacat gcagccgcta
      601 agaccataga gaataaactt ctagaggtgc ttggttccat cctaccacaa gaacctgtta
      661 catttatgtt tcttaaaccc agaaagctaa actacatgag aagaaacccg cggatcaagg
      721 acattttcca aaatgttgcc attgaaccaa gagacgtagc caggtacccc aaggaaacaa
      781 taattgacaa actcacagag atcacaacgg aaacagcata cattagtgac actctgcact
      841 tcttggatcc gagctacata gtggagacat tccaaaactg cccaaaattg caaacattgt
      901 atgcgacctt agttctcccc gttgaggcag cctttaaaat ggaaagcact cacccgaaca
      961 tatacagcct caaatacttc ggagatggtt tccagtatat accaggcaac catggtggcg
     1021 gggcatacca tcatgaattc gctcatctac aatggctcaa agtgggaaag atcaagtgga
     1081 gggaccccaa ggatagcttt ctcggacatc tcaattacac gactgagcag gttgagatgc
     1141 acacagtgac agtacagttg caggaatcgt tcgcggcaaa ccacttgtac tgcatcagga
     1201 gaggagactt gctcacaccg gaggtgcgca ctttcggcca acctgacagg tacgtgattc
     1261 caccacagat cttcctccca aaagttcaca actgcaagaa gccgattctc aagaaaacta
     1321 tgatgcagct cttcttgtat gttaggacag tcaaggtcgc aaaaaattgt gacatttttg
     1381 ccaaagtcag acaattaatt aaatcatctg acttggacaa atactctgct gtggaactgg
     1441 tttacttagt aagctacatg gagttccttg ccgatttaca agctaccacc tgcttctcag
     1501 acacactttc tggtggcttg ctaacaaaga cccttgcacc ggtgagggct tggatacaag
     1561 agaaaaagat gcagctgttt ggtcttgagg actacgcgaa gttagtcaaa gcagttgatt
     1621 tccacccggt ggatttttct ttcaaagtgg aaacttggga cttcagattc caccccttgc
     1681 aagcgtggaa agccttccga ccaagggaag tgtcggatgt agaggaaatg gaaagtttgt
     1741 tctcagatgg ggacttgctt gattgcttca caagaatgcc agcttatgcg gtaaacgcag
     1801 aggaagattt agctgcaatc aggaaaacgc ccgagatgga tgtcggtcaa gaagttaaag
     1861 aacctgcagg agacagaaat caatactcaa accctgcaga aactttcctc aacaagctcc
     1921 acaggaaaca cagtagggag gtgaaacacc aggccgcaaa gaaagctaaa cgcctagctg
     1981 aaatccagga gtcaatgaga gctgaaggtg atgccgaacc aaatgaaata agcgggggca
     2041 tgggggcaat acccagcaac gccgaacttc ctggcacgaa tgatgccaga caagaactca
     2101 cactcccaac cactaaacct gtccctgcaa ggtgggaaga tgcttcattc acagattcta
     2161 gtgtggaaga ggagcaggtt aaaccccttg gaaaagaaac cgttgaaaca gcgacgcaac
     2221 aagtcatcga aggacttcct tggaaacact ggattcctca attaaatgct gttggattca
     2281 aggcgctgga aattcagagg gataggagtg gaacaatgat catgcccatc acagaaatgg
     2341 tctccgggct ggaaaaagag gacttccctg aaggaactcc aaaagagttg gcacgagaat
     2401 tgttcgctat gaacagaagc cctgccacca tccctttgga cctgcttaga gccagagact
     2461 acggcagtga tgtaaagaac aagagaattg gtgccatcac aaagacacag gcaacgagtt
     2521 ggggcgaata cttgacagga aagatagaaa gcttgactga gaggaaagtt gcgacttgtg
     2581 tcattcatgg agctggaggt tctggaaaaa gtcatgccat ccagaaggca ttgagagaaa
     2641 ttggcaaggg ctcggacatc actgtagtcc tgccgaccaa tgaactgcgg ctagattgga
     2701 gtaagaaagt gcctaacact gagccctata tgttcaagac ctatgaaaag gcgttaattg
     2761 ggggaacagg cagcatagtc atctttgacg attactcaaa acttcctccc ggttacatag
     2821 aagccttagt ctgtttctac tctaaaatca agctaatcat tctaacagga gatagcagac
     2881 aaagcgtcta ccatgaaact gctgaggacg cctccatcag gcatttggga ccagcaacag
     2941 agtacttctc aaaatactgc cgatactatc tcaatgccac acaccgcaac aagaaagatc
     3001 ttgcgaacat gcttggtgtc tacagtgaga gaacgggagt caccgaaatc agcatgagcg
     3061 ccgagttctt agaaggaatc ccaactttgg taccctcgga tgagaagaga aagctgtaca
     3121 tgggcaccgg gaggaatgac acgttcacat acgctggatg ccaggggcta actaagccga
     3181 aggtacaaat agtgttggac cacaacaccc aagtgtgtag cgcgaatgtg atgtacacgg
     3241 cactttctag agccaccgat aggattcact tcgtgaacac aagtgcaaat tcctcggcct
     3301 tctgggaaaa gttggacagc accccttacc tcaagacttt cctatcagtg gtgagagaac
     3361 aagcactcag ggagtacgag ccggcagagg cagagccaat tcaagagcct gagccccaga
     3421 cacacatgtg tgtcgagaat gaggagtccg tgctagaaga gtacaaagag gaactcttgg
     3481 aaaagtttga cagagagatc cactctgaat cccatggtca ttcaaactgt gtccaaactg
     3541 aagacacaac cattcagttg ttttcgcatc aacaagcaaa agatgagacc ctcctctggg
     3601 cgactataga tgcgcggctc aagaccagca atcaagagac aaacttccga gaattcctga
     3661 gcaagaagga cattggggac gttctgtttt taaactacca aaaagctatg ggtttaccca
     3721 aagagcgtat tcctttttcc caagaggtct gggaagcttg tgcccacgaa gtacaaagca
     3781 agtacctcag caagtcaaag tgcaacttga tcaatgggac tgtgagacag agcccagact
     3841 tcgatgaaaa taagattatg gtattcctca agtcgcagtg ggtcacaaag gtggaaaaac
     3901 taggtctacc caagattaag ccaggtcaaa ccatagcagc cttttaccag cagactgtga
     3961 tgctttttgg aactatggct aggtacatgc gatggttcag acaggctttc cagccaaaag
     4021 aagtcttcat aaactgtgag accacgccag atgacatgtc tgcatgggcc ttgaacaact
     4081 ggaatttcag cagacctagc ttggctaatg actacacagc tttcgaccag tctcaggatg
     4141 gagccatgtt gcaatttgag gtgctcaaag ccaaacacca ctgcatacca gaggaaatca
     4201 ttcaggcata catagatatt aagactaatg cacagatttt cctaggcacg ttatcaatta
     4261 tgcgcctgac tggtgaaggt cccacttttg atgcaaacac tgagtgcaac atagcttaca
     4321 cccatacaaa gtttgacatc ccagccggaa ctgctcaagt ttatgcagga gacgactccg
     4381 cactggactg tgttccagaa gtgaagcata gtttccacag gcttgaggac aaattactcc
     4441 taaagtcaaa gcctgtaatc acgcagcaaa agaagggcag ttggcctgag ttttgtggtt
     4501 ggctgatcac accaaaaggg gtgatgaaag acccaattaa gctccatgtt agcttaaaat
     4561 tggctgaagc taagggtgaa ctcaagaaat gtcaagattc ctatgaaatt gatctgagtt
     4621 atgcctatga ccacaaggac tctctgcatg acttgttcga tgagaaacag tgtcaggcac
     4681 acacactcac ttgcagaaca ctaatcaagt cagggagagg cactgtctca ctttcccgcc
     4741 tcagaaactt tctttaaccg ttaagttacc ttagagattt gaataagatg gatattctca
     4801 tcagtagttt gaaaagttta ggttattcta ggacttccaa atctttagat tcaggacctt
     4861 tggtagtaca tgcagtagcc ggagccggta agtccacagc cctaaggaag ttgatcctca
     4921 gacacccaac attcaccgtg catacactcg gtgtccctga caaggtgagt atcagaacta
     4981 gaggcataca gaagccagga cctattcctg agggcaactt cgcaatcctc gatgagtata
     5041 ctttggacaa caccacaagg aactcatacc aggcactttt tgctgaccct tatcaggcac
     5101 cggagtttag cctagagccc cacttctact tggaaacatc atttcgagtt ccgaggaaag
     5161 tggcagattt gatagctggc tgtggcttcg atttcgagac caactcaccg gaagaagggc
     5221 acttagagat cactggcata ttcaaagggc ccctactcgg aaaggtgata gccattgatg
     5281 aggagtctga gacaacactg tccaggcatg gtgttgagtt tgttaagccc tgccaagtga
     5341 cgggacttga gttcaaagta gtcactattg tgtctgccgc accaatagag gaaattggcc
     5401 agtccacagc tttctacaac gctatcacca ggtcaaaggg attgacatat gtccgcgcag
     5461 ggccataggc tgaccgctcc ggtcaattct gaaaaagtgt acatagtatt aggtctatca
     5521 tttgctttag tttcaattac ctttctgctt tctagaaata gcttacccca cgtcggtgac
     5581 aacattcaca gcttgccaca cggaggagct tacagagacg gcaccaaagc aatcttgtac
     5641 aactccccaa atctagggtc acgagtgagt ctacacaacg gaaagaacgc agcatttgct
     5701 gccgttttgc tactgacttt gctgatctat ggaagtaaat acatatctca acgcaatcat
     5761 acttgtgctt gtggtaacaa tcatagcagt cattagcact tccttagtga ggactgaacc
     5821 ttgtgtcatc aagattactg gggaatcaat cacagtgttg gcttgcaaac tagatgcaga
     5881 aaccataagg gccattgccg atctcaagcc actctccgtt gaacggttaa gtttccattg
     5941 atactcgaaa gaggtcagca ccagctagca cgcgtgcggg aaagcgggca gtgagcgcaa
     6001 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgctccc
     6061 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6121 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccca
     6181 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6241 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6301 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6361 atggcgaatg ggacgcgggc gacgcgtagg gtttgttaag tttccctttt tactcgaaag
     6421 atgtcaggcc tgttcactat cccggatggg gatttcttta gtacagcccg tgccatagta
     6481 gccagcaatg ctgtcgcaac aaatgaggac ctcagcaaga ttgaggctat ttggaaggac
     6541 atgaaggtgc ccacagacac tatggcacag gctgcttggg acttagtcag acactgtgct
     6601 gatgtaggat catccgctca aacagaaatg atagatacag gtccctattc caacggcatc
     6661 agcagagcta gactggcagc agcaattaaa gaggtgtgca cacttaggca attttgcatg
     6721 aagtatgccc cagtggtatg gaactggatg ttaactaaca acagtccacc tgctaactgg
     6781 caagcacaag gtttcaagcc tgagcacaaa ttcgctgcat tcgacttctt caatggagtc
     6841 accaacccag ctgccatcat gcccaaagag gggctcatcc ggccaccgtc tgaagctgaa
     6901 atgaatgctg cccaaactgc tgcctttgtg aagattacaa aggccagggc acaatccaac
     6961 gactttgcca gcctagatgc agctgtcact cgaggtcgta tcactggaac aacaaccgct
     7021 gaggctgttg tcactctacc accaccataa ctacgtctac ataaccgacg cctaccccag
     7081 tttcatagta ttttctggtt tgattgtatg aataatataa ataaaaaaaa aaaaaaaaaa
     7141 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacgct gaaatcacca gtctctctct
     7201 acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc ccgataaggg
     7261 aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     7321 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     7381 ggtcaggaga cgggacaagg atgcgagcta gcctgcagga attgttgatt ttgtgatgac
     7441 tgatggcagg atatatgcgg ttgtaattca tttttattgt ctaaatttct gtatttgttt
     7501 gtttgttcgg ttgtaaattt ttttggaaga acaagaaaag aaaaaacacc cgttagggtg
     7561 tttttagtta gtgtggcgcg ccgacttgcg acatgcggtc ctttgcaatc aactattaga
     7621 aaaattcatc cagcatcaga tgaaattgca gtttgttcat atccggatta tcaatgccat
     7681 atttctgaaa cagacgtttt tgcaggctcg ggctaaattc gcccaggcag ttccacagaa
     7741 tggccagatc ctgataacga tccgcaatgc ccacacggcc cacatcaatg cagccaatca
     7801 gtttgccttc atcgaaaatc aggttatcca ggctaaaatc gccgtgggtc accacgctat
     7861 ccgggctaaa cggcagcagt ttatgcattt ctttccacac ctgttccacc ggccagccgt
     7921 tacgttcatc atcaaaatcg ctcgcatcca ccaggccgtt gttcatacgg ctctgcgcct
     7981 gggccagacg aaacacacga tcgctgttaa acgggcagtt gcacaccgga atgctatgca
     8041 gacgacgcag aaacacggcc agcgcatcca caatgttttc gccgctatcc ggatattctt
     8101 ccagcacctg aaacgcggtt ttgcccggaa tcgcggtggt cagcagccac gcatcatccg
     8161 gggtgcgaat aaaatgttta atggtcggca gcggcataaa ttcggtcagc cagttcagac
     8221 gcaccatttc atcggtcaca tcgttcgcca cgctgccttt gccatgtttc agaaacagtt
     8281 ccggcgcatc cggtttgcca tacagacgat aaatggtcgc gccgctctga cccacgttat
     8341 cacgcgccca tttatagcca tacagatccg catccatgtt gctgttcaga cgcggacggc
     8401 tacagctcgt ttcacgctga atatggctca taacacccct tgtattactg tttatgtaag
     8461 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     8521 tttgagacac aagatcggat tggcggttat gcggttctac cggcgcggca gcgttacccg
     8581 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     8641 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     8701 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     8761 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     8821 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     8881 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     8941 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     9001 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     9061 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     9121 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     9181 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     9241 gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca
     9301 attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc
     9361 tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt
     9421 taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg
     9481 cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg
     9541 cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc
     9601 cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc
     9661 cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc
     9721 tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc
     9781 gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc
     9841 cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg
     9901 tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg
     9961 ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc
    10021 tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtagggggcc
    10081 atcatcagtt cggtggtctt ccgacgaaca ataaggccgc aaatcgcggc cttttttatt
    10141 gataacaaaa ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa
    10201 gcggcagcgg cggcaggata tattcaattg taaatggctt catgtccggg aaatctactg
    10261 gtggcaggat atattgtggt gtaaacaatg gagaaaaaga ttaattaagt actagtagcc
    10321 tggtcgaaac cgtctca
//
