LOCUS       sequence-43315-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Barg S, Knowles MK, Chen X, Midorikawa M, Almers W
  TITLE     Single secretory granules of live cells recruit syntaxin-1 and 
            synaptosomal associated protein 25 (SNAP-25) in large copy numbers.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20804-9. Epub 2010 Nov
            12.
  PUBMED    21076040
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-11-30"; volume: "107(48)"; pages: 
            "20804-9. Epub 2010 Nov 12"
COMMENT     Sequence Label: peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..396)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(187..208)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     536..555
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     795..816
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tataagctgc ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
       61 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      121 tgatctagag tcgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc
      181 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctaag
      241 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      301 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      361 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      421 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      481 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      541 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      601 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
      661 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      721 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      781 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
//
