LOCUS       sequence-433171-       13623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid harbors the base editor eSCBE3-NG-HF1 along with an 
            sgRNA cloning cassette, facilitating high-efficiency and 
            high-fidelity cytosine base editing at targets bearing NG PAM in 
            Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13623)
  AUTHORS   Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
  TITLE     Engineered cytosine base editor enabling broad-scope and 
            high-fidelity gene editing in Streptomyces.
  JOURNAL   Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
  PUBMED    38971862
REFERENCE   2  (bases 1 to 13623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-7"; volume: "15(1):5687. doi"; pages: " 
            10.1038/s41467-024-49987-3"
COMMENT     Sequence Label: peSCBE3-NG-HF1
FEATURES             Location/Qualifiers
     source          1..13623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..804)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     primer_bind     complement(998..1018)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(998..1016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1045..1063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1236..1304,1305..2096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1454..1473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2756..2775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3009..3026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3240..3374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3264..3283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3318..3337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6592..6893
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     CDS             11700..11951
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     oriT            12314..12423
                     /note="incP origin of transfer"
     CDS             12456..12827
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_RNA        12983..13058
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag
       61 gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg
      121 gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag
      181 ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg
      241 taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc
      301 catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac
      361 ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg
      421 caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg
      481 ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg
      541 tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat
      601 gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc
      661 atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt
      721 aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac
      781 attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa
      841 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
      901 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
      961 acggcatcag ttaccgtgag ctgcaggtcg acggatccct ttagtgaggg ttaattgcgg
     1021 ccgcgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ctgacttccg cgtttccaga
     3061 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     3121 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     3181 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtcag
     3241 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3301 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3361 gcaataaaca agttcccgca cgatcagcgt cccgccgacc aggagcagtg cggccagcac
     3421 tgccgctaac gcctggtcct ggtcccggtc ctggtggtgc atcagtcctc cccgtgatca
     3481 cttcggcacc caccgtagtg atcacccccg acagcggatc aaggggtttg cgggtcccgg
     3541 tcggcgccgg gcgggggagg caggagccgc cgacgctgcc tctgggacgg gccggacggc
     3601 agggggaccg gcggccgggc gagctgcagc cgggggtccg gcagggccgg agcgggcgga
     3661 accgtgctct gacctgcggc ccgagtttcg tcacgtgacg gaatggaagg ctgctgcatt
     3721 tcgtcacgtg acgtatctcg gcgagcgact gccgacgcca cggcggacac gatcgcctcg
     3781 cgctggcgcc gggcctcgta cgcccgctgg cggcaggagc ggcggcagta gtcccggctc
     3841 cggccgacgc cggattgctt gatctccgag ccgcaccagg cgcagagctt cgcgccgtcg
     3901 gcgtccctgg gggtggtggt gctcatggcc gacgaccgta cgcggcacgt ctcgtagcga
     3961 ggcgagtcgg gcgcgaggta ccgcctgcac gaagtgccgg cggggccgac cccgggcgag
     4021 taatcccagg attactcccg cggcttcgac cccggccgcc gtcgccgcgt acgtcaccga
     4081 cccccgccgt acgtcaccgg gatgacgtac ggcggggggg agcgagttag tgcgaagtgg
     4141 gcccacttgc gagccgggcg atgtgccggg cggcccgctc ctggcggtcg tcggcgtcgt
     4201 cgtcctggtc gtcgtcctgc tctcgccgtc ggcgtgcagt tgcttcctcg cggcgctggg
     4261 cgagggcggc gagcatgtcg gcgtacgcct cggccacctc ccccgccgtg agcaccacca
     4321 ctgtgtcggc cgcgtcggcc agcgccagga cctcccgcac ccgttcgccc acggccgccg
     4381 aatcctcgtt gccgtccttg ccttcggcgg cccgggtcgc ctcgaggtcg agggcgcggc
     4441 gggtgaccgc gtgccatccg tcctcggtca cggcgacccc ggcccgcagc tccccgccgt
     4501 cggcgtcggc cgccaggagc agatcgaggt cgtcggcctc ggtgtcgccg ccgtcgagcc
     4561 cgagcatctg ccgcaggtag cgggtccatt cgatggcccg gcgtccccgg gttgcccgct
     4621 cgtactcgtg ccagcgcgag aggttccact ccagcgagcc gaccccggcg gcgtcgtcct
     4681 cggtcatgcc gccggtcagg tccccgatcc gtccgaggag ttcgaacggg gcgacgttcc
     4741 cgccggtcgc cgtcttgagg tcggcgcggg cgagttcgag ggcgggcgcc ttcccgtcct
     4801 gggtcttggc gatgtactcg gcgaggtcgt tggcgtcgcg ctcggtctcc agccgcttga
     4861 agtcgacgcc gtgccggtcg tcgggcgtga aggcggggtt gaccttgcgc agggcggcgg
     4921 tccacacgga ccgccagtgc ccctgccact cgtcgagcgc ggcgccggtc ggctcgaagg
     4981 tggcgacgat ctgcttcgcg gaccgctccc cctcggtccg gccgccgacc aggacgatcg
     5041 cgtggatgtg cgggtgccag ccgttgatct gccccacggt gacttcggtc gcgcggatca
     5101 tgccgacgta cccgatccgg tctcggatgc cctcgcggtc ggcggcccgg tgcccgtcct
     5161 tggcccggcg tccggcccac gtgccgcccg tgatcagtcg ctggtaggcg cccggccgcc
     5221 gggggctgtc cggcgtcttc cgggtgccct ggagggcgtc catgaggtcc gcgagccggt
     5281 ccgtgtgccc atggcgggcc gtgaaggtga ccaggtaggc ggtccccccg cgcttgatcc
     5341 actcgaccac ggcggcggtg atctcctcgg cccgcttgtg ccggatcgtg gcggcgcaga
     5401 ccgggcagag ccagatccgc ccgcaccgca tcaggcccag gaccacggac gttccggccg
     5461 ccgtctgggc gacgatcacg ccggaggcag ggtccatcag ggcgcggccg cagcccttgc
     5521 acgcggcgtc cccgctgatc cgccacagcg tccggcggcg gctgtaccgg gcggctttcc
     5581 gcagtcgggc agcgtcgctc cgcgacgtgc ttcctacttc cgagaggctg tcgcctctcg
     5641 ggctctcccc atccaccccg tccggagaaa ccgcaggtcg gaggggtgcg ggaaactctg
     5701 ttgtttcttt cccaaggtgt tcgcttttgc ctcgggcggc atctcgcgtc acacgcgcga
     5761 tcgcccgctt cgctgccatc cggcagcggt ctgagcagta gatacgcggc cgtttgcccg
     5821 gtgtgtgggc aattgcggtc ccgcagtggc agcggggccc ggcgggccga tctggcaatg
     5881 cctcggcatc gctccgtact ctgggcacga gcaacgttcc tgtctcgccc ggctaagggg
     5941 cgcgagtctg ggagcggacg ggtcggaggt gcgaagtccg gcccgttgct ctttggtctg
     6001 gtgggaatcc tggcaccaat cgggccagag gttccctccg ccactcccga cgccccttgg
     6061 ggctggtgtg acttggaggg ccgaagagag ccccgccggt gatccggcgg ggctttgacg
     6121 tgcggtcagt gcgtgtgtcg gcgagcgatg gccacgaggc cctggaagcc gagcggtccg
     6181 gcgaagtcgg cccagtcgca accgggctca gcgcagtggg cggaccagcc accgccgttg
     6241 gggtcctgga ccaggttcac ggtcccctcg gtcaggcgtc cgtcgaagtc ggtcatggtc
     6301 ggtctcctgg tgggtggggg cggggcgcca gcacgaagtg ccggcgcccc gcgggggttg
     6361 gtcgggtcag gcgccgaacc ggcgggcggc ggcggcgacc aggccgtcgg cggcggccat
     6421 ggcgcggtcg cggtcggtgg tgagggcggt gcggtcggcg gcggcccagt cctgctctca
     6481 cgcaacgtct acgtggacgc tcagggcgac acgatcgagg tcggggagtc cgtcgctccg
     6541 gcgggccgct ggcgcgtcca ccgggactga tcaaggcgaa tacttcatat ggccctgcag
     6601 gcggaagtca ggtagacacg acttccgcta gtccttgcaa ggtctgctga cgtgaggcgg
     6661 ggcggtcgtt tttgaccgcc ctgccttcgt catgtaggct cgctcgctgt gcctggcgtg
     6721 tcatcagacg cccaggtccc ggtgccgtga ggcccgggcc atcgagccgg tggtacgtgg
     6781 ctgcggtccc cttgtgaggg ctgcgcgccg tgtgctgtcc ggcgcgcaca gccttgaatc
     6841 cacccgcggg ggccggccgg tctccgtgag ctcgagtaga cgacggagac gtaatggagg
     6901 cctcccccgc ctccggcccc cgccacctga tggacccgca catcttcacc tccaacttca
     6961 acaacggcat cggccgccac aagacctacc tgtgctacga ggtcgagcgg ctggacaacg
     7021 gcacctccgt caagatggac cagcaccggg gcttcctgca caaccaggcc aagaacctgc
     7081 tgtgcggctt ctacggccgc cacgccgagc tgcggttcct ggacctggtc ccctccctgc
     7141 agctggaccc cgcccagatc taccgcgtca cctggttcat ctcctggtcc ccgtgcttct
     7201 cctggggctg cgccggcgag gtccgggcct tcctgcagga gaacacccac gtccgcctgc
     7261 ggatcttcgc cgcccgcatc ttcgactacg accccctgta caaggaagcc ctgcagatgc
     7321 tgcgggacgc cggcgcccag gtctccatca tgacctacga cgagttcaag cactgctggg
     7381 acaccttcgt ggaccaccag ggctgcccct tccagccctg ggacggcctg gacgagcact
     7441 cccaggccct gtccggccgc ctgcgggcca tcctgcagaa ccagggcaac tccggctccg
     7501 agaccccggg cacctccgag tccgccaccc ccgagtcctc cggctcggag accccgggca
     7561 cctcggagtc cgccaccccg gagtccgaca agaagtacag catcggcctg gccatcggca
     7621 ccaacagcgt gggctgggcg gtcatcaccg acgagtacaa ggtcccctcc aagaagttca
     7681 aggtcctggg caacaccgac cggcactcga tcaagaagaa cctgatcggc gccctgctct
     7741 tcgacagcgg cgagaccgcc gaggcgaccc gcctgaagcg gaccgcgcgc cggcgctaca
     7801 cccggcgcaa gaaccgcatc tgctacctcc aggaaatctt ctccaacgag atggccaagg
     7861 tcgacgactc gttcttccac cggctcgagg agagcttcct ggtggaggag gacaagaagc
     7921 acgagcgcca cccgatcttc ggcaacatcg tcgacgaggt ggcctaccac gagaagtacc
     7981 ccaccatcta ccacctccgc aagaagctgg tcgactcgac cgacaaggcg gacctgcggc
     8041 tcatctacct ggccctcgcg cacatgatca agttccgcgg ccacttcctc atcgagggcg
     8101 acctgaaccc ggacaactcc gacgtcgaca agctcttcat ccagctggtg cagacctaca
     8161 accagctgtt cgaggagaac cccatcaacg ccagcggcgt cgacgccaag gcgatcctct
     8221 ccgcgcgcct gagcaagtcc cggcgcctgg agaacctcat cgcccagctg ccgggcgaga
     8281 agaagaacgg cctcttcggc aacctgatcg cgctgtcgct cggcctgacc cccaacttca
     8341 agagcaactt cgacctggcc gaggacgcga agctccagct gtccaaggac acctacgacg
     8401 acgacctgga caacctgctc gcccagatcg gcgaccagta cgcggacctc ttcctggccg
     8461 cgaagaacct ctcggacgcc atcctgctca gcgacatcct gcgggtcaac accgagatca
     8521 ccaaggcccc gctgtcggcg agcatgatca agcggtacga cgagcaccac caggacctga
     8581 ccctgctcaa ggccctcgtg cgccagcagc tgcccgagaa gtacaaggaa atcttcttcg
     8641 accagtccaa gaacggctac gccggctaca tcgacggcgg cgcgtcgcag gaggagttct
     8701 acaagttcat caagccgatc ctggagaaga tggacggcac cgaggagctg ctcgtcaagc
     8761 tgaaccgcga ggacctgctc cgcaagcagc ggaccttcga caacggctcc atcccgcacc
     8821 agatccacct gggcgagctg cacgccatcc tccggcgcca ggaggacttc taccccttcc
     8881 tgaaggacaa ccgcgagaag atcgagaaga tcctgacctt ccgcatcccg tactacgtcg
     8941 gccccctggc ccgcggcaac tcccggttcg cgtggatgac ccggaagtcg gaggagacca
     9001 tcaccccgtg gaacttcgag gaggtcgtgg acaagggcgc ctccgcccag tcgttcatcg
     9061 agcgcatgac cgccttcgac aagaacctcc cgaacgagaa ggtcctgccc aagcactccc
     9121 tgctctacga gtacttcacc gtgtacaacg agctgaccaa ggtcaagtac gtgaccgagg
     9181 gcatgcggaa gccggccttc ctgtcgggcg agcagaagaa ggcgatcgtc gacctgctct
     9241 tcaagaccaa ccgcaaggtc accgtgaagc agctgaagga ggactacttc aagaagatcg
     9301 agtgcttcga ctccgtcgag atcagcggcg tggaggaccg cttcaacgcc tccctgggca
     9361 cctaccacga cctgctcaag atcatcaagg acaaggactt cctcgacaac gaggagaacg
     9421 aggacatcct ggaggacatc gtcctcaccc tgaccctctt cgaggaccgc gagatgatcg
     9481 aggagcggct caagacctac gcccacctgt tcgacgacaa ggtgatgaag cagctgaagc
     9541 ggcgccggta caccggctgg ggcgccctct cccggaagct gatcaacggc atccgggaca
     9601 agcagagcgg caagaccatc ctggacttcc tcaagtccga cggcttcgcc aaccgcaact
     9661 tcatggcgct catccacgac gacagcctga ccttcaagga ggacatccag aaggcccagg
     9721 tctcgggcca gggcgacagc ctccacgagc acatcgccaa cctggcgggc tccccggcga
     9781 tcaagaaggg catcctccag accgtcaagg tcgtggacga gctggtcaag gtgatgggcc
     9841 gccacaagcc cgagaacatc gtgatcgaga tggcccggga gaaccagacc acccagaagg
     9901 gccagaagaa ctcgcgcgag cggatgaagc ggatcgagga gggcatcaag gagctgggca
     9961 gccagatcct gaaggagcac ccggtcgaga acacccagct ccagaacgag aagctgtacc
    10021 tctactacct ccagaacggc cgcgacatgt acgtggacca ggagctggac atcaaccggc
    10081 tgtccgacta cgacgtcgac cacatcgtgc cgcagtcctt cctgaaggac gactcgatcg
    10141 acaacaaggt cctgacccgc tcggacaaga accggggcaa gtccgacaac gtgccctcgg
    10201 aggaggtcgt gaagaagatg aagaactact ggcgccagct gctcaacgcc aagctcatca
    10261 cccagcgcaa gttcgacaac ctgaccaagg ccgagcgggg cggcctgagc gagctggaca
    10321 aggcgggctt catcaagcgc cagctggtcg agacccgggc gatcaccaag cacgtggccc
    10381 agatcctgga ctcccggatg aacaccaagt acgacgagaa cgacaagctg atccgcgagg
    10441 tcaaggtgat caccctcaag agcaagctgg tctccgactt ccgcaaggac ttccagttct
    10501 acaaggtccg ggagatcaac aactaccacc acgcccacga cgcgtacctg aacgccgtcg
    10561 tgggcaccgc gctgatcaag aagtacccga agctggagtc cgagttcgtc tacggcgact
    10621 acaaggtcta cgacgtgcgc aagatgatcg ccaagagcga gcaggagatc ggcaaggcca
    10681 ccgcgaagta cttcttctac tccaacatca tgaacttctt caagaccgag atcaccctgg
    10741 ccaacggcga gatccgcaag cggcccctga tcgagaccaa cggcgagacc ggcgagatcg
    10801 tctgggacaa gggccgcgac ttcgccaccg tccggaaggt gctgtcgatg ccgcaggtca
    10861 acatcgtgaa gaagaccgag gtgcagaccg gcggcttcag caaggagtcc atccggccca
    10921 agcgcaacag cgacaagctg atcgcccgga agaaggactg ggacccgaag aagtacggcg
    10981 gcttcgtcag ccccaccgtc gcctactccg tgctggtcgt ggcgaaggtc gagaagggca
    11041 agagcaagaa gctgaagtcc gtgaaggagc tgctcggcat caccatcatg gagcgctcct
    11101 cgttcgagaa gaacccgatc gacttcctgg aggccaaggg ctacaaggag gtcaagaagg
    11161 acctcatcat caagctgccc aagtacagcc tgttcgagct ggagaacggc cgcaagcgga
    11221 tgctcgcctc cgcgcggttc ctgcaaaagg gcaacgagct ggccctcccg tcgaagtacg
    11281 tcaacttcct gtacctcgcg tcccactacg agaagctgaa gggctcgccc gaggacaacg
    11341 agcagaagca gctcttcgtg gagcagcaca agcactacct ggacgagatc atcgagcaga
    11401 tcagcgagtt cagcaagcgc gtcatcctgg ccgacgcgaa cctcgacaag gtgctgtccg
    11461 cctacaacaa gcaccgcgac aagccgatcc gggagcaggc ggagaacatc atccacctgt
    11521 tcaccctcac caacctgggt gccccgcggg ccttcaagta cttcgacacc accatcgacc
    11581 gcaaggtcta ccgctccacc aaggaggtcc tcgacgcgac cctgatccac cagagcatca
    11641 ccggcctgta cgagacccgc atcgacctga gccagctggg cggcgactcg ggcggctcca
    11701 ccaacctgtc ggacatcatc gagaaggaga ccggcaagca gctggtgatc caggagtcca
    11761 tcctgatgct gccggaggaa gtggaggaag tgatcggcaa caagccggag tcggacatcc
    11821 tggtgcacac cgcctacgac gagtcgaccg acgagaacgt catgctgctg acctcggacg
    11881 ccccggagta caagccgtgg gccctggtca tccaggactc caacggcgag aacaagatca
    11941 agatgctgtg agaattcaga tctacgcgtt ccccgcaaaa gcggcctttg actccctgca
    12001 agcctcagcg accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc
    12061 aactatcggt atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac
    12121 aattaaaggc tccttttgga gccttttttt aagcttgcat gcctgcaggt cgacggatct
    12181 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
    12241 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
    12301 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
    12361 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
    12421 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
    12481 ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg
    12541 aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct
    12601 acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg
    12661 acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc
    12721 ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc
    12781 ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc
    12841 taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag
    12901 agcgacttcg cggagctggt gaagtacatc accgacgagc aaggcaagac cgatccccgg
    12961 ggacctgcag gtcgactcta gagttttaga gctagaaata gcaagttaaa ataaggctag
    13021 tccgttatca acttgaaaaa gtggcaccga gtcggtgcta aaaaaaaacc ccgcccctga
    13081 cagggcgggg ttttttttgt agcctcagga gttgtagcgt gcacgcggtc gatcttgacg
    13141 gctggcgaga ggtgcgggga ggatctgacc gacgcggtcc acacgtggca ccgcgatgct
    13201 gttgtgggca caatcgtgcc ggttggtagg atccagctag ctatttaccc gcaggacata
    13261 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
    13321 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
    13381 ttcaggcttt ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc
    13441 gccgtgccaa tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg
    13501 cattgagcgt cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg
    13561 tgttgcccca gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg
    13621 agc
//
