LOCUS       sequence-433176-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     115..134
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     140..159
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      245..833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     734..753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     987..1004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1102..1119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1103..1119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  1528..2295
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    2352..2368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        2535..2914
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2915..3118
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3069..3089
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3115..3139
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3349..3367
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3349..3366
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3380..3399
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7318..7362
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7363..7380
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7520..7537)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7526..7637
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7755..7775)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7760..8089
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7940..8075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8002..8021
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8137..8184
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8203..8802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8203..8222)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8699..8719
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8932..9053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8969..8988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9023..9042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9106..9897,9898..9966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9729..9748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9967..9985,1..73))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
       61 aaaaataaac aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg
      121 agcccattta tacctgaata tggctcataa caccccttgc tcatgaccaa aatcccttaa
      181 cgtgagttac gcgcgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttagcccacc
      421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      901 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      961 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaggcgaga
     1021 gtagggaact gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt
     1081 ttctacaaac tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg
     1141 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
     1201 ttttttatgg ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca
     1261 ctttgcttga tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta
     1321 aataagatac gttgcttttt cgattgatga acacctataa ttaaactatt catctattat
     1381 ttatgatttt ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc
     1441 tcgaaaataa taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat
     1501 acaaaatata atacaaacta taagatgggt gatgctgcca acttactgat ttagtgtatg
     1561 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     1621 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
     1681 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
     1741 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
     1801 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
     1861 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
     1921 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
     1981 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
     2041 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
     2101 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
     2161 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
     2221 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
     2281 gttggagtca ttacctataa gatgttatca gtatttatta tcatttagaa taaattttgt
     2341 gtcgccctta attgtgagcg gataacaatt acgagcttca tgcacagtgg cgttgactca
     2401 atattggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
     2461 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2521 atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2581 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2641 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2701 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2761 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2821 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2881 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2941 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3001 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3061 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3121 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3181 acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg
     3241 tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct tggcttcgtt
     3301 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     3361 ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     3421 cacctcggtt ctatcgaaaa cgcgcctgct agcgccacca tgaagaccat cattgccctg
     3481 agctacatct tctgcctggt gttcgctgcg gccgcccaat ttgagctgga aatcctgagc
     3541 atgcagaacg tgaacggcga gctgcagaat ggcaattgct gtggcggagc cagaaatccc
     3601 ggcgacagaa agtgcaccag agatgagtgc gacacctact tcaaagtgtg cctgaaagaa
     3661 taccagagcc gcgtgacagc cggcggacct tgttcttttg gcagcggctc tacacctgtg
     3721 atcggcggca acaccttcaa cctgaaggcc agcagaggca acgaccggaa cagaatcgtg
     3781 ctgcccttca gcttcgcctg gcctagaagc tacaccctgc tggtggaagc ctgggacagc
     3841 agcaatgata ccgtgcagcc cgacagcatc atcgagaagg cctctcacag cggcatgatc
     3901 aaccccagca gacagtggca gaccctgaag cagaataccg gcgtggccca cttcgagtac
     3961 cagatcagag tgacctgcga cgactactac tacggcttcg gctgcaacaa gttctgcaga
     4021 ccccgggacg atttcttcgg ccactatgcc tgcgaccaga acggcaacaa gacctgcatg
     4081 gaaggctgga tgggccccga gtgcaataga gccatctgca gacagggctg tagccccaag
     4141 cacggcagct gtaaactgcc cggcgattgc agatgccagt atggctggca gggcctgtac
     4201 tgcgacaagt gcattcctca tcctggctgc gtgcacggaa tctgcaatga gccttggcag
     4261 tgcctgtgcg agacaaactg gggaggccag ctgtgcgaca aggacctgaa ttactgcggc
     4321 acccaccagc cttgcctgaa tggcggcaca tgtagcaaca caggccccga caagtaccag
     4381 tgctcttgcc ctgagggcta ctctggcccc aattgcgaga ttgccgaaca cgcctgtctg
     4441 agcgacccct gccacaatag aggcagctgc aaagagacaa gcctgggctt cgagtgcgag
     4501 tgttctcctg gatggacagg ccctacctgc agcaccaaca tcgacgactg cagccctaac
     4561 aactgcagcc acggcggaac ctgtcaggac ctggtcaacg gctttaagtg cgtgtgccct
     4621 cctcagtgga ccggcaaaac atgccagctg gacgccaacg agtgcgaagc caagccttgt
     4681 gtgaacgcca agagctgcaa gaacctgatc gccagctact actgcgactg cctgccagga
     4741 tggatgggac agaactgcga catcaacatc aacgattgcc tgggccagtg ccagaacgac
     4801 gccagctgta gagatctggt caatggctac cggtgcatct gcccacctgg atatgcaggc
     4861 gatcactgcg agcgggacat tgacgagtgt gccagcaatc cctgtctgaa cggcggccac
     4921 tgtcagaacg agatcaaccg gttccagtgt ctgtgcccca ccggctttag cggcaatctg
     4981 tgtcagctgg acatcgacta ttgcgagccc aatccatgcc agaatggcgc ccagtgctac
     5041 aacagagcca gcgactactt ctgcaagtgc cccgaggatt acgagggcaa gaactgctcc
     5101 cacctgaagg accactgccg gaccacacct tgcgaagtga tcgatagctg caccgtggct
     5161 atggccagca acgatacacc agaaggcgtg cggtacatca gcagcaacgt gtgtggacct
     5221 cacggcaagt gcaagtctca gagcggcggc aagttcacct gtgactgtaa caagggcttc
     5281 accggcacct actgccacga gaacatcaat gactgcgaga gcaacccctg cagaaacggc
     5341 ggcacttgta tcgacggcgt gaacagctac aagtgtatct gcagcgacgg atgggagggc
     5401 gcctactgcg agactaatat caacgactgc tctcagaacc cttgccacaa cggcgggacc
     5461 tgcagagatc tcgtgaacga cttctactgc gattgcaaga acggctggaa gggcaagacc
     5521 tgtcacagca gagatagcca gtgcgacgag gccacctgta acaatggcgg aacttgctac
     5581 gatgagggcg acgccttcaa gtgcatgtgt cctggtggat gggaaggcac cacatgcaat
     5641 atcgcccgga acagcagctg tctgcctaat ccatgtcata acggtggcac ctgtgtcgtg
     5701 aatggcgaga gctttacctg cgtgtgcaaa gaaggctggg agggacctat ctgcgcccag
     5761 aacaccaacg actgtagccc acatccttgc tacaactccg gcacatgcgt ggacggcgac
     5821 aactggtaca gatgcgaatg cgcccctggc ttcgccggac ctgattgccg gatcaatatc
     5881 aatgagtgtc agagcagccc ttgcgccttc ggcgctactt gtgtggatga gatcaacggc
     5941 taccgctgcg tgtgtccacc aggccatagc ggagccaagt gtcaagaagt gtccggcaga
     6001 ccttgcatca ccatgggcag cgttatcccc gatggcgcca agtgggacga cgattgcaat
     6061 acctgccagt gcctcaacgg cagaatcgcc tgctccaaag tttggtgcgg ccccagacct
     6121 tgtctgctgc acaagggaca cagcgagtgt cctagcggcc agagctgtat ccccatcctg
     6181 gacgatcagt gcttcgtgca cccttgtaca ggcgtgggcg agtgtagaag cagctctctg
     6241 cagcccgtga aaacaaagtg taccagcgat agctactacc aggacaactg cgccaacatc
     6301 accttcacgt tcaacaaaga aatgatgagc cctggcctga ccaccgagca catctgtagc
     6361 gagctgagaa acctgaacat cctgaagaac gtgtccgccg agtacagcat ctatatcgcc
     6421 tgcgagcctt ctcctagcgc caacaacgaa atccacgtgg ccatcagcgc cgaggacatc
     6481 agagatgacg gcaaccccat caaagagatc accgacaaga tcatcgacct ggtgtccaag
     6541 cgcgacggca actcttctct gattgctgcc gtcgctgaag tgcgggtcca gagaaggccc
     6601 ctgaagaata gaaccgatgg cgcgccgggc ggcgacaaga cccacacctg ccccccctgc
     6661 ccagctccag aactgctggg agggccttcc gtgttcctgt tcccccccaa gcccaaggac
     6721 accctgatga tcagcaggac ccccgaggtg acctgcgtgg tggtggccgt gtcccacgag
     6781 gacccagagg tgaagttcaa ctggtacgtg gacggcgtgg aggtgcacaa cgccaagacc
     6841 aagcccagag aggagcagta caacagcacc tacagggtgg tgtccgtgct gaccgtgctg
     6901 caccaggact ggctgaacgg caaagaatac aagtgcaaag tctccaacaa ggccctggct
     6961 gccccaatcg aaaagacaat cagcaaggcc aagggccagc cacgggagcc ccaggtgtac
     7021 accctgcccc ccagccggga ggagatgacc aagaaccagg tgtccctgac ctgtctggtg
     7081 aagggcttct accccagcga tatcgccgtg gagtgggaga gcaacggcca gcccgagaac
     7141 aactacaaga ccaccccccc agtgctggac agcgacggca gcttcttcct gtacagcaag
     7201 ctgaccgtgg acaagtccag gtggcagcag ggcaacgtgt tcagctgcag cgtgatgcac
     7261 gaggccctgc acaaccacta cacccagaag tccctgagcc tgagccccgg cggcagcggt
     7321 cttaatgata ttttcgaggc tcagaagatt gagtggcatg aacaccacca ccatcaccac
     7381 taaggccggc ctgtagtcac tggcacaacg cgttacccgg taagccaatc gggtatacac
     7441 ggtcgtcata ctgcagacag ggttcttcta ctttgcaaga tagtcttgag tagtaaaata
     7501 aatagataga gaaaatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7561 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7621 aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt gatttataag
     7681 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7741 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7801 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7861 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7921 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7981 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8041 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8101 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8161 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
     8221 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8281 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8341 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8401 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8461 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8521 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8581 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8641 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc
     8701 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8761 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct acgagatttc
     8821 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8881 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8941 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9001 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9061 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc aatgcttaat
     9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat
     9241 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag
     9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc
     9421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9961 actcatattc ttcctttttc aatat
//
