LOCUS       sequence-433185-        9134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding Grb2-eDHFR(69K6) chimera fused to iRFP713.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K
  TITLE     Front-biased activation of Ras-Rab5-Rac1 loop coordinates collective
            cell migration.
  JOURNAL   J Cell Sci. 2025 Jul 16:jcs.263779. doi: 10.1242/jcs.263779.
  PUBMED    40667649
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Jul 16:jcs.263779. doi: 10.1242/jcs.263779."
COMMENT     Sequence Label: pPBpuro-chGrb2/eDHFR(69K6)-iRFP713
FEATURES             Location/Qualifiers
     source          1..9134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5040..5589
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5184..5201)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5411..5430
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5592..6191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5592..5611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6088..6108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6218..6235)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6224..6448
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6495..6511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6519..6535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6543..6549,6550..6567,6568..6573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6588..6609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6843..6905
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6946..6966)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6946..6964)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6985..7001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7009..7025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7033..7039,7040..7057,7058..7063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7078..7099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7216..7233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7387..7975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7467..7486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8146..8937,8938..9006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8769..8788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9007..9111)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaatta accatgggcg
     2761 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2821 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2881 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2941 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcatag
     3001 tagctagcag ttgctcgacc accatggaag ccatcgccaa atatgacttc aaagctactg
     3061 ctgacgatga gctgagcttc aagagggggg acatccttaa ggttttgaat gaagagtgtg
     3121 atcagaactg gtataaggca gaactcaatg ggaaagatgg cttcatcccc aagaattaca
     3181 tagaaatgaa accacatccg tggcggcccg ggtacaagtc tgggtccgca gccagtagcg
     3241 ccggaagtat gatcagtctg attgcggcgt tagcggtaga tcgcgttatc ggcatggaaa
     3301 acgccatgcc gtggaacctg cctgccgatc tcgcctggtt taaacgcaac accttaaata
     3361 aacccgtgat tatgggccgc catacctggg aatcaatcgg tcgtccgttg ccaggacgca
     3421 aaaatattat cctcagcagt caaccgggta cggacaaaaa aaagaaaaag aaagatcgcg
     3481 taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta ccagaaatca
     3541 tggtgattgg cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc
     3601 tgacgcatat cgacgcagaa gtggaaggcg acacccattt cccggattac gagccggatg
     3661 actgggaatc ggtattcagc gaattccacg atgctgatgc gcagaactct cacagctatt
     3721 gctttgagat tctggagcgg cggctcgagt cgggtggcgg cggatcttca gctggtgctc
     3781 aggtgccaca gcagccaacg gaagtccagg cactctttga ctttgacccc caggaggatg
     3841 gtgagctggg ctttcgccga ggagacttca ttcatgtcat ggataactca gatcccaatt
     3901 ggtggaaagg ggcctgccat gggcagaccg gcatgtttcc ccgcaattat gtcaccccag
     3961 tgaaccggaa cgtcggcggc cgctctggtg ctggtagtgg cgctggttcc ggtatggccg
     4021 aagggagcgt ggcaagacag cccgatctgc tgacatgcga cgacgaacca atccacattc
     4081 caggagctat ccagcctcat ggactgctgc tggctctggc cgctgacatg actatcgtgg
     4141 caggctctga taacctgcca gagctgaccg ggctggctat cggagcactg attggccggt
     4201 ccgcagccga cgtgttcgat tctgagactc acaatagact gaccatcgcc ctggctgaac
     4261 ctggggctgc agtgggagcc ccaattaccg tcggcttcac aatgcgaaag gacgctggct
     4321 ttatcgggtc ctggcaccgc catgatcagc tgatttttct ggagctggaa ccacctcaga
     4381 gagacgtggc agagccacag gctttctttc ggagaacaaa cagcgccatc aggcgcctgc
     4441 aggccgctga gactctggaa tccgcttgcg cagccgctgc acaggaagtg aggaagatca
     4501 ccgggttcga tcgcgtcatg atctacagat tcgcctcaga ctttagcgga gaagtgatcg
     4561 cagaagatcg ctgtgccgag gtcgaaagta aactgggcct gcattaccca gcctcaaccg
     4621 tgcctgcaca ggcacgacgg ctgtatacaa tcaaccccgt gcggatcatt cccgacatta
     4681 attacagacc agtgcccgtc acacctgacc tgaacccagt gactgggagg cccatcgatc
     4741 tgagcttcgc cattctgcgc agcgtgtccc ctgtccacct ggagtttatg aggaatatcg
     4801 gaatgcatgg caccatgtct atcagtattc tgagaggcga aaggctgtgg gggctgatcg
     4861 tgtgccacca tcggacacct tactatgtgg acctggacgg caggcaggct tgtgaactgg
     4921 tggctcaggt gctggcttgg cagatcggcg tgatggaaga gtagggccgc tctagagtca
     4981 actaacttaa gctagcaacg gtttccctct agcgggatca attccgcccc ccccccctaa
     5041 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5101 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5161 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5221 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5281 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5341 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5401 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5461 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5521 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5581 acgataatac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5641 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5701 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5761 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5821 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5881 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5941 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6001 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6061 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6121 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6181 ccggtgcctg atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     6241 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6301 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6361 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6421 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc
     6481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6661 cggatcttaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     6721 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     6781 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     6841 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     6901 tagggttaat ctagtatacg cgtttaatta atccagcttt tgttcccttt agtgagggtt
     6961 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7021 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7081 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7141 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7801 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9121 ccgaaaagtg ccac
//
