LOCUS       sequence-433210-        4236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC38A9_STOP
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2361..2460)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2477..2496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2478..2496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2501..2517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2501..2517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2619..2638)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2630..3439
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2697..2716)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3586..4174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4075..4094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggcca acatgaatag cgactccagg cacctgggca ccagcgaggt
      721 ggaccacgag agggatccag gacccatgaa catccagttt gagcctagcg atctgcggtc
      781 caagagacca ttctgcatcg agcccaccaa catcgtgaac gtgaatcacg tgatccagcg
      841 ggtgtctgac cacgccagcg ccatgaataa gcggatccac tactattcca gactgaccac
      901 accagcagac aaggccctga tcgcaccaga tcacgtggtg cctgcaccag aggagtgtta
      961 cgtgtattcc cccctgggct ctgcctacaa gctgcagtct tacaccgagg gctatggcaa
     1021 gaacaccagc ctggtgacaa tctttatgat ctggaatacc atgatgggca catccatcct
     1081 gtctatccct tggggcatca agcaggccgg cttcaccaca ggcatgtgcg tgatcatcct
     1141 gatgggcctg ctgaccctgt actgctgtta tagggtggtg aagagccgca caatgatgtt
     1201 ttctctggac accacaagct gggagtaccc cgacgtgtgc aggcactatt ttggctcctt
     1261 cggccagtgg agctccctgc tgttcagcct ggtgtccctg atcggcgcca tgatcgtgta
     1321 ctgggtgctg atgagcaact tcctgtttaa taccggcaag ttcatcttta acttcatcca
     1381 ccacatcaat gacaccgata caatcctgag cacaaacaat tccaaccccg tgatctgccc
     1441 ttctgccgga agcggaggac accctgacaa ctctagcatg atcttttacg ccaatgatac
     1501 cggcgcccag cagtttgaga agtggtggga taagagccgc acagtgccct tctacctggt
     1561 gggcctgctg ctgcccctgc tgaactttaa gtctcctagc ttctttagca agttcaatat
     1621 cctgggcacc gtgtccgtgc tgtacctgat ctttctggtg acattcaagg ccgtgaggct
     1681 gggcttccac ctggagtttc actggttcat ccctaccgag ttctttgtgc cagagatccg
     1741 ctttcagttc ccacagctga ccggcgtgct gacactggcc ttctttatcc acaattgcat
     1801 catcacactg ctgaagaaca ataagaagca ggagaacaat gtgagggacc tgtgcatcgc
     1861 ctatatgctg gtgaccctga catacctgta tatcggcgtg ctggtgtttg cctccttccc
     1921 ctctccccct ctgtccaagg actgtatcga gcagaacttt ctggacaatt tcccttcctc
     1981 tgataccctg tctttcatcg cccggatctt tctgctgttc cagatgatga cagtgtaccc
     2041 actgctgggc tatctggcca gagtgcagct gctgggccac atctttggcg acatctaccc
     2101 ctctatcttc cacgtgctga tcctgaacct gatcatcgtg ggcgccggcg tgatcatggc
     2161 ctgcttctac cccaatatcg gaggaatcat caggtattcc ggagcagcat gtggactggc
     2221 cttcgtgttc atctaccctt ccctgatcta tatcatctct ctgcaccagg aggagagact
     2281 gacctggcca aagctgatct ttcacgtgtt catcatcatc ctgggcgtgg ccaacctgat
     2341 cgtgcagttc tttatgtaag acccagcttt cttgtacaaa gttggcatta taagaaagca
     2401 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2461 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2521 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2581 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2641 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2701 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2761 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2821 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2881 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2941 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3001 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3061 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3121 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3181 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3241 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3301 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3361 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3421 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3481 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3541 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3601 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3661 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3721 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3781 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3841 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3901 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3961 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4021 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4081 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4141 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4201 gttcctggcc ttttgctggc cttttgctca catgtt
//
