LOCUS       sequence-433211-        4710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO6A1_STOP
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2835..2934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2951..2970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2952..2970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2975..2991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2975..2991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3093..3112)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3104..3913
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3171..3190)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4060..4648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4549..4568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgttcg tgggagtggc aaggcacagc ggatcccagg acgaggtgag
      721 caggggagtg gagcctctgg aggcagcaag agcacagcca gcaaaggatc ggagagccaa
      781 gggcacccct aagagctcca agccaggcaa gaagcaccgg tacctgagac tgctgcctga
      841 ggccctgatc aggttcggcg gctttcggaa gagaaagaag gccaagtcta gcgtgtccaa
      901 gaagcccggc gaggtggacg attctctgga gcagccttgt ggcctgggct gcctggtgtc
      961 cacctgctgt gagtgctgta acaatatccg ctgtttcatg atcttttact gtatcctgct
     1021 gatctgccag ggcgtggtgt tcggcctgat cgacgtgtcc atcggcgatt ttcagaagga
     1081 gtaccagctg aagaccatcg agaagctggc cctggagaag tcttatgaca tctcctctgg
     1141 cctggtggcc atcttcatcg ccttttacgg cgatcggaag aaagtgatct ggttcgtggc
     1201 cagctccttt ctgatcggac tgggatctct gctgtgcgca ttcccaagca tcaacgagga
     1261 gaataagcag tccaaagtgg gcatcgagga catctgtgag gagatcaagg tggtgtctgg
     1321 ctgccagtct agcggcatct ctttccagag caagtatctg agcttcttta tcctgggaca
     1381 gaccgtgcag ggaatcgcag gcatgcccct gtacatcctg ggcatcacct ttatcgatga
     1441 gaacgtggcc acacacagcg ccggcatcta tctgggcatc gccgagtgca catccatgat
     1501 cggctacgca ctgggatatg tgctgggagc accactggtg aaggtgcctg agaacaccac
     1561 atccgccacc aataccacag tgaacaatgg ctctcctgag tggctgtgga catggtggat
     1621 caatttcctg tttgccgccg tggtggcctg gtgtaccctg atcccactga gctgcttccc
     1681 aaacaatatg cccggctcca caaggatcaa ggccaggaag cgcaagcagc tgcacttctt
     1741 tgacagccgc ctgaaggatc tgaagctggg caccaacatc aaggacctgt gcgccgccct
     1801 gtggatcctg atgaagaatc ccgtgctgat ctgcctggcc ctgtctaagg ccacagagta
     1861 cctggtcatc atcggcgcca gcgagttcct gcctatctat ctggagaacc agtttatcct
     1921 gaccccaaca gtggcaacca cactggcagg actggtgctg atccctggag gcgccctggg
     1981 acagctgctg ggaggcgtga tcgtgtccac cctggagatg tcttgtaagg ccctgatgcg
     2041 gttcatcatg gtgacatccg tgatctctct gatcctgctg gtgttcatca tctttgtgag
     2101 atgcaaccca gtgcagtttg ccggcatcaa tgaggactac gatggcaccg gcaagctggg
     2161 caacctgaca gccccctgta atgagaagtg ccggtgttcc tctagcatct attcctctat
     2221 ctgcggcaga gacgatatcg agtacttctc tccctgtttt gccggctgca cctatagcaa
     2281 ggcccagaac cagaagaaga tgtactataa ttgcagctgt atcaaggagg gactgatcac
     2341 agcagacgca gagggcgact tcatcgatgc aaggcccggc aagtgtgatg ccaagtgcta
     2401 caagctgcct ctgtttatcg ccttcatctt ttccaccctg atcttcagcg gcttttccgg
     2461 cgtgccaatc gtgctggcca tgacaagggt ggtgccagac aagctgcgca gcctggccct
     2521 gggcgtgtcc tatgtgatcc tgagaatctt cggcaccatc cccggccctt ccatctttaa
     2581 gatgtctggc gagacaagct gcatcctgag ggatgtgaac aagtgtggcc acaccggccg
     2641 ctgctggatc tacaataaga caaagatggc cttcctgctg gtgggcatct gcttcctgtg
     2701 caagctgtgc accatcatct tcaccacaat cgccttcttt atctataaga ggcgcctgaa
     2761 cgagaatacc gactttccag atgtgacagt gaagaacccc aaggtgaaga agaaggagga
     2821 gaccgatctg taagacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2881 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2941 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3001 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     3061 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3121 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3181 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3241 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3301 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3361 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3421 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3481 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3541 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3601 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3661 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3721 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3781 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3841 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3901 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3961 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4201 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4681 ggccttttgc tggccttttg ctcacatgtt
//
