LOCUS       sequence-43322-e       12735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE8
FEATURES             Location/Qualifiers
     source          1..12735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3859
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3850
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3807..3827
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6416..6436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6461..6610
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6632..6685
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6686..6688,6689..7402)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6686..6688,6689..7399)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6728..6749)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6989..7008)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7336..7357
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7427..8015
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7480..7500)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7898..7909)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8018..8034)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8019..8035)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8540..8720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8746..8763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8752..8976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9022..9450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9109..9128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9319..9340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9459..9479)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9464..9793
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9644..9779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9706..9725
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9841..9888
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9907..10281
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10411..10532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10448..10467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10502..10521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10581..10597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10581..10597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10594..10616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10605..10621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10629..10635,10636..10653,10654..10659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10674..10695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10812..10829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10983..11571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11063..11082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11742..12533,12534..12602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12365..12384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12603..12707)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
     3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3841 tccatttcag gtgtcgtgac gtacggccac catgtcgcgg acccggctcc cttccccacc
     3901 cgcacccagc ccagcgtttt cggccgactc gttctcagac ctgcttaggc agttcgaccc
     3961 ctcactgttt aacacatcgt tgttcgactc ccttcctccg tttggggcgc accatacgga
     4021 ggcggccacc ggggagtggg atgaggtgca gtcgggattg agagctgcgg atgcaccacc
     4081 cccaaccatg cgggtggccg tcaccgctgc ccgaccgccg agggcgaagc ccgcaccaag
     4141 gcggagggca gcgcaaccgt ccgacgcaag ccccgcagcg caagtagatt tgagaacttt
     4201 gggatattca cagcagcagc aggaaaagat caagcccaaa gtgaggtcga cagtcgcgca
     4261 gcatcacgaa gcgctggtgg gtcatgggtt tacacatgcc cacatcgtag ccttgtcgca
     4321 gcaccctgca gcccttggca cggtcgccgt caagtaccag gacatgattg cggcgttgcc
     4381 ggaagccaca catgaggcga tcgtcggtgt ggggaaacag tggagcggag cccgagcgct
     4441 tgaggccctg ttgacggtcg cgggagagct gagagggcct ccccttcagc tggacacggg
     4501 ccagttgctg aagatcgcga agcggggagg agtcacggcg gtcgaggcgg tgcacgcgtg
     4561 gcgcaatgcg ctcacgggag cacccctcaa cctgacccca gagcaggtcg tggcaatcgc
     4621 ctccaacatt ggcgggaaac aggcactcga gactgtccag cgcctgcttc ccgtgctgtg
     4681 ccaagcgcac ggactcaccc cagagcaggt cgtggcgatc gcaagccacg acggaggaaa
     4741 gcaagccttg gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc acggcctcac
     4801 cccagagcag gtcgtggcga tcgcaagcca cgacggagga aagcaagcct tggaaacagt
     4861 acagaggctg ttgcctgtgc tgtgccaagc gcacggatta accccagagc aggtcgtggc
     4921 aatcgcctcc aacattggcg ggaaacaggc actcgagact gtccagcgcc tgcttcccgt
     4981 gctgtgccaa gcgcacggct taaccccaga gcaggtcgtg gcgatcgcaa gccacgacgg
     5041 aggaaagcaa gccttggaaa cagtacagag gctgttgcct gtgctgtgcc aagcgcacgg
     5101 acttacccca gagcaggtcg tggccattgc ctcgaatgga gggggcaaac aggcgttgga
     5161 aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac ggccttaccc cagagcaggt
     5221 cgtggcgatc gcaagccacg acggaggaaa gcaagccttg gaaacagtac agaggctgtt
     5281 gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcaa tcgcctccaa
     5341 cattggcggg aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc
     5401 gcacgggctc accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc
     5461 cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacgggc taaccccaga
     5521 gcaggtcgtg gccattgcct cgaatggagg gggcaaacag gcgttggaaa ccgtacaacg
     5581 attgctgccg gtgctgtgcc aagcgcacgg cctaacccca gagcaggtcg tggcaatcgc
     5641 ctccaacatt ggcgggaaac aggcactcga gactgtccag cgcctgcttc ccgtgctgtg
     5701 ccaagcgcac gggttaaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa
     5761 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggactcac
     5821 gcctgagcag gtagtggcta ttgcatccaa tatcgggggc agacccgcac tggagtcaat
     5881 cgtggcccag ctttcgaggc cggaccccgc gctggccgca ctcactaatg atcatcttgt
     5941 agcgctggcc tgcctcggcg gacgacccgc cttggatgcg gtgaagaagg ggctcccgca
     6001 cgcgcctgca ttgattaagc ggaccaacag aaggattccc gagaggacat cacatcgagt
     6061 ggcagatcac gcgcaagtgg tccgcgtgct cggattcttc cagtgtcact cccaccccgc
     6121 acaagcgttc gatgacgcca tgactcaatt tggtatgtcg agacacggac tgctgcagct
     6181 ctttcgtaga gtcggtgtca cagaactcga ggcccgctcg ggcacactgc ctcccgcctc
     6241 ccagcggtgg gacaggattc tccaagcgag cggtatgaaa cgcgcgaagc cttcacctac
     6301 gtcaactcag acacctgacc aggcgagcct tcatgcgttc gcagactcgc tggagaggga
     6361 tttggacgcg ccctcgccca tgcatgaagg ggaccaaact cgcgcgtcag ctagccccaa
     6421 gaagaagaga aaggtggagg ccagcggttc cggacgggct gacgcattgg acgattttga
     6481 tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga
     6541 tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct
     6601 ggacatgctg attaactcta gaggcagtgg agagggcaga ggaagtctgc taacatgcgg
     6661 tgacgtcgag gagaatcctg gcccagtgag caagggcgag gagctgttca ccggggtggt
     6721 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6781 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6841 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6901 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6961 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     7021 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     7081 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     7141 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     7201 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7261 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     7321 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7381 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga
     7441 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7501 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7561 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7621 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7681 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7741 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7801 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7861 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7921 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7981 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     8041 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     8101 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     8161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8221 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     8281 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     8341 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     8401 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8461 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     8521 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8701 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9001 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9421 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9541 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9601 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9721 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9781 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     9841 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     9901 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     9961 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
    10021 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
    10081 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
    10141 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
    10201 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
    10261 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
    10321 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    10381 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    10441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    10561 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    10801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    11461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    11701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    11761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    11821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    11881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    11941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    12001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    12061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    12121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    12181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    12241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    12301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    12361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    12421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    12481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    12541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    12601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    12661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    12721 aaagtgccac ctgac
//
