LOCUS       sequence-433262-       11195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector to secrete human Tspan12 LEL (residues 
            118-218) fused to MBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: MBP-3C-hTspan12LEL(118-218)_pAcGP67a
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     5..832
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5..129
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(167..772)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        836..927
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     852..869
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     1024..1083
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             1093..2190
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     CDS             2212..2235
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_recomb     3038..3743
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3126..3498
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3155..3743)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(4177..4194)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5411..5427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5411..5427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5424..5446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5435..5451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5459..5465,5466..5483,5484..5489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5504..5525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5642..5659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5813..6401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5893..5912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6572..7363,7364..7432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7195..7214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7433..7537)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7605..7623
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7661..7683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7783..7802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7996..8018
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8010..8027
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8011..8027
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
       61 aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
      121 aattattgaa taataaaaca attataaatg tcaaatttgt tttttattaa cgatacaaac
      181 caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
      241 tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
      301 tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
      361 ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
      421 gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
      481 tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
      541 ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
      601 tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
      661 gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
      721 tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
      781 gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcat
      841 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      901 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      961 cgcggatcta tgctactagt aaatcagtca caccaaggct tcaataagga acacacaagc
     1021 aagatggtaa gcgctattgt tttatatgtg cttttggcgg cggcggcgca ttctgccttt
     1081 gcggcggatc ccaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc
     1141 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc
     1201 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc
     1261 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg
     1321 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc
     1381 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt
     1441 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat
     1501 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc
     1561 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac
     1621 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt
     1681 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc
     1741 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac
     1801 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa
     1861 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg
     1921 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac
     1981 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt
     2041 attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg
     2101 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact
     2161 gtcgatgaag ccctgaaaga cgcgcagact aacagcgggt cgacctccgg acttgaagtt
     2221 ctctttcaag gtccgggttc ggaacttatg gttccagtac aatggtcaga tatggtcact
     2281 ttgaaagcca ggatgacaaa ttatggatta cctagatatc ggtggcttac tcatgcttgg
     2341 aatttttttc agagagagtt taagtgctgt ggagtagtat atttcactga ctggttggaa
     2401 atgacagaga tggactggcc cccagattcc tgctgtgtta gagaattccc aggatgttcc
     2461 aaacaggccc accaggaaga tctcagtgac ctttatcaag agggttgtgg gaagaaaatg
     2521 tattcctttt tgagaggaac caaatgagct gcagatctga tcctttcctg ggacccggca
     2581 agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac acgatgaagc
     2641 ttgtcgttgg atggaaagga aaagagttct acagggaaac ttggacccgc ttcatggaag
     2701 acagcttccc cattgttaac gaccaagaag tgatggatgt tttccttgtt gtcaacatgc
     2761 gtcccactag acccaaccgt tgttacaaat tcctggccca acacgctctg cgttgcgacc
     2821 ccgactatgt acctcatgac gtgattagga tcgtcgagcc ttcatgggtg ggcagcaaca
     2881 acgagtaccg catcagcctg gctaagaagg gcggcggctg cccaataatg aaccttcact
     2941 ctgagtacac caactcgttc gaacagttca tcgatcgtgt catctgggag aacttctaca
     3001 agcccatcgt ttacatcggt accgactctg ctgaagagga ggaaattctc cttgaagttt
     3061 ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt
     3121 attaaaacac gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt
     3181 gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt
     3241 ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta
     3301 aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg
     3361 aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt
     3421 gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga
     3481 cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt
     3541 gactcgacgt aaacacgtta aataaagctt ggacatattt aacatcgggc gtgttagctt
     3601 tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg
     3661 attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat
     3721 ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc
     3781 taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta
     3841 acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca
     3901 tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg
     3961 cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg
     4021 tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt
     4081 ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg
     4141 tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg
     4201 ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg
     4261 gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg
     4321 aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat
     4381 acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat
     4441 ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcgcacgcc
     4501 gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc
     4561 gacgtacatg tatgctttgt tgtcaaaaac gtcgttggca agctttaaaa tatttaaaag
     4621 aacatctctg ttcagcacca ctgtgttgtc gtaaatgttg tttttgataa tttgcgcttc
     4681 cgcagtatcg acacgttcaa aaaattgatg cgcatcaatt ttgttgttcc tattattgaa
     4741 taaataagat tgtacagatt catatctacg attcgtcatg gccaccacaa atgctacgct
     4801 gcaaacgctg gtacaatttt acgaaaactg caaaaacgtc aaaactcggt ataaaataat
     4861 caacgggcgc tttggcaaaa tatctatttt atcgcacaag cccactagca aattgtattt
     4921 gcagaaaaca atttcggcgc acaattttaa cgctgacgaa ataaaagttc accagttaat
     4981 gagcgaccac ccaaatttta taaaaatcta ttttaatcac ggttccatca acaaccaagt
     5041 gatcgtgatg gactacattg actgtcccga tttatttgaa acactacaaa ttaaaggcga
     5101 gctttcgtac caacttgtta gcaatattat tagacagctg tgtgaagcgc tcaacgattt
     5161 gcacaagcac aatttcatac acaacgacat aaaactcgaa aatgtcttat atttcgaagc
     5221 acttgatcgc gtgtatgttt gcgattacgg attgtgcaaa cacgaaaact cacttagcgt
     5281 gcacgacggc acgttggagt attttagtcc ggaaaaaatt cgacaccaca actatgcacg
     5341 ttcgtttgac tggtacgccg tcggcgtgtt aacatacaag ttgctaaccg gcggccaatt
     5401 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5461 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5521 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5581 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6241 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7621 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7681 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7741 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     7801 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7861 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     7921 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     7981 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttggct
     8041 cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt gaaagcacgt
     8101 gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt ataaaagatt
     8161 ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac gtgactagcg
     8221 aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt ggagcaataa
     8281 tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg gcgggcctgt
     8341 tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata gttcaagaat
     8401 ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg ggcgtgcact
     8461 gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg cacaccctgg
     8521 gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac aaaattgaaa
     8581 gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc tttaacaaat
     8641 actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta tgcttcgctt
     8701 gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag gattaggccg
     8761 gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt ggttttccac
     8821 gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca cgcacaacac
     8881 cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat ccaccacttt
     8941 ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt tcttttgcat
     9001 ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg tgacctgcaa
     9061 atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa actcttgttt
     9121 tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg tgtgctcggt
     9181 gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt gtttgatcgc
     9241 gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa gccattcttg
     9301 taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc cggatttagt
     9361 aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga cgctgttaga
     9421 ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat tgtctacatg
     9481 aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt ccttggccac
     9541 gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat cttctccaaa
     9601 tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat tttgcaacaa
     9661 ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa tatgggggaa
     9721 catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc ggcgcaagcg
     9781 gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag tacagttttg
     9841 atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt atgacgccta
     9901 caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc cttcctccgt
     9961 gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg cgacgcacaa
    10021 gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt ggcaatattg
    10081 gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg cgttgggcat
    10141 gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat tagtgcgatt
    10201 aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca atcgagtcaa
    10261 gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca gcgcgtattt
    10321 taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc aataatatat
    10381 tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac acgactatga
    10441 tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg atctgtgcac
    10501 gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca tgacccccgt
    10561 agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt atgtcggtga
    10621 cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc tggtgcgaga
    10681 agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt agagcgtcat
    10741 gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat aaattgaccc
    10801 taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc ggactgcgat
    10861 tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc aattttaaaa
    10921 aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa aatgtcgtcg
    10981 acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg aacgatttga
    11041 aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta aactgttaca
    11101 ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag gctctgacgc
    11161 atttctacaa ccacgactcc aagtgtgtgg gtgaa
//
