LOCUS       sequence-433265-       11234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector to secrete MBP-fused human Norrin 
            (residues 118-218) (monomeric mutant C93A/C95A/C131A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: 
            MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
FEATURES             Location/Qualifiers
     source          1..11234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     5..832
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5..129
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(167..772)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        836..927
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     852..869
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     1024..1083
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAVHSAFA"
     CDS             1093..2190
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     CDS             2212..2235
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_recomb     3077..3782
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3165..3537
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3194..3782)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(4216..4233)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5450..5466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5450..5466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5463..5485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5474..5490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5498..5504,5505..5522,5523..5528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5543..5564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5681..5698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5852..6440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5932..5951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6611..7402,7403..7471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7234..7253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7472..7576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7644..7662
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7700..7722)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7822..7841
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8035..8057
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8049..8066
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8050..8066
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
       61 aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
      121 aattattgaa taataaaaca attataaatg tcaaatttgt tttttattaa cgatacaaac
      181 caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
      241 tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
      301 tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
      361 ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
      421 gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
      481 tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
      541 ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
      601 tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
      661 gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
      721 tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
      781 gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcat
      841 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      901 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      961 cgcggatcta tgctactagt aaatcagtca caccaaggct tcaataagga acacacaagc
     1021 aagatggtaa gcgctattgt tttatatgtg cttttggcgg cggcggtgca ttctgccttt
     1081 gcggcggatc ccaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc
     1141 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc
     1201 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc
     1261 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg
     1321 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc
     1381 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt
     1441 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat
     1501 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc
     1561 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac
     1621 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt
     1681 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc
     1741 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac
     1801 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa
     1861 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg
     1921 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac
     1981 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt
     2041 attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg
     2101 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact
     2161 gtcgatgaag ccctgaaaga cgcgcagact aacagcgggt cgacctccgg acttgaagtt
     2221 ctctttcaag gtccgggaat tcaggactcg gaccctcgac gctgcatgag gcaccactat
     2281 gtggattcta tcagtcaccc attgtacaag tgtagctcaa agatggtgct cctggccagg
     2341 tgcgaggggc actgcagcca ggcgtcacgc tccgagcctt tggtgtcgtt cagcactgtc
     2401 ctcaagcaac ccttccgttc ctccgctcac gcctgccggc cccagacttc caagctgaag
     2461 gcactgcggc tgcgatgctc agggggcatg cgactcactg ccacctaccg gtacatcctc
     2521 tcctgtcact gcgaggaagc caattccggt accgagacca gccaggtggc tcctgcatga
     2581 gcggccgctg cagatctgat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct
     2641 tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga tggaaaggaa
     2701 aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg
     2761 accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt
     2821 gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg
     2881 tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg
     2941 ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg
     3001 aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta
     3061 ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg
     3121 agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt
     3181 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
     3241 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
     3301 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
     3361 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
     3421 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
     3481 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
     3541 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
     3601 ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt
     3661 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac
     3721 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa
     3781 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt
     3841 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta
     3901 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg
     3961 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa
     4021 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt
     4081 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt
     4141 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg
     4201 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag
     4261 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga
     4321 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca
     4381 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta
     4441 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag
     4501 caattgtatt gtaaagagat tgtctcaagc tcgcacgccg ataacaagcc ttttcatttt
     4561 tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt
     4621 gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac
     4681 tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa
     4741 aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc
     4801 atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta
     4861 cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat
     4921 atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca
     4981 caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat
     5041 aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga
     5101 ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag
     5161 caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca
     5221 caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg
     5281 cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta
     5341 ttttagtccg gaaaaaattc gacaccacaa ctatgcacgt tcgtttgact ggtacgccgt
     5401 cggcgtgtta acatacaagt tgctaaccgg cggccaattc gtaatcatgg tcatagctgt
     5461 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5521 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5581 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5641 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5701 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5761 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5821 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5881 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5941 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6001 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6061 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6121 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6181 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6241 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6301 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6361 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7621 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7681 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7741 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7801 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7861 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     7921 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     7981 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     8041 cacgacgttg taaaacgacg gccagtgcca agcttggctc gtaaagcgag ttgaaggatc
     8101 atatttagtt gcgtttatga gataagattg aaagcacgtg taaaatgttt cccgcgcgtt
     8161 ggcacaacta tttacaatgc ggccaagtta taaaagattc taatctgata tgttttaaaa
     8221 cacctttgcg gcccgagttg tttgcgtacg tgactagcga agaagatatg tggaccgcag
     8281 aacagatagt aaaacaaaac cctagtattg gagcaataat cgatttaacc aacacgtcta
     8341 aatattatga tggtgtgcat tttttgcggg cgggcctgtt atacaaaaaa attcaagtac
     8401 ctggccagac tttgccgcct gaaagcatag ttcaagaatt tattgacacg gtaaaagaat
     8461 ttacagaaaa gtgtcccggc atgttggtgg gcgtgcactg cacacacggt attaatcgca
     8521 ccggttacat ggtgtgcaga tatttaatgc acaccctggg tattgcgccg caggaagcca
     8581 tagatagatt cgaaaaagcc agaggtcaca aaattgaaag acaaaattac gttcaagatt
     8641 tattaattta attaatatta tttgcattct ttaacaaata ctttatccta ttttcaaatt
     8701 gttgcgcttc ttccagcgaa ccaaaactat gcttcgcttg ctccgtttag cttgtagccg
     8761 atcagtggcg ttgttccaat cgacggtagg attaggccgg atattctcca ccacaatgtt
     8821 ggcaacgttg atgttacgtt tatgcttttg gttttccacg tacgtctttt ggccggtaat
     8881 agccgtaaac gtagtgccgt cgcgcgtcac gcacaacacc ggatgtttgc gcttgtccgc
     8941 ggggtattga accgcgcgat ccgacaaatc caccactttg gcaactaaat cggtgacctg
     9001 cgcgtctttt ttctgcatta tttcgtcttt cttttgcatg gtttcctgga agccggtgta
     9061 catgcggttt agatcagtca tgacgcgcgt gacctgcaaa tctttggcct cgatctgctt
     9121 gtccttgatg gcaacgatgc gttcaataaa ctcttgtttt ttaacaagtt cctcggtttt
     9181 ttgcgccacc accgcttgca gcgcgtttgt gtgctcggtg aatgtcgcaa tcagcttagt
     9241 caccaactgt ttgctctcct cctcccgttg tttgatcgcg ggatcgtact tgccggtgca
     9301 gagcacttga ggaattactt cttctaaaag ccattcttgt aattctatgg cgtaaggcaa
     9361 tttggacttc ataatcagct gaatcacgcc ggatttagta atgagcactg tatgcggctg
     9421 caaatacagc gggtcgcccc ttttcacgac gctgttagag gtagggcccc cattttggat
     9481 ggtctgctca aataacgatt tgtatttatt gtctacatga acacgtatag ctttatcaca
     9541 aactgtatat tttaaactgt tagcgacgtc cttggccacg aaccggacct gttggtcgcg
     9601 ctctagcacg taccgcaggt tgaacgtatc ttctccaaat ttaaattctc caattttaac
     9661 gcgagccatt ttgatacacg tgtgtcgatt ttgcaacaac tattgttttt taacgcaaac
     9721 taaacttatt gtggtaagca ataattaaat atgggggaac atgcgccgct acaacactcg
     9781 tcgttatgaa cgcagacggc gccggtctcg gcgcaagcgg ctaaaacgtg ttgcgcgttc
     9841 aacgcggcaa acatcgcaaa agccaatagt acagttttga tttgcatatt aacggcgatt
     9901 ttttaaatta tcttatttaa taaatagtta tgacgcctac aactccccgc ccgcgttgac
     9961 tcgctgcacc tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg
    10021 gtggtcgatg accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc
    10081 gtcgggcgaa ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca
    10141 gttgggttgt ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg
    10201 catgcaagcc gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct
    10261 tttaatcatg ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat
    10321 gttttctttg tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta
    10381 agttagtttc atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat
    10441 taacaatgcg cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga
    10501 attaaatagc ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga
    10561 ttgtaataag tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa
    10621 aagaactgcc gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc
    10681 caatcgaccg ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg
    10741 catcgtataa cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat
    10801 atttaattga tcccgatgat tttattgata aattgaccct aactccatac acggtattct
    10861 acaatggcgg ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc
    10921 cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt
    10981 gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta
    11041 atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg
    11101 gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg
    11161 ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca
    11221 agtgtgtggg tgaa
//
