LOCUS       sequence-433266-       11838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 
            Delta-C (residues 40-513) fused to split GFP (strands 1-10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: FLAG-mFzd4-Delta-C(40-513)-3C-sGFP1-10_pVL1393
FEATURES             Location/Qualifiers
     source          1..11838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5651..5674
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(5714..5742)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     misc_recomb     6846..7551
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           6934..7306
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(6963..7551)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(7985..8002)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9219..9235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9219..9235)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9232..9254)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9243..9259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9267..9273,9274..9291,9292..9297))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9312..9333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9450..9467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9621..10209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9701..9720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10380..11171,11172..11240))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11003..11022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11241..11345)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11413..11431
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11469..11491)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11591..11610
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11804..11826
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11818..11835
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11819..11835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccgggta
     4141 ccttgccacc atgaagacga tcatcgccct gagctacatc ttctgcctgg tgttcgccga
     4201 ctacaaggac gatgatgacg ccatggagga gcggcgctgc gaccccatcc gcatcgccat
     4261 gtgccagaac ctcggctaca acgtgaccaa gatgcccaac ttagtgggac acgagctgca
     4321 gacagacgcc gagctgcagc tgacaacttt cacgccgctc atccagtacg gctgctccag
     4381 ccagctgcag ttcttccttt gttcggttta tgtgccaatg tgcacagaga agatcaacat
     4441 ccccatcggc ccgtgcggtg gcatgtgcct ttcagtcaag agacgctgtg aaccagtcct
     4501 gagagaattt gggtttgcct ggcccgacac cctgaactgc agcaagttcc cgccccagaa
     4561 cgaccacaac cacatgtgca tggaaggacc aggtgatgaa gaggttccct tgccccacaa
     4621 gactcccatc cagcccgggg aagagtgcca ctccgtggga agcaattctg atcagtacat
     4681 ctgggtgaag aggagcctga actgtgttct caagtgtggc tacgatgctg gcttgtacag
     4741 ccgctcagct aaggagttca cggatatttg gatggctgtg tgggccagcc tctgcttcat
     4801 ctccaccacc ttcaccgtgc tgaccttcct gattgattca tccaggtttt cttaccctga
     4861 gcgccccatc atatttctca gtatgtgcta taatatttat agcattgctt atattgttcg
     4921 gctgactgta ggccgggaaa ggatatcctg tgattttgaa gaggcggcag agcccgttct
     4981 catccaagaa ggacttaaga acacaggatg tgcaataatt ttcttgctga tgtacttttt
     5041 tggaatggcc agctccattt ggtgggttat tctgacactc acttggtttt tggcagccgg
     5101 actcaagtgg ggtcatgaag ccattgaaat gcacagttct tatttccaca tcgcagcctg
     5161 ggctattccc gcagtgaaaa ccattgtcat cttgattatg agactagtgg atgccgatga
     5221 actgactggc ttgtgctatg ttgggaacca aaacctagat gccctcactg gctttgtggt
     5281 ggctcctctc tttacgtatt tggtgattgg aacgctgttc attgcggcgg gtttggtggc
     5341 cttattcaaa attcggtcca atcttcaaaa agacgggaca aagacagaca agttggaaag
     5401 gctaatggtc aagatcgggg tcttctcagt actgtacacg gttcctgcaa cctgtgtgat
     5461 tgcctgttat ttctatgaaa tctcaaactg ggcactcttt cgatattctg cagatgactc
     5521 aaacatggca gttgaaatgt tgaaaatttt tatgtctttg ctcgtgggca tcacttcagg
     5581 catgtggatt tggtctgcca aaactcttca cacgtggcaa aagtgttcta accgattggt
     5641 gaatggatcc ctcgaagtcc ttttccaggg ccctggtggc actagtagca aaggagaaga
     5701 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     5761 attttctgtc agaggagagg gtgaaggtga tgctacaatc ggaaaactca cccttaaatt
     5821 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctctgaccta
     5881 tggtgttcaa tgcttttccc gttatccgga tcacatgaaa aggcatgact ttttcaagag
     5941 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaaata
     6001 caagacgcgt gctgtagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa
     6061 gggtactgat tttaaagaag atggaaacat tctcggacac aaactcgagt acaactttaa
     6121 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcac
     6181 agttcgccac aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac
     6241 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaaactgt
     6301 cctttcgaaa gatcccaacg aaaagggctc cggctccggc gagaccgtgg tgtgagctgc
     6361 agatctgatc ctttcctggg acccggcaag aaccaaaaac tcactctctt caaggaaatc
     6421 cgtaatgtta aacccgacac gatgaagctt gtcgttggat ggaaaggaaa agagttctac
     6481 agggaaactt ggacccgctt catggaagac agcttcccca ttgttaacga ccaagaagtg
     6541 atggatgttt tccttgttgt caacatgcgt cccactagac ccaaccgttg ttacaaattc
     6601 ctggcccaac acgctctgcg ttgcgacccc gactatgtac ctcatgacgt gattaggatc
     6661 gtcgagcctt catgggtggg cagcaacaac gagtaccgca tcagcctggc taagaagggc
     6721 ggcggctgcc caataatgaa ccttcactct gagtacacca actcgttcga acagttcatc
     6781 gatcgtgtca tctgggagaa cttctacaag cccatcgttt acatcggtac cgactctgct
     6841 gaagaggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga gtttgcacca
     6901 gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta ttagtacatt
     6961 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
     7021 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
     7081 gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga
     7141 ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg
     7201 ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt
     7261 tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt
     7321 ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taaagcttgg
     7381 acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc
     7441 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
     7501 gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat
     7561 tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg
     7621 ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc
     7681 ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga
     7741 ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta
     7801 ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc
     7861 ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg
     7921 tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac
     7981 atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc
     8041 ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc
     8101 ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt
     8161 ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta
     8221 atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg
     8281 taaagagatt gtctcaagct cgcacgccga taacaagcct tttcattttt actacagcat
     8341 tgtagtggcg agacacttcg ctgtcgtcga cgtacatgta tgctttgttg tcaaaaacgt
     8401 cgttggcaag ctttaaaata tttaaaagaa catctctgtt cagcaccact gtgttgtcgt
     8461 aaatgttgtt tttgataatt tgcgcttccg cagtatcgac acgttcaaaa aattgatgcg
     8521 catcaatttt gttgttccta ttattgaata aataagattg tacagattca tatctacgat
     8581 tcgtcatggc caccacaaat gctacgctgc aaacgctggt acaattttac gaaaactgca
     8641 aaaacgtcaa aactcggtat aaaataatca acgggcgctt tggcaaaata tctattttat
     8701 cgcacaagcc cactagcaaa ttgtatttgc agaaaacaat ttcggcgcac aattttaacg
     8761 ctgacgaaat aaaagttcac cagttaatga gcgaccaccc aaattttata aaaatctatt
     8821 ttaatcacgg ttccatcaac aaccaagtga tcgtgatgga ctacattgac tgtcccgatt
     8881 tatttgaaac actacaaatt aaaggcgagc tttcgtacca acttgttagc aatattatta
     8941 gacagctgtg tgaagcgctc aacgatttgc acaagcacaa tttcatacac aacgacataa
     9001 aactcgaaaa tgtcttatat ttcgaagcac ttgatcgcgt gtatgtttgc gattacggat
     9061 tgtgcaaaca cgaaaactca cttagcgtgc acgacggcac gttggagtat tttagtccgg
     9121 aaaaaattcg acaccacaac tatgcacgtt cgtttgactg gtacgccgtc ggcgtgttaa
     9181 catacaagtt gctaaccggc ggccaattcg taatcatggt catagctgtt tcctgtgtga
     9241 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9301 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9361 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9421 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9481 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10501 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11341 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    11401 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    11461 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    11521 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    11581 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    11641 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    11701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    11761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    11821 aaaacgacgg ccagtgcc
//
