LOCUS       sequence-433268-       10503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector to express human Tspan12 ∆C (residues 
            1-252) fused to split GFP s11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
FEATURES             Location/Qualifiers
     source          1..10503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             910..927
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             934..957
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             970..1017
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_recomb     1511..2216
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           1599..1971
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(1628..2216)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(2650..2667)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3884..3900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3884..3900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3897..3919)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3908..3924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3932..3938,3939..3956,3957..3962))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3977..3998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4115..4132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4286..4874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4366..4385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5045..5836,5837..5905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5668..5687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5906..6010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6078..6096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6134..6156)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6256..6275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6469..6491
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6483..6500
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6484..6500
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     9673..10500
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             9673..9797
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9835..10440)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcccgggta ccttgccacc atggccagag aagattccgt gaagtgtctg
      181 cgctgcctgc tctacgccct caatctgctc ttttggttaa tgtccatcag tgtgttggca
      241 gtttctgctt ggatgaggga ctacctaaat aatgttctca ctttaactgc agaaacgagg
      301 gtagaggaag cagtcatttc gacttacttt cctgtggttc atccggtcat gattgctgtt
      361 tgctgtttcc ttatcattgt ggggatgtta ggatattgtg gaacggtgaa aagaaatctg
      421 ttgcttcttg catggtactt tggaagtttg cttgtcattt tctgtgtaga actggcttgt
      481 ggcgtttgga catatgaaca ggaacttatg gttccagtac aatggtcaga tatggtcact
      541 ttgaaagcca ggatgacaaa ttatggatta cctagatatc ggtggcttac tcatgcttgg
      601 aatttttttc agagagagtt taagtgctgt ggagtagtat atttcactga ctggttggaa
      661 atgacagaga tggactggcc cccagattcc tgctgtgtta gagaattccc aggatgttcc
      721 aaacaggccc accaggaaga tctcagtgac ctttatcaag agggttgtgg gaagaaaatg
      781 tattcctttt tgagaggaac caaacaactg caggtgctga ggtttctggg aatctccatt
      841 ggggtgacac aaatcctggc catgattctc accattactc tgctctgggc tctgtattat
      901 gatagaggtc accaccatca tcaccatggt tccctcgaag tccttttcca gggccctggt
      961 tctggttctc gtgaccacat ggtgctgcat gaatatgtga acgcggcggg catcacgtga
     1021 gctgcagatc tgatcctttc ctgggacccg gcaagaacca aaaactcact ctcttcaagg
     1081 aaatccgtaa tgttaaaccc gacacgatga agcttgtcgt tggatggaaa ggaaaagagt
     1141 tctacaggga aacttggacc cgcttcatgg aagacagctt ccccattgtt aacgaccaag
     1201 aagtgatgga tgttttcctt gttgtcaaca tgcgtcccac tagacccaac cgttgttaca
     1261 aattcctggc ccaacacgct ctgcgttgcg accccgacta tgtacctcat gacgtgatta
     1321 ggatcgtcga gccttcatgg gtgggcagca acaacgagta ccgcatcagc ctggctaaga
     1381 agggcggcgg ctgcccaata atgaaccttc actctgagta caccaactcg ttcgaacagt
     1441 tcatcgatcg tgtcatctgg gagaacttct acaagcccat cgtttacatc ggtaccgact
     1501 ctgctgaaga ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg
     1561 caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt
     1621 acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt
     1681 ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat
     1741 tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt
     1801 ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat
     1861 tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat
     1921 tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg
     1981 tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaataaag
     2041 cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc
     2101 aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat
     2161 taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattattt
     2221 ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt aattcagaca
     2281 acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa tctactaatg
     2341 gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc ggggctggcg
     2401 gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct
     2461 ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc gtttttggtt
     2521 tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac aattgttgtc
     2581 tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt
     2641 cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg ggagaaggtg
     2701 gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg
     2761 gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt
     2821 ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct gctataagca
     2881 ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg taagcaattg
     2941 tattgtaaag agattgtctc aagctcgcac gccgataaca agccttttca tttttactac
     3001 agcattgtag tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa
     3061 aacgtcgttg gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt
     3121 gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg
     3181 atgcgcatca attttgttgt tcctattatt gaataaataa gattgtacag attcatatct
     3241 acgattcgtc atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa
     3301 ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat
     3361 tttatcgcac aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt
     3421 taacgctgac gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat
     3481 ctattttaat cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc
     3541 cgatttattt gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat
     3601 tattagacag ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga
     3661 cataaaactc gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta
     3721 cggattgtgc aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag
     3781 tccggaaaaa attcgacacc acaactatgc acgttcgttt gactggtacg ccgtcggcgt
     3841 gttaacatac aagttgctaa ccggcggcca attcgtaatc atggtcatag ctgtttcctg
     3901 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3961 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4021 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4081 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4141 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4201 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4261 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4741 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4801 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4861 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4921 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4981 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5041 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5101 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5161 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5221 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5281 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5341 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5401 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5461 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5521 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5581 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5641 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5701 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5761 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5821 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5881 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5941 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6001 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6061 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     6121 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6181 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6241 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6301 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     6361 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6421 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6481 gttgtaaaac gacggccagt gccaagcttg gctcgtaaag cgagttgaag gatcatattt
     6541 agttgcgttt atgagataag attgaaagca cgtgtaaaat gtttcccgcg cgttggcaca
     6601 actatttaca atgcggccaa gttataaaag attctaatct gatatgtttt aaaacacctt
     6661 tgcggcccga gttgtttgcg tacgtgacta gcgaagaaga tatgtggacc gcagaacaga
     6721 tagtaaaaca aaaccctagt attggagcaa taatcgattt aaccaacacg tctaaatatt
     6781 atgatggtgt gcattttttg cgggcgggcc tgttatacaa aaaaattcaa gtacctggcc
     6841 agactttgcc gcctgaaagc atagttcaag aatttattga cacggtaaaa gaatttacag
     6901 aaaagtgtcc cggcatgttg gtgggcgtgc actgcacaca cggtattaat cgcaccggtt
     6961 acatggtgtg cagatattta atgcacaccc tgggtattgc gccgcaggaa gccatagata
     7021 gattcgaaaa agccagaggt cacaaaattg aaagacaaaa ttacgttcaa gatttattaa
     7081 tttaattaat attatttgca ttctttaaca aatactttat cctattttca aattgttgcg
     7141 cttcttccag cgaaccaaaa ctatgcttcg cttgctccgt ttagcttgta gccgatcagt
     7201 ggcgttgttc caatcgacgg taggattagg ccggatattc tccaccacaa tgttggcaac
     7261 gttgatgtta cgtttatgct tttggttttc cacgtacgtc ttttggccgg taatagccgt
     7321 aaacgtagtg ccgtcgcgcg tcacgcacaa caccggatgt ttgcgcttgt ccgcggggta
     7381 ttgaaccgcg cgatccgaca aatccaccac tttggcaact aaatcggtga cctgcgcgtc
     7441 ttttttctgc attatttcgt ctttcttttg catggtttcc tggaagccgg tgtacatgcg
     7501 gtttagatca gtcatgacgc gcgtgacctg caaatctttg gcctcgatct gcttgtcctt
     7561 gatggcaacg atgcgttcaa taaactcttg ttttttaaca agttcctcgg ttttttgcgc
     7621 caccaccgct tgcagcgcgt ttgtgtgctc ggtgaatgtc gcaatcagct tagtcaccaa
     7681 ctgtttgctc tcctcctccc gttgtttgat cgcgggatcg tacttgccgg tgcagagcac
     7741 ttgaggaatt acttcttcta aaagccattc ttgtaattct atggcgtaag gcaatttgga
     7801 cttcataatc agctgaatca cgccggattt agtaatgagc actgtatgcg gctgcaaata
     7861 cagcgggtcg ccccttttca cgacgctgtt agaggtaggg cccccatttt ggatggtctg
     7921 ctcaaataac gatttgtatt tattgtctac atgaacacgt atagctttat cacaaactgt
     7981 atattttaaa ctgttagcga cgtccttggc cacgaaccgg acctgttggt cgcgctctag
     8041 cacgtaccgc aggttgaacg tatcttctcc aaatttaaat tctccaattt taacgcgagc
     8101 cattttgata cacgtgtgtc gattttgcaa caactattgt tttttaacgc aaactaaact
     8161 tattgtggta agcaataatt aaatatgggg gaacatgcgc cgctacaaca ctcgtcgtta
     8221 tgaacgcaga cggcgccggt ctcggcgcaa gcggctaaaa cgtgttgcgc gttcaacgcg
     8281 gcaaacatcg caaaagccaa tagtacagtt ttgatttgca tattaacggc gattttttaa
     8341 attatcttat ttaataaata gttatgacgc ctacaactcc ccgcccgcgt tgactcgctg
     8401 cacctcgagc agttcgttga cgccttcctc cgtgtggccg aacacgtcga gcgggtggtc
     8461 gatgaccagc ggcgtgccgc acgcgacgca caagtatctg tacaccgaat gatcgtcggg
     8521 cgaaggcacg tcggcctcca agtggcaata ttggcaaatt cgaaaatata tacagttggg
     8581 ttgtttgcgc atatctatcg tggcgttggg catgtacgtc cgaacgttga tttgcatgca
     8641 agccgaaatt aaatcattgc gattagtgcg attaaaacgt tgtacatcct cgcttttaat
     8701 catgccgtcg attaaatcgc gcaatcgagt caagtgatca aagtgtggaa taatgttttc
     8761 tttgtattcc cgagtcaagc gcagcgcgta ttttaacaaa ctagccatct tgtaagttag
     8821 tttcatttaa tgcaacttta tccaataata tattatgtat cgcacgtcaa gaattaacaa
     8881 tgcgcccgtt gtcgcatctc aacacgacta tgatagagat caaataaagc gcgaattaaa
     8941 tagcttgcga cgcaacgtgc acgatctgtg cacgcgttcc ggcacgagct ttgattgtaa
     9001 taagttttta cgaagcgatg acatgacccc cgtagtgaca acgatcacgc ccaaaagaac
     9061 tgccgactac aaaattaccg agtatgtcgg tgacgttaaa actattaagc catccaatcg
     9121 accgttagtc gaatcaggac cgctggtgcg agaagccgcg aagtatggcg aatgcatcgt
     9181 ataacgtgtg gagtccgctc attagagcgt catgtttaga caagaaagct acatatttaa
     9241 ttgatcccga tgattttatt gataaattga ccctaactcc atacacggta ttctacaatg
     9301 gcggggtttt ggtcaaaatt tccggactgc gattgtacat gctgttaacg gctccgccca
     9361 ctattaatga aattaaaaat tccaatttta aaaaacgcag caagagaaac atttgtatga
     9421 aagaatgcgt agaaggaaag aaaaatgtcg tcgacatgct gaacaacaag attaatatgc
     9481 ctccgtgtat aaaaaaaata ttgaacgatt tgaaagaaaa caatgtaccg cgcggcggta
     9541 tgtacaggaa gaggtttata ctaaactgtt acattgcaaa cgtggtttcg tgtgccaagt
     9601 gtgaaaaccg atgtttaatc aaggctctga cgcatttcta caaccacgac tccaagtgtg
     9661 tgggtgaagt catgcatctt ttaatcaaat cccaagatgt gtataaacca ccaaactgcc
     9721 aaaaaatgaa aactgtcgac aagctctgtc cgtttgctgg caactgcaag ggtctcaatc
     9781 ctatttgtaa ttattgaata ataaaacaat tataaatgtc aaatttgttt tttattaacg
     9841 atacaaacca aacgcaacaa gaacatttgt agtattatct ataattgaaa acgcgtagtt
     9901 ataatcgctg aggtaatatt taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa
     9961 tataatttta ttttcacata aactagacgc cttgtcgtct tcttcttcgt attccttctc
    10021 tttttcattt ttctcctcat aaaaattaac atagttatta tcgtatccat atatgtatct
    10081 atcgtataga gtaaattttt tgttgtcata aatatatatg tcttttttaa tggggtgtat
    10141 agtaccgctg cgcatagttt ttctgtaatt tacaacagtg ctattttctg gtagttcttc
    10201 ggagtgtgtt gctttaatta ttaaatttat ataatcaatg aatttgggat cgtcggtttt
    10261 gtacaatatg ttgccggcat agtacgcagc ttcttctagt tcaattacac cattttttag
    10321 cagcaccgga ttaacataac tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc
    10381 acctcccttt tctatactat tgtctgcgag cagttgtttg ttgttaaaaa taacagccat
    10441 tgtaatgaga cgcacaaact aatatcacaa actggaaatg tctatcaata tatagttgct
    10501 gat
//
