LOCUS       sequence-433270-       13575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with 
            an sgRNA cloning cassette, facilitating high-efficiency and 
            high-fidelity base editing at targets bearing NG PAM in 
            Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13575)
  AUTHORS   Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
  TITLE     Engineered cytosine base editor enabling broad-scope and 
            high-fidelity gene editing in Streptomyces.
  JOURNAL   Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
  PUBMED    38971862
REFERENCE   2  (bases 1 to 13575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-7"; volume: "15(1):5687. doi"; pages: " 
            10.1038/s41467-024-49987-3"
COMMENT     Sequence Label: peSCBE3-NG-HF1-Hypa
FEATURES             Location/Qualifiers
     source          1..13575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..804)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     primer_bind     complement(998..1018)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(998..1016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1045..1063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1236..1304,1305..2096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1454..1473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2756..2775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3009..3026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3240..3374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3264..3283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3318..3337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6592..6893
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     CDS             11652..11903
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     oriT            12266..12375
                     /note="incP origin of transfer"
     CDS             12408..12779
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_RNA        12935..13010
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag
       61 gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg
      121 gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag
      181 ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg
      241 taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc
      301 catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac
      361 ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg
      421 caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg
      481 ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg
      541 tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat
      601 gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc
      661 atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt
      721 aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac
      781 attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa
      841 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
      901 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
      961 acggcatcag ttaccgtgag ctgcaggtcg acggatccct ttagtgaggg ttaattgcgg
     1021 ccgcgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ctgacttccg cgtttccaga
     3061 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     3121 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     3181 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtcag
     3241 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3301 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3361 gcaataaaca agttcccgca cgatcagcgt cccgccgacc aggagcagtg cggccagcac
     3421 tgccgctaac gcctggtcct ggtcccggtc ctggtggtgc atcagtcctc cccgtgatca
     3481 cttcggcacc caccgtagtg atcacccccg acagcggatc aaggggtttg cgggtcccgg
     3541 tcggcgccgg gcgggggagg caggagccgc cgacgctgcc tctgggacgg gccggacggc
     3601 agggggaccg gcggccgggc gagctgcagc cgggggtccg gcagggccgg agcgggcgga
     3661 accgtgctct gacctgcggc ccgagtttcg tcacgtgacg gaatggaagg ctgctgcatt
     3721 tcgtcacgtg acgtatctcg gcgagcgact gccgacgcca cggcggacac gatcgcctcg
     3781 cgctggcgcc gggcctcgta cgcccgctgg cggcaggagc ggcggcagta gtcccggctc
     3841 cggccgacgc cggattgctt gatctccgag ccgcaccagg cgcagagctt cgcgccgtcg
     3901 gcgtccctgg gggtggtggt gctcatggcc gacgaccgta cgcggcacgt ctcgtagcga
     3961 ggcgagtcgg gcgcgaggta ccgcctgcac gaagtgccgg cggggccgac cccgggcgag
     4021 taatcccagg attactcccg cggcttcgac cccggccgcc gtcgccgcgt acgtcaccga
     4081 cccccgccgt acgtcaccgg gatgacgtac ggcggggggg agcgagttag tgcgaagtgg
     4141 gcccacttgc gagccgggcg atgtgccggg cggcccgctc ctggcggtcg tcggcgtcgt
     4201 cgtcctggtc gtcgtcctgc tctcgccgtc ggcgtgcagt tgcttcctcg cggcgctggg
     4261 cgagggcggc gagcatgtcg gcgtacgcct cggccacctc ccccgccgtg agcaccacca
     4321 ctgtgtcggc cgcgtcggcc agcgccagga cctcccgcac ccgttcgccc acggccgccg
     4381 aatcctcgtt gccgtccttg ccttcggcgg cccgggtcgc ctcgaggtcg agggcgcggc
     4441 gggtgaccgc gtgccatccg tcctcggtca cggcgacccc ggcccgcagc tccccgccgt
     4501 cggcgtcggc cgccaggagc agatcgaggt cgtcggcctc ggtgtcgccg ccgtcgagcc
     4561 cgagcatctg ccgcaggtag cgggtccatt cgatggcccg gcgtccccgg gttgcccgct
     4621 cgtactcgtg ccagcgcgag aggttccact ccagcgagcc gaccccggcg gcgtcgtcct
     4681 cggtcatgcc gccggtcagg tccccgatcc gtccgaggag ttcgaacggg gcgacgttcc
     4741 cgccggtcgc cgtcttgagg tcggcgcggg cgagttcgag ggcgggcgcc ttcccgtcct
     4801 gggtcttggc gatgtactcg gcgaggtcgt tggcgtcgcg ctcggtctcc agccgcttga
     4861 agtcgacgcc gtgccggtcg tcgggcgtga aggcggggtt gaccttgcgc agggcggcgg
     4921 tccacacgga ccgccagtgc ccctgccact cgtcgagcgc ggcgccggtc ggctcgaagg
     4981 tggcgacgat ctgcttcgcg gaccgctccc cctcggtccg gccgccgacc aggacgatcg
     5041 cgtggatgtg cgggtgccag ccgttgatct gccccacggt gacttcggtc gcgcggatca
     5101 tgccgacgta cccgatccgg tctcggatgc cctcgcggtc ggcggcccgg tgcccgtcct
     5161 tggcccggcg tccggcccac gtgccgcccg tgatcagtcg ctggtaggcg cccggccgcc
     5221 gggggctgtc cggcgtcttc cgggtgccct ggagggcgtc catgaggtcc gcgagccggt
     5281 ccgtgtgccc atggcgggcc gtgaaggtga ccaggtaggc ggtccccccg cgcttgatcc
     5341 actcgaccac ggcggcggtg atctcctcgg cccgcttgtg ccggatcgtg gcggcgcaga
     5401 ccgggcagag ccagatccgc ccgcaccgca tcaggcccag gaccacggac gttccggccg
     5461 ccgtctgggc gacgatcacg ccggaggcag ggtccatcag ggcgcggccg cagcccttgc
     5521 acgcggcgtc cccgctgatc cgccacagcg tccggcggcg gctgtaccgg gcggctttcc
     5581 gcagtcgggc agcgtcgctc cgcgacgtgc ttcctacttc cgagaggctg tcgcctctcg
     5641 ggctctcccc atccaccccg tccggagaaa ccgcaggtcg gaggggtgcg ggaaactctg
     5701 ttgtttcttt cccaaggtgt tcgcttttgc ctcgggcggc atctcgcgtc acacgcgcga
     5761 tcgcccgctt cgctgccatc cggcagcggt ctgagcagta gatacgcggc cgtttgcccg
     5821 gtgtgtgggc aattgcggtc ccgcagtggc agcggggccc ggcgggccga tctggcaatg
     5881 cctcggcatc gctccgtact ctgggcacga gcaacgttcc tgtctcgccc ggctaagggg
     5941 cgcgagtctg ggagcggacg ggtcggaggt gcgaagtccg gcccgttgct ctttggtctg
     6001 gtgggaatcc tggcaccaat cgggccagag gttccctccg ccactcccga cgccccttgg
     6061 ggctggtgtg acttggaggg ccgaagagag ccccgccggt gatccggcgg ggctttgacg
     6121 tgcggtcagt gcgtgtgtcg gcgagcgatg gccacgaggc cctggaagcc gagcggtccg
     6181 gcgaagtcgg cccagtcgca accgggctca gcgcagtggg cggaccagcc accgccgttg
     6241 gggtcctgga ccaggttcac ggtcccctcg gtcaggcgtc cgtcgaagtc ggtcatggtc
     6301 ggtctcctgg tgggtggggg cggggcgcca gcacgaagtg ccggcgcccc gcgggggttg
     6361 gtcgggtcag gcgccgaacc ggcgggcggc ggcggcgacc aggccgtcgg cggcggccat
     6421 ggcgcggtcg cggtcggtgg tgagggcggt gcggtcggcg gcggcccagt cctgctctca
     6481 cgcaacgtct acgtggacgc tcagggcgac acgatcgagg tcggggagtc cgtcgctccg
     6541 gcgggccgct ggcgcgtcca ccgggactga tcaaggcgaa tacttcatat ggccctgcag
     6601 gcggaagtca ggtagacacg acttccgcta gtccttgcaa ggtctgctga cgtgaggcgg
     6661 ggcggtcgtt tttgaccgcc ctgccttcgt catgtaggct cgctcgctgt gcctggcgtg
     6721 tcatcagacg cccaggtccc ggtgccgtga ggcccgggcc atcgagccgg tggtacgtgg
     6781 ctgcggtccc cttgtgaggg ctgcgcgccg tgtgctgtcc ggcgcgcaca gccttgaatc
     6841 cacccgcggg ggccggccgg tctccgtgag ctcgagtaga cgacggagac gtaatggagg
     6901 cctcccccgc ctccggcccc cgccacctga tggacccgca catcttcacc tccaacttca
     6961 acaacggcat cggccgccac aagacctacc tgtgctacga ggtcgagcgg ctggacaacg
     7021 gcacctccgt caagatggac cagcaccggg gcttcctgca caaccaggcc aagaacctgc
     7081 tgtgcggctt ctacggccgc cacgccgagc tgcggttcct ggacctggtc ccctccctgc
     7141 agctggaccc cgcccagatc taccgcgtca cctggttcat ctcctggtcc ccgtgcttct
     7201 cctggggctg cgccggcgag gtccgggcct tcctgcagga gaacacccac gtccgcctgc
     7261 ggatcttcgc cgcccgcatc ttcgactacg accccctgta caaggaagcc ctgcagatgc
     7321 tgcgggacgc cggcgcccag gtctccatca tgacctacga cgagttcaag cactgctggg
     7381 acaccttcgt ggaccaccag ggctgcccct tccagccctg ggacggcctg gacgagcact
     7441 cccaggccct gtccggccgc ctgcgggcca tcctgcagaa ccagggcaac tccggctcgg
     7501 agaccccggg cacctcggag tccgccaccc cggagtccga caagaagtac agcatcggcc
     7561 tggccatcgg caccaacagc gtgggctggg cggtcatcac cgacgagtac aaggtcccct
     7621 ccaagaagtt caaggtcctg ggcaacaccg accggcactc gatcaagaag aacctgatcg
     7681 gcgccctgct cttcgacagc ggcgagaccg ccgaggcgac ccgcctgaag cggaccgcgc
     7741 gccggcgcta cacccggcgc aagaaccgca tctgctacct ccaggaaatc ttctccaacg
     7801 agatggccaa ggtcgacgac tcgttcttcc accggctcga ggagagcttc ctggtggagg
     7861 aggacaagaa gcacgagcgc cacccgatct tcggcaacat cgtcgacgag gtggcctacc
     7921 acgagaagta ccccaccatc taccacctcc gcaagaagct ggtcgactcg accgacaagg
     7981 cggacctgcg gctcatctac ctggccctcg cgcacatgat caagttccgc ggccacttcc
     8041 tcatcgaggg cgacctgaac ccggacaact ccgacgtcga caagctcttc atccagctgg
     8101 tgcagaccta caaccagctg ttcgaggaga accccatcaa cgccagcggc gtcgacgcca
     8161 aggcgatcct ctccgcgcgc ctgagcaagt cccggcgcct ggagaacctc atcgcccagc
     8221 tgccgggcga gaagaagaac ggcctcttcg gcaacctgat cgcgctgtcg ctcggcctga
     8281 cccccaactt caagagcaac ttcgacctgg ccgaggacgc gaagctccag ctgtccaagg
     8341 acacctacga cgacgacctg gacaacctgc tcgcccagat cggcgaccag tacgcggacc
     8401 tcttcctggc cgcgaagaac ctctcggacg ccatcctgct cagcgacatc ctgcgggtca
     8461 acaccgagat caccaaggcc ccgctgtcgg cgagcatgat caagcggtac gacgagcacc
     8521 accaggacct gaccctgctc aaggccctcg tgcgccagca gctgcccgag aagtacaagg
     8581 aaatcttctt cgaccagtcc aagaacggct acgccggcta catcgacggc ggcgcgtcgc
     8641 aggaggagtt ctacaagttc atcaagccga tcctggagaa gatggacggc accgaggagc
     8701 tgctcgtcaa gctgaaccgc gaggacctgc tccgcaagca gcggaccttc gacaacggct
     8761 ccatcccgca ccagatccac ctgggcgagc tgcacgccat cctccggcgc caggaggact
     8821 tctacccctt cctgaaggac aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc
     8881 cgtactacgt cggccccctg gcccgcggca actcccggtt cgcgtggatg acccggaagt
     8941 cggaggagac catcaccccg tggaacttcg aggaggtcgt ggacaagggc gcctccgccc
     9001 agtcgttcat cgagcgcatg accgccttcg acaagaacct cccgaacgag aaggtcctgc
     9061 ccaagcactc cctgctctac gagtacttca ccgtgtacaa cgagctgacc aaggtcaagt
     9121 acgtgaccga gggcatgcgg aagccggcct tcctgtcggg cgagcagaag aaggcgatcg
     9181 tcgacctgct cttcaagacc aaccgcaagg tcaccgtgaa gcagctgaag gaggactact
     9241 tcaagaagat cgagtgcttc gactccgtcg agatcagcgg cgtggaggac cgcttcaacg
     9301 cctccctggg cacctaccac gacctgctca agatcatcaa ggacaaggac ttcctcgaca
     9361 acgaggagaa cgaggacatc ctggaggaca tcgtcctcac cctgaccctc ttcgaggacc
     9421 gcgagatgat cgaggagcgg ctcaagacct acgcccacct gttcgacgac aaggtgatga
     9481 agcagctgaa gcggcgccgg tacaccggct ggggcgccct ctcccggaag ctgatcaacg
     9541 gcatccggga caagcagagc ggcaagacca tcctggactt cctcaagtcc gacggcttcg
     9601 ccaaccgcgc gttcgccgcg ctcatcgccg acgacagcct gaccttcaag gaggacatcc
     9661 agaaggccca ggtctcgggc cagggcgaca gcctccacga gcacatcgcc aacctggcgg
     9721 gctccccggc gatcaagaag ggcatcctcc agaccgtcaa ggtcgtggac gagctggtca
     9781 aggtgatggg ccgccacaag cccgagaaca tcgtgatcga gatggcccgg gagaaccaga
     9841 ccacccagaa gggccagaag aactcgcgcg agcggatgaa gcggatcgag gagggcatca
     9901 aggagctggg cagccagatc ctgaaggagc acccggtcga gaacacccag ctccagaacg
     9961 agaagctgta cctctactac ctccagaacg gccgcgacat gtacgtggac caggagctgg
    10021 acatcaaccg gctgtccgac tacgacgtcg accacatcgt gccgcagtcc ttcctgaagg
    10081 acgactcgat cgacaacaag gtcctgaccc gctcggacaa gaaccggggc aagtccgaca
    10141 acgtgccctc ggaggaggtc gtgaagaaga tgaagaacta ctggcgccag ctgctcaacg
    10201 ccaagctcat cacccagcgc aagttcgaca acctgaccaa ggccgagcgg ggcggcctga
    10261 gcgagctgga caaggcgggc ttcatcaagc gccagctggt cgagacccgg gcgatcacca
    10321 agcacgtggc ccagatcctg gactcccgga tgaacaccaa gtacgacgag aacgacaagc
    10381 tgatccgcga ggtcaaggtg atcaccctca agagcaagct ggtctccgac ttccgcaagg
    10441 acttccagtt ctacaaggtc cgggagatca acaactacca ccacgcccac gacgcgtacc
    10501 tgaacgccgt cgtgggcacc gcgctgatca agaagtaccc gaagctggag tccgagttcg
    10561 tctacggcga ctacaaggtc tacgacgtgc gcaagatgat cgccaagagc gagcaggaga
    10621 tcggcaaggc caccgcgaag tacttcttct actccaacat catgaacttc ttcaagaccg
    10681 agatcaccct ggccaacggc gagatccgca agcggcccct gatcgagacc aacggcgaga
    10741 ccggcgagat cgtctgggac aagggccgcg acttcgccac cgtccggaag gtgctgtcga
    10801 tgccgcaggt caacatcgtg aagaagaccg aggtgcagac cggcggcttc agcaaggagt
    10861 ccatccggcc caagcgcaac agcgacaagc tgatcgcccg gaagaaggac tgggacccga
    10921 agaagtacgg cggcttcgtc agccccaccg tcgcctactc cgtgctggtc gtggcgaagg
    10981 tcgagaaggg caagagcaag aagctgaagt ccgtgaagga gctgctcggc atcaccatca
    11041 tggagcgctc ctcgttcgag aagaacccga tcgacttcct ggaggccaag ggctacaagg
    11101 aggtcaagaa ggacctcatc atcaagctgc ccaagtacag cctgttcgag ctggagaacg
    11161 gccgcaagcg gatgctcgcc tccgcgcggt tcctgcaaaa gggcaacgag ctggccctcc
    11221 cgtcgaagta cgtcaacttc ctgtacctcg cgtcccacta cgagaagctg aagggctcgc
    11281 ccgaggacaa cgagcagaag cagctcttcg tggagcagca caagcactac ctggacgaga
    11341 tcatcgagca gatcagcgag ttcagcaagc gcgtcatcct ggccgacgcg aacctcgaca
    11401 aggtgctgtc cgcctacaac aagcaccgcg acaagccgat ccgggagcag gcggagaaca
    11461 tcatccacct gttcaccctc accaacctgg gtgccccgcg ggccttcaag tacttcgaca
    11521 ccaccatcga ccgcaaggtc taccgctcca ccaaggaggt cctcgacgcg accctgatcc
    11581 accagagcat caccggcctg tacgagaccc gcatcgacct gagccagctg ggcggcgact
    11641 cgggcggctc caccaacctg tcggacatca tcgagaagga gaccggcaag cagctggtga
    11701 tccaggagtc catcctgatg ctgccggagg aagtggagga agtgatcggc aacaagccgg
    11761 agtcggacat cctggtgcac accgcctacg acgagtcgac cgacgagaac gtcatgctgc
    11821 tgacctcgga cgccccggag tacaagccgt gggccctggt catccaggac tccaacggcg
    11881 agaacaagat caagatgctg tgagaattca gatctacgcg ttccccgcaa aagcggcctt
    11941 tgactccctg caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt
    12001 cattgtcggc gcaactatcg gtatcaagct gtttaagaaa ttcacctcga aagcaagctg
    12061 ataaaccgat acaattaaag gctccttttg gagccttttt ttaagcttgc atgcctgcag
    12121 gtcgacggat cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
    12181 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
    12241 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
    12301 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
    12361 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
    12421 ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
    12481 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
    12541 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
    12601 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
    12661 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
    12721 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
    12781 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
    12841 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
    12901 accgatcccc ggggacctgc aggtcgactc tagagtttta gagctagaaa tagcaagtta
    12961 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc taaaaaaaaa
    13021 ccccgcccct gacagggcgg ggtttttttt gtagcctcag gagttgtagc gtgcacgcgg
    13081 tcgatcttga cggctggcga gaggtgcggg gaggatctga ccgacgcggt ccacacgtgg
    13141 caccgcgatg ctgttgtggg cacaatcgtg ccggttggta ggatccagct agctatttac
    13201 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
    13261 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
    13321 cgtcgcggtg agttcaggct ttttcatatc tcattgcccc cggacgagcg tctgctccgc
    13381 cattcgccgt ccgccgtgcc aatcggatca gccgtccaaa tgcgggattt tcgttagtcg
    13441 gaggccaaac ggcattgagc gtcagcatat catcagcgag ctgaagaaag acaatccccg
    13501 atccgctcca cgtgttgccc cagcaatcag cgcgaccttg cccctccaac gtcatctcgt
    13561 tctccgctca tgagc
//
