LOCUS       sequence-433318-        6175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by
            expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank 
            ID: AFV08795)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6175)
  TITLE     Plasmid-based production of noro, rota, and enterovirus-like 
            particles in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOET5 GII.4 Sydney noro-VLP
FEATURES             Location/Qualifiers
     source          1..6175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             949..1073
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     promoter        complement(1616..1725)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1854..1945
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1870..1887
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     3'UTR           3594..3966
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(4318..4335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4428..5016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4508..4527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5187..5978,5979..6047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5810..5829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6048..6152)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
      421 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
      481 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
      541 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
      601 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
      661 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
      721 atgtcgtgga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
      781 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
      841 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
      901 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
      961 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtggacaagc
     1021 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     1081 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     1141 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     1201 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     1261 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     1321 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     1381 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     1441 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     1501 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc ctctagaata
     1561 ctgcagtgag cggccgccaa aggcctgttg agctctatga attctagaga tctgtgattg
     1621 taaataaaat gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa
     1681 ttcttattta actataatat attgtgttgg gttgaattaa aggtcccggc atcctcaaat
     1741 gcataatttc atagtccccc ttgttgtaag tgatgcgtat ttctgaatct ttgtaaaata
     1801 gcacacaaga ctccaacgcg tttggcgttt attttcttgc tcgactctag gatatcatgg
     1861 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag
     1921 ttttgtaata aaaaaaccta taaatatagg atccatgaag atggcttcca gcgacgctaa
     1981 cccttccgac ggttctgctg ctaacctggt gcctgaagtg aacaacgaag tgatggctct
     2041 ggaacccgtc gtgggtgctg ctattgctgc tcctgtggct ggacagcaga acgtgatcga
     2101 cccctggatt cgtaacaact tcgtgcaggc tcccggtggc gagttcactg ttagtcctag
     2161 aaacgctccc ggcgagatcc tttggagtgc tcctttgggt cccgacttga acccctacct
     2221 gtctcacctg gctcgtatgt acaacggtta cgctggtggt ttcgaggtgc aagtgatcct
     2281 ggctggaaac gctttcaccg ctggcaaagt gatcttcgct gctgtgcctc ctaacttccc
     2341 taccgaggga ctgtccccta gccaagtgac tatgttccct cacatcgtgg tggacgtgcg
     2401 tcagttggaa cctgtgctga tccctctgcc tgacgtgcgc aacaacttct accactacaa
     2461 ccagtctaac gaccccacca tcaagctgat cgctatgctg tacacccctc tgcgtgctaa
     2521 caacgctggc gacgacgtgt tcactgtgtc ctgcagagtg ctgacccgtc cttctcctga
     2581 cttcgacttc atcttcctgg tgccacctac cgtcgagtcc cgtaccaagc ctttctctgt
     2641 gcctgtcctg accgtggaag agatgaccaa ctctcgtttc cccattccgc tggaaaagtt
     2701 gttcaccggt ccatcctccg ctttcgtggt gcaacctcag aacggtcgtt gcactactga
     2761 cggtgtcctg ctgggaacta cccagctgtc tcctgtgaac atctgcacct tcagaggcga
     2821 cgtgacccac atcaccggtt ctcgtaacta caccatgaac ctggcttccc agaactggaa
     2881 cgactacgac cctaccgaag agatccccgc tcctctgggt actccagact tcgtgggaaa
     2941 gatccagggt gtcctgactc agaccactcg taccgatggt tccaccaggg gtcacaaggc
     3001 taccgtgtac actggttccg ctgacttcgc tcctaagctg ggtcgtgtgc agttcgaaac
     3061 cgacaccgac cgtgacttcg aggctaacca gaacaccaag ttcacccctg tgggtgtcat
     3121 ccaggacggt ggtactactc accgtaacga gcctcagcag tgggtcctgc cttcctactc
     3181 tggtcgtaac acccacaacg tgcacttggc tccagctgtg gctccaactt tcccaggcga
     3241 gcaactgctg ttcttccgtt ccaccatgcc tggttgctct ggttacccca acatggacct
     3301 ggactgcctg ctgcctcaag agtgggtgca gtacttctat caagaggctg ctcccgctca
     3361 gtctgatgtg gctctgttgc gtttcgtgaa ccccgacacc ggtcgtgtct tgttcgagtg
     3421 caagctgcac aagtccggtt acgtgaccgt ggctcacact ggtcaacacg acctggtcat
     3481 ccctccaaac ggttacttcc gtttcgactc ctgggtcaac cagttctaca ccctggctcc
     3541 tatgggcaac ggaactggta gacgtcgtgc tgtgtaataa agcttattaa taaaacacga
     3601 tacattgtta ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca
     3661 attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag
     3721 cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag
     3781 atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg
     3841 gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc
     3901 tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa
     3961 tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa
     4021 acacgttaaa taacgcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat
     4081 tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag
     4141 ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc
     4201 tttttgggcc tcatgggcct tccgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4261 ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc
     4321 gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa
     4381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga accacgctca
     5341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
