LOCUS       sequence-433323-        5775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Forms rotavirus-like particles in insect cells by expression of VP6 
            (GenBank ID: ACL93331.1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5775)
  TITLE     Plasmid-based production of noro, rota, and enterovirus-like 
            particles in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOET5 rota-VLP
FEATURES             Location/Qualifiers
     source          1..5775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1841..1858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           2210..2582
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     regulatory      3796..3805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3848..3939)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4069..4160
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             complement(4703..4827)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5437..5456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5438..5456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5467..5484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5467..5483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5476..5498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
     1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
     1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggccc aaaaagctca
     1981 actacaaatt tgatcgcgga cgtgttagcc gacacaatta ataggcgtcg tgtggctatg
     2041 gcaaaatcgt cttcggaagc aacttctaac gacgagggtt gggacgacga cgataatcgg
     2101 cctaataaag ctaacacgcc cgatgttaaa tatgtccaag cgttatttaa cgtgtttacg
     2161 tcgagtcaat tgtacactaa cgacagtgat gaaagaaata caaaagcgca taatattttg
     2221 aacgacgtcg aacctttatt acaaaacaaa acacaaacga atatcgacaa agctagattg
     2281 ctgctacaag atttggcaag ttttgtggcg ttgagcgaaa atccattaga tagtccagcc
     2341 atcggttcgg aaaaacaacc cttgtttgaa actaatcgaa acctatttta caaatctatt
     2401 gaggatttaa tatttaaatt cagatataaa gacgctgaaa atcatttgat tttcgctcta
     2461 acataccacc ctaaagatta taaatttaat gaattattaa aatacgtaca acaattgtct
     2521 gtaaatcaac aacgcacaga atctagcgct taataaatgt actaataaca atgtatcgtg
     2581 ttttattaat aagctttaag gtaccttact tgatgagcat agaacgaatg gaagcgactg
     2641 tgaatactct ttgaaggttg tcctcacgtg aggggctgta gttggtaatg agttctgtcc
     2701 agttcatgcc aggtgggaat actggaccaa cggggatggc gtactcctga cgcacggcag
     2761 tgacgttagc aagcaaggtt tcgttggcat cagcgagcac agactcacag acagcacttt
     2821 cgattctgag agtcagaccc acggtggcat ggtgttggaa aggttgagcc tgtgggaaca
     2881 gggcgttgac agctggagtc atgttgggag ggcgcataag ctggaaagac aagcggatgg
     2941 tgtcgaagtt cctggcaatg atggttccga acctagcttg gtatgtgtta atgatctggc
     3001 cgttgagcag gaactctact tcaacgttgt taggacgcag aatgatgggg ttgaagaacc
     3061 aggttgtggc accatcagca ctgttaatga cacgagggaa ggagaatctc tcggcgtctg
     3121 gaagcaaggt gattgtggca gtggtgagag cgcgcctcag ttgtacaatg tgttcgaatt
     3181 gctggatgtt ggcaggagcg ttgagggcgc aagagtaatc gaagccagca acctggatct
     3241 ctgaaccggc gttaagccac atagttccca tcaagttgtc atgcataggc tggctacggt
     3301 tcaaagtgaa agaagcactg taggggaaga tgttaggttt gtggaaaacg aaaccggttc
     3361 tttgacgtct gttctggagg ttccagttct cgatgtattc gctagagtta tcgaagttaa
     3421 tacgtttgaa cttgatgcca gccagctttc tcaaggcttc gctttgtgga gccacaccgt
     3481 tacgctgaga ctctctagcc atttcgtcca tgcagacgtt atcaatgaag tcgatgaagt
     3541 actcgatggt tgtgcgagca gtttccacgt agttggcatc aaggttaagc aaagtagtac
     3601 cgagcagtcc gaagtcgaag gtccagttcc taatgggcaa gttaccgatg ccacctgttt
     3661 ggaagtcgtt tccgttcata gtgacaatca tctggttgaa ttgctggatc agatcggata
     3721 cgttagagta gagagtgcct tcaacaatct tgtccctggc gtccttcaaa gttttagaga
     3781 gtgagtagag tacctccatg gtggcggttt tttaggagtt ctcgagagag tcgactgtgg
     3841 atcctatatt tataggtttt tttattacaa aactgttacg aaaacagtaa aatacttatt
     3901 tatttgcgag atggttatca ttttaattat ctccatgata tcctagagtc gagcaagaaa
     3961 ataaaacgcc aaacgcgttg gagtcttgtg tgctatttta caaagattca gaaatacgca
     4021 tcacttacaa caagggggac tatgaaatta tgcatttgag gatgccggat catggagata
     4081 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4141 taataaaaaa acctataaat acagatctct agaattcaac tcctaaaaaa ccgccaccgc
     4201 ggccgctcac tgcagtattc tagaggcaac atattgtaca aaaccgacga tcccaaattc
     4261 attgattata taaatttaat aattaaagca acacactccg aagaactacc agaaaatagc
     4321 actgttgtaa attacagaaa aactatgcgc agcggtacta tacaccccat taaaaaagac
     4381 atatatattt atgacaacaa aaaatttact ctatacgata gatacatata tggatacgat
     4441 aataactatg ttaattttta tgaggagaaa aatgaaaaag agaaggaata cgaagaagaa
     4501 gacgacaagg cgtctagttt atgtgaaaat aaaattatat tgtcgcaaat taactgtgaa
     4561 tcatttgaaa atgattttaa atattacctc agcgattata actacgcgtt ttcaattata
     4621 gataatacta caaatgttct tgttgcgttt ggtttgtatc gttaataaaa aacaaattta
     4681 gcatttataa ttgttttatt attcaataat tacaaatagg attgagaccc ttgcagttgc
     4741 cagcaaacgg acagagcttg tccacagttt tcattttttg gcagtttggt ggtttataca
     4801 catcttggga tttgattaaa agatgcatga cttcacccac acacttggag tcgtggttgt
     4861 agaaatgcgt cagagccttg attaaacatc ggttttcaca cttggcacac gaaaccacgt
     4921 ttgcaatgta acagtttagt ataaacctct tcctgtacat accgccgcgc ggtacattgt
     4981 tttctttcaa atcgttcaat atttttttta tacacggagg catattaatc ttgttgttca
     5041 gcatgtccac gacatttttc tttccttcta cgcattcttt catacaaatg tttctcttgc
     5101 tgcgtttttt aaaattggaa tttttaattt cattaatagt gggcggagcc gttaacagca
     5161 tgtacaatcg cagtccggaa attttgacca aaaccccgcc attgtagaat accgtgtatg
     5221 gagttagggt caatttatca ataaaatcat cgggatcaat taaatatgta gctttcttgt
     5281 ctaaacatga cgctctaatg agcggactcc acacgttata cgatgcattc gccatacttc
     5341 gcggcttctc gcaccagcgg tcctgattcg actaacggtc gattggatgg cttaatagtt
     5401 ttaacgtcac ggccttgacg gccttccgcc aattcgccct atagtgagtc gtattacgtc
     5461 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5521 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5581 cgaaacgccc ttcccaacag ttgcgcagcc tgaatggcga atgggagcgc cctgtagcgg
     5641 ccactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5701 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5761 acgcttacaa tttag
//
