LOCUS       sequence-43334-e       10543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS272
FEATURES             Location/Qualifiers
     source          1..10543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     403..419
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     403..419
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1610..2323
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1640..1668)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2263..2283
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2802..3049
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2818..2836)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(3062..3095)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(3153..4509)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(3153..3350)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3356..4165)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4079..4098
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4166..4509)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(4564..4597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4633..4652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4634..4652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4662..4679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4662..4678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4671..4693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4823..5278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4910..4929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5120..5141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5578..5982
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5995..7089
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7582..7601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7701..7723
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7761..7779)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7821..8324
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8361..8465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8466..8534,8535..9326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8684..8703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9497..10085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9986..10005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10239..10256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(10409..10414,10415..10432,10433..10439)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10447..10463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10452..10474
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10471..10487
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10471..10487
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10506..10526
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10508..10526
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc
       61 atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct
      121 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt
      181 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa
      241 tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg
      301 gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt
      361 cttgctcatt agaaagaaag catagcaatc taatctaagt tttctagaac tagtggatcc
      421 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacaatgt tgtctttcac
      481 aaccaagaat agtttccgct tattactttt aatactgtca tgcatatcga cgatacgcgc
      541 acaatttttc gtgcaatcat catcttcgaa ttcttcagca gtatctactg cacgttcttc
      601 cgtaagtaga gttagttctt caagttccat tttgtcatcc agtatggttt cttcctcaag
      661 tgctgactca tcttccctta cttcatcgac atcaagtagg tccctcgtgt cacatacgag
      721 ttcgtctacc agcattgcct ccatatcgtt cacatcattc agtttctcat cagattcaag
      781 caccagcagc tcttcctctg cttcttcaga ttcttcatcc tcctcatcct tttccatctc
      841 ttcgacatcc gcaacttctg aatcatcgac gtcttctacg caaacgtcaa catcatcttc
      901 atcctcactg tcgtcaacgc cgtcctcttc atcatcccca tcaacaatca cttctgcacc
      961 ttcaacctcc tccacaccat ccactactgc ctataatcaa ggaagcacta tcaccagtat
     1021 tattaacggt aagacgattc tctcaaatca ttatactacc gttacgtaca caccatcagc
     1081 aaccgctgat tcaagcaata aatccaaaag ttcgggtctt tctaaaaaaa atagaaacat
     1141 cgtcattggt tgtgtggttg gtattggtgt accactgatt ctggtgatac tggctttaat
     1201 ttacatgttt tgtatccaat catcaaggac ggactttatt gactcagatg gtaaggtcgt
     1261 tacggcatat agagctaaca aatttacgaa atggtggtat atgcttttgg gtaagaaagt
     1321 tagtgatgaa taccactccg attcaccatt aggtggtagt gcatcgtccg caggtggttt
     1381 agacctagat gaagcagatg atgtgatgga gcaaagctcc cttttcgacg tcagaataag
     1441 agatagcgat agcgtattac caaacgccaa caccgccgac cataataata ccaatagcgg
     1501 tggagagcct ataaatagta gcgttgcgtc aaatgacata atagaagaaa aattctatga
     1561 tgaacaaggt aacgaattat caccacgaaa ttatgcgtcg acctcgacaa tgagtaaagg
     1621 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1681 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1741 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1801 cacttatggt gttcaatgct tttcaagata cccagatcat atgaaacagc atgacttttt
     1861 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1921 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1981 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     2041 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     2101 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     2161 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     2221 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     2281 aacagctgct gggattacac atggcatgga tgaactatac aaaggtggat ctggaggttc
     2341 aggtggaagt cctaggttgg ctgctgcact tgaacgtatt gagaagaact ttgtcattac
     2401 tgacccaaga ttgccagata atcccattat attcgcgtcc gatagtttct tgcagttgac
     2461 agaatatagc cgtgaagaaa ttttgggaag aaactgcagg tttctacaag gtcctgaaac
     2521 tgatcgcgcg acagtgagaa aaattagaga tgccatagat aaccaaacag aggtcactgt
     2581 tcagctgatt aattatacaa agagtggtaa aaagttctgg aacctctttc acttgcagcc
     2641 tatgcgagat cagaagggag atgtccagta ctttattggg gttcagttgg atggaactga
     2701 gcatgtccga gatgctgccg agagagaggc agtcatgctg attaagaaaa ctgcagaaga
     2761 gattgataaa gcggtggata cctgggtgta agcttctcga gtcatgtaat tagttatgtc
     2821 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     2881 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     2941 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     3001 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtacct
     3061 aataacttcg tatagcatac attatacgaa gttatattaa gggttctcga cgttttcgac
     3121 actggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
     3181 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
     3241 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
     3301 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgattaga
     3361 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     3421 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     3481 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     3541 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     3601 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     3661 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     3721 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     3781 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3841 ctaatacctg gaatgctgtt ttgccgggga tcgcagtggt gagtaaccat gcatcatcag
     3901 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3961 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     4021 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     4081 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     4141 aaacgtgagt cttttcctta cccatggttg tttatgttcg gatgtgatgt gagaactgta
     4201 tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca aatcctaacc
     4261 ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg tgaggggagc
     4321 gtttccctgc tcgcaggtct gcagcgagga gccgtaattt ttgcttcgcg ccgtgcggcc
     4381 atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa tgtacgggcg
     4441 acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg tattctgggc
     4501 ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaacag atctctagac
     4561 ctaataactt cgtatagcat acattatacg aagttatatt aagggttgtc ttaattaagg
     4621 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4681 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4741 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4801 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4861 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4921 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4981 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5041 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5101 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5161 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5221 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
     5281 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgacggtcg
     5341 aggagaactt ctagtatatc cacataccta atattattgc cttattaaaa atggaatccc
     5401 aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact tccttgttca
     5461 tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca acaagtaatt
     5521 ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac cgcagttaac
     5581 tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt
     5641 ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac
     5701 tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa
     5761 ttgggagaaa aaggaaaggt gagaggccgg aaccggcttt tcatatagaa tagagaagcg
     5821 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     5881 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     5941 tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc ccctatgtct
     6001 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa
     6061 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa
     6121 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg tcccacttcc agatgaggcg
     6181 ctggaagcct ccaagaaggt tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg
     6241 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg
     6301 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc
     6361 aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt
     6421 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac
     6481 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag
     6541 ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg
     6601 agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa
     6661 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata
     6721 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc
     6781 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt
     6841 ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt tgaccctatc
     6901 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt
     6961 aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta
     7021 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
     7081 cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt
     7141 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
     7201 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatagtaa aggaatacag
     7261 gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt
     7321 aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac
     7381 tacgattcct aatttgatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa
     7441 aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt
     7501 cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttacgacgta
     7561 gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     7621 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7681 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7741 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7801 aataatggtt tcttaggacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta
     7861 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga
     7921 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa
     7981 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag
     8041 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc
     8101 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga
     8161 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga
     8221 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt
     8281 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg
     8341 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8401 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8461 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8521 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8581 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8641 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8701 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8761 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8821 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8881 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8941 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9001 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9061 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9121 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9181 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9241 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9301 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9361 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9421 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9481 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9601 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     9661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10021 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10081 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10141 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10201 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10261 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10321 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10381 gttacctcac tcattaggca ccccaggctt tacactttat gcttccggct cctatgttgt
    10441 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    10501 agcgcgcaat taaccctcac taaagggaac aaaagctgga gct
//
