LOCUS       sequence-433354-        9651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR
  TITLE     Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated 
            by Multisite Phosphorylation.
  JOURNAL   J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13.
  PUBMED    21669867
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-08-12"; volume: "286(32)"; pages: "28066-79. 
            Epub 2011 Jun 13"
COMMENT     Sequence Label: Lentiviral CaMKKbeta
FEATURES             Location/Qualifiers
     source          1..9651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     162..184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     216..236
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        218..236
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        264..490
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             491..671
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    718..843
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1336..1569
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1754..1798
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2065..2182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2228..2738
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2657..2675
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2762..2785
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4443..5017,5018..5020,5021..5029)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4610..4627)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4837..4856
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5030..5032,5033..5035,5036..5749)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5030..5032,5033..5035,5036..5746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5336..5355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5683..5704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5758..6346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5811..5831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6229..6240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6433..6666
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6738..6859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6775..6794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6829..6848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6899..7034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7054..7073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7055..7073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7083..7100)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7083..7099)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7092..7114)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7241..7696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7328..7347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7538..7559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7722..7826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7827..7895,7896..8687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8045..8064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8858..9446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9347..9366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9600..9617
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
      241 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      301 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      361 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      421 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      481 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      541 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      601 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      661 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      721 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      781 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      841 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
      901 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      961 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1021 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1081 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1141 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1201 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1261 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1321 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1381 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1441 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1501 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1561 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1621 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1681 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1741 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1801 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1861 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1921 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     1981 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2041 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     2101 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2161 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga
     2221 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     2281 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     2341 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     2401 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     2461 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     2521 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     2581 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     2641 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     2701 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg gtgctagcat
     2761 ggactacaag gacgacgatg acaaagctag ctcatcatgt gtctctagcc agcccaccag
     2821 cgaccgggtg gccccccagg atgagctggg aagtgggggt ggcagccggg aaggccagaa
     2881 gccctgtgag gcactgcggg gactctcatc cttaagtatc cacttgggca tggaatcctt
     2941 catcgtggtc accgagtgtg agccaggccg gggtgtggac ctcaacctgg ccagagacca
     3001 gcctccggag gccgatggcc aggaactccc cctcgaagcc tcggaccctg agtcccggtc
     3061 cccgctttct ggtcgcaaga tgtccctgca ggagccgtcc cagggcgggc cggcatccag
     3121 cagcaacagc ctggacatga atggacgctg catctgccca tccctgtcct actcaccagc
     3181 cagctcccca cagtcctctc cccggatgcc ccggcggccc acagtagagt cacaccacgt
     3241 ctccattacc ggtttgcagg actgcgtgca gctgaatcag tacaccctga aggatgaaat
     3301 tggaaagggc tcctatggtg ttgtcaagct ggcctacaat gaaaatgaca atacttatta
     3361 cgcaatgaaa gtgctgtcca aaaagaagct gatccgacag gctggcttcc cacgtcgccc
     3421 cccaccacga ggagctcgcc cagccccagg gggctgcatc cagcccaggg gccccatcga
     3481 gcaagtgtac caggagattg ctatcctcaa aaagctggat catcccaacg tggtgaagct
     3541 ggtagaggtc ctggatgacc ctaatgagga ccatctgtac atggtgtttg agttggtgaa
     3601 ccaagggcct gtaatggaag tgcccaccct caagccactg tccgaagacc aggcccgctt
     3661 ctacttccag gatctgatca aaggcataga gtacttgcat taccagaaga tcattcaccg
     3721 ggccatcaaa ccctccaacc tcctggtggg ggaagacggg cacatcaaga ttgccgactt
     3781 cggtgtaagc aacgagttca agggcagcga cgccttgctg tctaacaccg tgggcacacc
     3841 tgccttcatg gcacccgagt cgctctcaga gacccgcaag atcttctccg gaaaggcgtt
     3901 ggatgtttgg gccatgggtg tgacgctgta ctgctttgtc tttggccagt gccctttcat
     3961 ggatgaacga atcatgtgtt tgcacagtaa gatcaagagt caggccctgg agtttcccga
     4021 ccagcccgat atagccgaag acttgaaaga tctgatcact cggatgttgg acaaaaatcc
     4081 agagtccagg attgtggtgc ctgaaatcaa gctgcaccct tgggttacga ggcacggggc
     4141 cgagccattg ccgtcggagg acgagaactg cacactggtc gaggtgaccg aagaggaggt
     4201 cgagaattca gtcaaacaca ttcccagcct ggcaactgtg atcctggtaa agaccatgat
     4261 tcgaaagcgc tcattcggga acccgttcga aggtagccgc cgggaggaac gttccctgtc
     4321 agcgcctgga aacctgctca cgaagcaagg cagcgaagac agcccccggg gcccagagcc
     4381 ggcccctgtg ggggaggagg aagtgctctt gtgagtcgac ggtaccgcgg gcccgggatc
     4441 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4501 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4561 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4621 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4681 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     4741 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     4801 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4861 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4921 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     4981 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
     5041 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5101 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5161 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5221 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5281 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5341 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5401 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5461 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5521 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5581 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5641 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5701 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag attcgacaat
     5761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6121 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     6181 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6301 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct
     6361 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6421 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6661 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6721 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6781 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6841 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6961 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7021 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7081 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7141 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7201 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7441 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7501 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7561 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7621 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7681 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7801 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7861 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7921 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7981 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8041 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8101 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8161 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8221 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8281 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8341 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8401 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8461 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8521 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8581 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8641 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8701 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8761 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8821 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8881 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8941 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9001 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9061 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9121 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9181 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9241 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9301 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9361 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9421 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9481 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9541 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9601 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct c
//
