LOCUS       sequence-433367-       10597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual-enSERT-1 eGFP vector for site-specific integration into the H11
            locus (contains hsp68 minimal promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Hollingsworth EW, Liu TA, Alcantara JA, Chen CX, Jacinto SH, Kvon EZ
  TITLE     Rapid and quantitative functional interrogation of human enhancer 
            variant activity in live mice.
  JOURNAL   Nat Commun. 2025 Jan 6;16(1):409. doi: 10.1038/s41467-024-55500-7.
  PUBMED    39762235
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-6"; volume: "16(1):409. doi"; pages: " 
            10.1038/s41467-024-55500-7"
COMMENT     Sequence Label: PCR4-Hsp68::eGFP-H11
FEATURES             Location/Qualifiers
     source          1..10597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(97..165,166..957)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(315..334)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1207..2001)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1207..2001)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1318..1337)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1928..1947
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2350..2652)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2452..2471)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     2775..2797
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2789..2806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2790..2806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2813..2832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2813..2831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6129..6138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6135..6137,6138..6140,6141..6854)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6135..6137,6138..6140,6141..6851)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6180..6201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6441..6460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6788..6809
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7009..7143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7046..7065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7100..7119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7140..7156)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7156)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9541..9559)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9580..9596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9580..9596)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9593..9615)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9604..9620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9628..9634,9635..9652,9653..9658))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9673..9694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9811..9828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9982..10570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10062..10081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag acaataaccc
       61 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      121 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      181 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      241 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      301 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      361 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      421 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      481 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      541 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      601 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      661 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
      721 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
      781 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
      841 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
      901 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
      961 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1021 aggatctagg tgaagatcct ttttgataat ctcatgagcg gatacatatt tgaatgtatt
     1081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1201 aataattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     1261 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     1321 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     1381 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     1441 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     1501 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     1561 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     1621 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     1681 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     1741 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     1801 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     1861 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     1921 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     1981 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagagcttga
     2041 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     2101 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     2161 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     2221 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     2281 ctgcggactg gctttctacg tgaaaaggat ctaggtgaag atcctttttg ataatctcat
     2341 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
     2401 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
     2461 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
     2521 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc ccggcgtgtc
     2581 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
     2641 aaccttaaac tgccgtacgt ataggctgcg caactgttgg gaagggcgat cggtgcgggc
     2701 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     2761 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     2821 ctcactatag ggcgaattga atttagccct ggtacatgta tggtggcaag tgttcaccac
     2881 atccacctcc ttgtaagtat ccaaatcaac aaaagtagta aactttgaca tttttaggaa
     2941 catctacaac tcatgagttt cttctttctc cccatcaggt gtgcttcctc ctgggataga
     3001 catggctcct ttacaggggc tatctggccc actcctgggt caacccttgt accctttggt
     3061 ctctgctgct agtcaccctc tcctaaaccc tcgtcctggg acccctctgc atttggcggt
     3121 gatgcagcag cagctacagc gctcaggtaa ggtttagaaa taggccaggt tagctgatca
     3181 cactggaaag ttctgagatt tccaggagca atagttatca ctgccccttt gtttcttagg
     3241 tgtcactaga aaaacaggtg aagtgaactg acctgtgcag tctcagggat tattactact
     3301 gcaaggacag tcggagccag ccatgctcta gctctgtttt tcacttctta taagcctgag
     3361 aatttttgct gagatgtgaa catgtcagcc ttggtgggct ttcttacgtt gcattttctt
     3421 acgttgcatt ttgcacttta ggaagccttt ggttaaatcc tctggtagac ttcccatccc
     3481 ccaaagtaac aaaccattca agcaaagtag gtcagaaaat attgtcaata ctgagaacag
     3541 gacttgggag tgagtatcca agggtggtct tagtagactt gtggggttct gtgggtgagg
     3601 tgtagagtag agctactttg ctgcactgac actcttctct ccatagttct gcatcctcca
     3661 ggctctagtt cccaggcagc agctatcagc gttcagactc ctcagaatgt acccagccgg
     3721 tcgggcatgc cccacatgca ctcccagctg gagcatcgta ccagccaaag gagcagctcc
     3781 cctgtgggcc ttgccaaatg gtttggctca gatgtgctac agcagcctct gccctccatg
     3841 cccaccaaag tcatcagtgt agatgaactg gaatatagac agtgaagagg gcaggctggc
     3901 tcacccatac ctggacctgt ggtgacaccc tggtcatgac tctcattccc tctttgtaat
     3961 gggcttttac attggagcac actatgtgaa gatgtttagg ggatccacat acctaccatg
     4021 atctacatta tgacagaagg ctgttaaatc gaatgaacct acatggttca aatacaaggg
     4081 atacaagatt gtcagtcctg gaagtctttc ttttataaaa tatgtgaatg aagtgttggt
     4141 gtcttctaga ggtgacacct aagggttctg aaaaaataaa atgtatagac ccttatgtac
     4201 agacctgtgt ataaactttt gtacatacaa atagggtagc tttttttgaa cttatacata
     4261 cagctgtaca taaagtaact atcagttagg cttgtgtcaa ctgtttggat ttttttcact
     4321 tgaatatttg ggactttttc ttttggttta ttaaaagtta catatgccac gtgtgtgaac
     4381 gatatggctg gtactgtgtt tatttcttcc atgaactaag acagtctaaa tgagttcctt
     4441 tcacgtttta attttacctt aggacttctg gaatttcttc tgcacataaa gttctgatag
     4501 cattagttta agctggacta accctgaaag tagcttgtgg caagtatcaa ggaatcaata
     4561 ttatactcta caaaatcaaa gtttacagag aagtcatata gtaatttttc tgaaatttac
     4621 tggcacaatg ttaatccagc ctgactccaa ctaattaatg gtcacattaa tttaagtctt
     4681 tcccttgcct ctgctgcatt agtttctctc aaaattgtta acttacaact tgaagtctgg
     4741 tattataaat tgaatgtaaa gcattctgaa agatactata ctgattgcag gtttttcagt
     4801 caggttcaag ctaatttgac cagtcattgg attaattatg gatctggggc cataaatgct
     4861 attttaattc cactatagag attaaaataa gccattctcc atttcataat attctattgg
     4921 actttgactg caggggcctc caagtcttga cagtagatta taatccttca gctgcccact
     4981 ctactggagg aggacaaact ggtcactttt cagcaaaacc tggctgtgga tcagggcagt
     5041 ctggtacttc caagctcatt agatgccatc atgctctcac tgcctcctca gcttcaagag
     5101 gaatctggaa aaagcagtcc cactggtcag gaaaggaaca ctagtgcact tatcctgggt
     5161 gtctgctgag ccaacagtgg tagtaaggta agggcaggat gtgtcaaact gccaatagag
     5221 aactacttac tcttcaggct gaagctgatg gaacaaccgg tgccaggaac atccaaactg
     5281 agcagccggg gtccccccca ccccccaccc cgcccctccc ggcaactttg agcctgtgct
     5341 gggacagagc ctctagttcc taaattagtc catgaggtca gaggcagcac tgccattgta
     5401 acgcgattgg agaggatcac gtcaccggac acgcccccag gcatctccct gggtctccta
     5461 aacttggcgg ggagaagttt tagcccttaa gttttagcct ttaaccccca tattcagaac
     5521 tgtgcgagtt ggcgaaaccc cacaaatcac aacaaactgt acacaacacc gagctagagg
     5581 tgatctttct tgtccattcc acacaggcct tagtaatgcg tcgccatagc aacagtgtca
     5641 ctagtagcac cagcacttcc ccacaccctc cccctcagga atccgtactc tccagtgaac
     5701 cccagaaacc tctggagagt tctggacaag ggcggaaccc acaactccga ttactcaagg
     5761 gaggcgggga agctccacca gacgcgaaac tgctggaaga ttcctggccc caaggcctcc
     5821 tccggctcgc tgattggccc agcggagagt gggcggggcc ggtgaagact ccttaaaggc
     5881 gcagggcggc gagcaggtca ccagacgctg acagctactc agaaccaaat ctggttccat
     5941 ccagagacaa gcgaagacaa gagaagcaga gcgagcggcg cgttcccgat cctcggccag
     6001 gaccagcctt ccccagagca tccctgccgc ggagcgcaac cttcccagga gcatccctgc
     6061 cgcggagcgc aactttcccc ggagcatcca cgccgcggag cgcagccttc cagaagcaga
     6121 gcgcggcggc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6181 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6301 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6541 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6601 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6661 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6721 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6781 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6841 agctgtacaa gtagtaataa ctagggcagg ggggatctaa gctctagata agtaatgatc
     6901 ataatcagca ctcaccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
     6961 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     7021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7141 atccactagt tctagagcgg caagcagaaa gggaacaagg ggatattaaa tgtgtataga
     7201 cacgctagag agatggctca gcagttaaga gaactagctg gtctttcaga ggtcctgaga
     7261 tcaattttag acacccacat ggtggctcat gaccatctat ctataaatgg atctgatttt
     7321 catgtctggc agtgtacaga agctaactga agaaaggtgg aagacccaca agagttcaag
     7381 ataagcccta tatagtgaag ttcaaggcaa gctttttcta cctgaaactt agtctcaaaa
     7441 aaaaatgaat acgtaaacag tcttccaggg gataagaacc ttacagaaaa agcagaaatg
     7501 cctggggcac tggattaccg atgtaatcaa attcagtcct tgaattgaac acaggattgc
     7561 ctagagcaag gccagccaga gattcatctc agagggagaa aggtgtcttt ggagcaattt
     7621 tgtggtaatc tagtatgtat cacataagtt tagacgcatt tgggactgga aagatgtgaa
     7681 caaagcaccc tatggctcac atctgtcatt aactctagtt ccagtgcacc tgacaccgtc
     7741 ttctggcctc tgcagtgacc aagcacatgg gtagtatgta gacatataca taagcaaaac
     7801 acacatcatt aaaaagtgac atttcccaaa ggaagctgaa gaaccagttc ttgagaagat
     7861 agtagaaatc agaaggggaa atagtagaca tacagaggga ctgaccaggt tgtgtcacct
     7921 ttataggcta ggctaattga tgatcgacac tagcgctctt tgtgaaggac acacaaatga
     7981 gacatagttt ataggactaa acacacttct aagcaattta atgagactta agaccctgtc
     8041 tctagcaaat actctggatg atattcagct caaggctctt gtcagacatg tttccatttt
     8101 caaggtgagc taactggcca aaactgccaa caacctgtag tgaatagaga agatgagaaa
     8161 atctggattc tcaaatgacc taatgaaagc cactggagcg ccatatggtt tctgtgaaaa
     8221 tgccttttca atcattaacc tcttaaatga gtgttagcat cctaactaat gagtggtgca
     8281 gaatagtggg tctgcttagc ttaactaagg ccaagaaaac aaaacaggaa attcattcca
     8341 tgtcatgaga ctcatactac gaggttccct tagacctcag gagaaaaagt ctttggctgt
     8401 aagaacacac ctcagtggat gtggtagact atgcctttac tctttttttt tttttttttt
     8461 tttttgggtt ttttcgagac agggtttctc tgtgtagcct tggctgtcct ggaactcact
     8521 ttgttgacca ggctggcctc gaactcagag atccgcctgc ctctgcctct caagtgctag
     8581 aattaaaagt gtgcgccacc actcccagct atatagacta tgcctttaat caggacactt
     8641 gggaggcagg gggatctcaa attcaattcc agacagatga ctgacaaaca cacacataca
     8701 ccattcgtct tttctttttt tcttttttct tttgaaaaca ggtttctcta tgtagccctg
     8761 gctttcctag aattcaatct atagacccag ctggcctcaa acacagaacc tcttgcctct
     8821 gcctcctcaa cactatgact aaaggtgtga accaccacca ccaccaccca cctggccaaa
     8881 gaatgaattg agtgaataag tacgagtgca actctccagc agcctgaggc aagaaagctt
     8941 aacagttgaa cctgagaccc tacttcgttt gtgttgtaca tatcttctca taatgaacta
     9001 ggcagcctag tctctctacc aaatacacca tgcacttaca cctcctcaaa ccaactgcaa
     9061 cagctacaca catttccatg gcttgcctgt aataataaat tcacaaactt cctctaagta
     9121 acttttagtg gaaatctacc aggttatgcc tatttgtgat tgggtgcaca gatgactaga
     9181 tgagatgtaa tttgggaagt gacatgcaat aggaaaaatg aatatataac ctataaatag
     9241 aaaacaccaa ggcaagcgtg gtgactcaca cctttaatta gcacttggga ggcagaagtc
     9301 gcatcatctc ttaggtcaag gccaatctag ttccagacca gcaaggacaa cagaaacctt
     9361 gccttgatat aaacatttta aaactttaaa aatagagaag cccaaactct gctcattggt
     9421 aatctaccct tcctttcttt agacaccttg agaagctagg ctacatagct ttgggtcctt
     9481 acgtaccccc actcactcag cctgcagtca atgaattcgt ttaaacctgc aggactagtc
     9541 cctttagtga gggttaattc tgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10561 aggatctcaa gaagatcctt tgatcttttc tacgggg
//
