LOCUS       sequence-433380-        8755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of reporter EGFP with a TAG stop codon at 39 aa position;
            can be packaged into bacmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Cao X, Malley K, 
            Huang Z, Pasha M, Anthony J, van Opijnen T, Chatterjee A
  TITLE     Virus-assisted directed evolution of enhanced suppressor tRNAs in 
            mammalian cells.
  JOURNAL   Nat Methods. 2023 Jan;20(1):95-103. doi: 10.1038/s41592-022-01706-w.
            Epub 2022 Dec 22.
  PUBMED    36550276
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01706-w"; journalName: "Nat Methods"; date: 
            "2023-01"; volume: "20"; issue: "1"; pages: "95-103"
COMMENT     Sequence Label: pAcBac1-GFP(39TAG)
FEATURES             Location/Qualifiers
     source          1..8755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3605..3610,3611..3627,3628..3633))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4063..4287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4276..4293
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4342..4930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4448..4459
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4857..4877
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4956..4973)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4989..5018)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(5028..5738,5739..5741,5742..5744))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT*GKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5070..5091)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5419..5438
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5678..5699
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5741..5750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5772..5791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5773..5791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5836..6039)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5865..5885)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6040..6419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6514..6605
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6530..6547
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(6664..6711,6712..8199)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    8345..8479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8382..8401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8436..8455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8508..8673
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3481 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     3541 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     3601 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     3661 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
     3721 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
     3781 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg
     3841 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
     3901 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
     3961 acgtacccgt agtggctatg gcagggcttg cgcttaatgc gccgctacag ggcgcgtggg
     4021 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     4081 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     4141 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     4201 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     4261 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctc
     4321 tagactcgag ttaaagtcga cgcggggagg cggcccaaag ggagatccga ctcgtctgag
     4381 ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt
     4441 ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg
     4501 caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc acggaattgt
     4561 cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg
     4621 ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa
     4681 tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga
     4741 caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa
     4801 tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata
     4861 gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca
     4921 gaggttgatt gtcgacttaa cgcgttgaat tctcaatggt gatggtgatg atgaccggta
     4981 tgcatattca gatcctcttc tgagatgagt ttttgttcga agggcccctt gtacagctcg
     5041 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     5101 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     5161 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     5221 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     5281 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     5341 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     5401 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     5461 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     5521 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     5581 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccctaggt ggcatcgccc
     5641 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     5701 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc ggcgctagcc
     5761 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
     5821 tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     5881 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     5941 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     6001 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     6061 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     6121 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     6181 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     6241 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     6301 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     6361 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     6421 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cggatcctgc aggtatttgc
     6481 ggccgcggtc cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     6541 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     6601 taaatattcc ggattattca taccgtccca ccatcgggcg cgaactccta aaaaaccgcc
     6661 accatgaagt gccttttgta cttagccttt ttattcattg gggtgaattg caagttcacc
     6721 atagtttttc cacacaacca aaaaggaaac tggaaaaatg ttccttctaa ttaccattat
     6781 tgcccgtcaa gctcagattt aaattggcat aatgacttaa taggcacagc cttacaagtc
     6841 aaaatgccca agagtcacaa ggctattcaa gcagacggtt ggatgtgtca tgcttccaaa
     6901 tgggtcacta cttgtgattt ccgctggtat ggaccgaagt atataacaca ttccatccga
     6961 tccttcactc catctgtaga acaatgcaag gaaagcattg aacaaacgaa acaaggaact
     7021 tggctgaatc caggcttccc tcctcaaagt tgtggatatg caactgtgac ggatgccgaa
     7081 gcagtgattg tccaggtgac tcctcaccat gtgctggttg atgaatacac aggagaatgg
     7141 gttgattcac agttcatcaa cggaaaatgc agcaattaca tatgccccac tgtccataac
     7201 tctacaacct ggcattctga ctataaggtc aaagggctat gtgattctaa cctcatttcc
     7261 atggacatca ccttcttctc agaggacgga gagctatcat ccctgggaaa ggagggcaca
     7321 gggttcagaa gtaactactt tgcttatgaa actggaggca aggcctgcaa aatgcaatac
     7381 tgcaagcatt ggggagtcag actcccatca ggtgtctggt tcgagatggc tgataaggat
     7441 ctctttgctg cagccagatt ccctgaatgc ccagaagggt caagtatctc tgctccatct
     7501 cagacctcag tggatgtaag tctaattcag gacgttgaga ggatcttgga ttattccctc
     7561 tgccaagaaa cctggagcaa aatcagagcg ggtcttccaa tctctccagt ggatctcagc
     7621 tatcttgctc ctaaaaaccc aggaaccggt cctgctttca ccataatcaa tggtacccta
     7681 aaatactttg agaccagata catcagagtc gatattgctg ctccaatcct ctcaagaatg
     7741 gtcggaatga tcagtggaac taccacagaa agggaactgt gggatgactg ggcaccatat
     7801 gaagacgtgg aaattggacc caatggagtt ctgaggacca gttcaggata taagtttcct
     7861 ttatacatga ttggacatgg tatgttggac tccgatcttc atcttagctc aaaggctcag
     7921 gtgttcgaac atcctcacat tcaagacgct gcttcgcaac ttcctgatga tgagagttta
     7981 ttttttggtg atactgggct atccaaaaat ccaatcgagc ttgtagaagg ttggttcagt
     8041 agttggaaaa gctctattgc ctcttttttc tttatcatag ggttaatcat tggactattc
     8101 ttggttctcc gagttggtat ccatctttgc attaaattaa agcacaccaa gaaaagacag
     8161 atttatacag acatagagat gaaccgactt ggaaagtgat aaggccaggc cggccaagct
     8221 tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
     8281 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     8341 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8461 atcttatcat gtctggatct gatcactgct tgagcctagg agatccgaac cagataagtg
     8521 aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac
     8581 ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc
     8641 cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt
     8701 tttaatcaaa catcctgcca actccatgtg acaaaccgtc atcttcggct acttt
//
