LOCUS       sequence-433381-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four copies pyrrolysyl tRNA variant ""PyOtR"" with U6 
            promoter; can be packaged in bacmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Malley K, Pham Q,
            Loynd C, Huang Z, van Opijnen T, Chatterjee A
  TITLE     Enhanced Directed Evolution in Mammalian Cells Yields a 
            Hyperefficient Pyrrolysyl tRNA for Noncanonical Amino Acid 
            Mutagenesis.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jan 26:e202316428. doi: 
            10.1002/anie.202316428.
  PUBMED    38279536
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jan 26:e202316428. doi: 10.1002/anie.202316428."
COMMENT     Sequence Label: pAcBac1-BacMam-4x-PyOtR
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4082..4306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4295..4312
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4361..4949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4467..4478
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4876..4896
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(5041..5060)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5042..5060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5105..5308)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5134..5154)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5309..5688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5783..5874
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5799..5816
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(5933..5980,5981..7468)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    7614..7748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7651..7670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7705..7724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7845..8093)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(8192..8440)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(8539..8787)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(8886..9134)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     mobile_element  9165..9330
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc gcttaatgcg
     4021 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     4081 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     4141 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     4201 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     4261 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     4321 cgggtttaaa cgggccctct agactcgagt taaagtcgac gcggggaggc ggcccaaagg
     4381 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     4441 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     4501 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     4561 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     4621 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     4681 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     4741 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     4801 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     4861 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     4921 tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgttgaatt ccaccacact
     4981 ggactagtgg atccgagctc ggtaccaagc ttaagtttaa acgctagcca gcttgggtct
     5041 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
     5101 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     5161 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     5221 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     5281 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     5341 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     5401 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5461 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5521 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5581 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5641 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
     5701 tggcccgtac atcgcgaagc agcgcaaaac ggatcctgca ggtatttgcg gccgcggtcc
     5761 gtatactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     5821 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     5881 gattattcat accgtcccac catcgggcgc gaactcctaa aaaaccgcca ccatgaagtg
     5941 ccttttgtac ttagcctttt tattcattgg ggtgaattgc aagttcacca tagtttttcc
     6001 acacaaccaa aaaggaaact ggaaaaatgt tccttctaat taccattatt gcccgtcaag
     6061 ctcagattta aattggcata atgacttaat aggcacagcc ttacaagtca aaatgcccaa
     6121 gagtcacaag gctattcaag cagacggttg gatgtgtcat gcttccaaat gggtcactac
     6181 ttgtgatttc cgctggtatg gaccgaagta tataacacat tccatccgat ccttcactcc
     6241 atctgtagaa caatgcaagg aaagcattga acaaacgaaa caaggaactt ggctgaatcc
     6301 aggcttccct cctcaaagtt gtggatatgc aactgtgacg gatgccgaag cagtgattgt
     6361 ccaggtgact cctcaccatg tgctggttga tgaatacaca ggagaatggg ttgattcaca
     6421 gttcatcaac ggaaaatgca gcaattacat atgccccact gtccataact ctacaacctg
     6481 gcattctgac tataaggtca aagggctatg tgattctaac ctcatttcca tggacatcac
     6541 cttcttctca gaggacggag agctatcatc cctgggaaag gagggcacag ggttcagaag
     6601 taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa atgcaatact gcaagcattg
     6661 gggagtcaga ctcccatcag gtgtctggtt cgagatggct gataaggatc tctttgctgc
     6721 agccagattc cctgaatgcc cagaagggtc aagtatctct gctccatctc agacctcagt
     6781 ggatgtaagt ctaattcagg acgttgagag gatcttggat tattccctct gccaagaaac
     6841 ctggagcaaa atcagagcgg gtcttccaat ctctccagtg gatctcagct atcttgctcc
     6901 taaaaaccca ggaaccggtc ctgctttcac cataatcaat ggtaccctaa aatactttga
     6961 gaccagatac atcagagtcg atattgctgc tccaatcctc tcaagaatgg tcggaatgat
     7021 cagtggaact accacagaaa gggaactgtg ggatgactgg gcaccatatg aagacgtgga
     7081 aattggaccc aatggagttc tgaggaccag ttcaggatat aagtttcctt tatacatgat
     7141 tggacatggt atgttggact ccgatcttca tcttagctca aaggctcagg tgttcgaaca
     7201 tcctcacatt caagacgctg cttcgcaact tcctgatgat gagagtttat tttttggtga
     7261 tactgggcta tccaaaaatc caatcgagct tgtagaaggt tggttcagta gttggaaaag
     7321 ctctattgcc tcttttttct ttatcatagg gttaatcatt ggactattct tggttctccg
     7381 agttggtatc catctttgca ttaaattaaa gcacaccaag aaaagacaga tttatacaga
     7441 catagagatg aaccgacttg gaaagtgata aggccaggcc ggccaagctt gtcgagaagt
     7501 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7561 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7741 tctggatctg atcactgctt gagcctagca aaaaacgggc agcccgggaa tctaacccgg
     7801 ctgaacggat ttagagtccg ttcgatctac atgatcagcc gcccggtgtt tcgtcctttc
     7861 cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag
     7921 tccattttaa aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt
     7981 cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta
     8041 acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcttcctgc
     8101 ccgaggtacc cctagcaaaa aacgggcagc ccgggaatct aacccggctg aacggattta
     8161 gagtccgttc gatctacatg atcagccgcc cggtgtttcg tcctttccac aagatatata
     8221 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     8281 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     8341 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
     8401 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg aggtacccct
     8461 agcaaaaaac gggcagcccg ggaatctaac ccggctgaac ggatttagag tccgttcgat
     8521 ctacatgatc agccgcccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc
     8581 gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac
     8641 tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt
     8701 gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata
     8761 tgaaggaatc atgggaaata ggccctcttc ctgcccgagg tacccctagc aaaaaacggg
     8821 cagcccggga atctaacccg gctgaacgga tttagagtcc gttcgatcta catgatcagc
     8881 cgcccggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
     8941 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
     9001 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
     9061 ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg
     9121 ggaaataggc cctcttcctg cccgaggtac ccctaggaga tccgaaccag ataagtgaaa
     9181 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     9241 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     9301 cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt
     9361 aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact tt
//
