LOCUS       sequence-43339-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Madisen L, Mao T, Koch H, Zhuo JM, Berenyi A, Fujisawa S, Hsu YW, 
            Garcia AJ 3rd, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, 
            Boyden ES, Buzsaki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner
            EE, Zeng H
  TITLE     A toolbox of Cre-dependent optogenetic transgenic mice for 
            light-induced activation and silencing.
  JOURNAL   Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078.
  PUBMED    22446880
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2012-03-25- 25"; volume: "15"; 
            issue: "5"; pages: "793-802"
COMMENT     Sequence Label: Ai27
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(542..575)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     682..701
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aggttgctca ggcggataag gatgacagga cnagtgagca gccactctgc atagcgcagc
       61 cactgcaccc ggtgtcctgt ggcaaggtag agcatagagg gattcttaaa ctcaaaaaag
      121 aactcgagaa tcaccttaac catttcaatg gcgcacacat agatctcctc ccagccgcat
      181 gtagatttcc aggtttggta ggcatagaac atcagcagca aaatgctgaa tcctgctgca
      241 agccactgca ggncatttga cgcggtctga gcgccgttcg tgccgcgaga ttcaatccat
      301 ccggcacagt aacattgatc ctcagggacc aggacggacc cgttcaccac cacaggatta
      361 gtaacgaaca aaagttcgcg tccgacggca gacaaagcgc cgccatagtc catggtggcg
      421 ctagatcgaa ttaaacaatc cagggataag ctagcctgga ctagtaccgc ttacgcgtta
      481 agtgcaacac gtgaaggccg gccgaattcg atctagcttg ggctgcaggt cgagggacct
      541 aataacttcg tatagcatac attatacgaa gttatattaa gggttccgga tcagcttgat
      601 ggggatccag acatgataag atacattgat gagtttggac taaccacaac tagaatgcag
      661 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattat
//
