LOCUS       sequence-433426-        7559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DV3 prM-E structural cassette in pVRC8400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Chao LH, Jang J, Johnson A, Nguyen A, Gray NS, Yang PL, Harrison SC
  TITLE     How small-molecule inhibitors of dengue-virus infection interfere 
            with viral membrane fusion.
  JOURNAL   Elife. 2018 Jul 12;7. pii: 36461. doi: 10.7554/eLife.36461.
  PUBMED    29999491
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-12"; volume: "7. pii: 36461. doi"; pages: " 
            10.7554/eLife.36461"
COMMENT     Sequence Label: DV3 VLPs pVRC8400
FEATURES             Location/Qualifiers
     source          1..7559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3111..3555
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     protein_bind    complement(3582..3602)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3614..4213
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3614..3633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4110..4130
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4227..4451
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4818..4835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4989..5577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5069..5088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6119..6934)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6119..6934)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6842..6861
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6926..6945
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     7212..7230
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7268..7290)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7390..7409
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctcca tcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
      721 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
      781 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
      841 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
      901 gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc gcgccaccag
      961 acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct gcagtcaccg
     1021 tcgtcgacca ccatggatgc aatgaagaga gggctctgct gtgtgctgct gctgtgtgga
     1081 gcagtcttcg tttcgcccag cgctagcttt cacctgacca gtagggacgg cgagccaaga
     1141 atgattgtgg gcaaaaatga gaggggcaaa agcctgctgt ttaagaccgc atccgggatt
     1201 aacatgtgca ccctgatcgc catggacctg ggagagatgt gcgacgatac cgtcacatac
     1261 aagtgtcccc acattgctga ggtggaacct gaggacatcg attgctggtg taacctgact
     1321 tcaacctggg tgacctatgg aacatgtaat caggcaggag agcaccgaag agacaagcga
     1381 agcgtcgccc tggctccaca tgtgggaatg gggctggata caagaactca gacctggatg
     1441 tccgcagagg gagcttggcg acaggtcgaa aaggtggaga cctgggctct gcgacaccca
     1501 ggcttcacaa ttctggctct gtttctggca cattacatcg ggactagcct gacccagaaa
     1561 gtggtcatct tcattctgct gatcctggtg acaccctcca tggccatgag atgcgtcgga
     1621 gtgggcaaca gggactttgt cgaagggctg tccggagcaa cttgggtgga tgtggtcctg
     1681 gagcacggcg ggtgtgtgac cacaatggcc aagaacaagc ctacactgga cattgaactg
     1741 cagaagacag aggccactca gctggctact ctgaggaagc tgtgcatcga aggaaaaatt
     1801 accaatatca ctaccgatag ccgctgtcca acacagggcg aggctattct gcccgaggaa
     1861 caggaccaga actacgtctg caagcacact tatgtggata gggggtgggg aaatggctgt
     1921 gggctgttcg gaaagggctc cctggtgacc tgcgccaaat ttcagtgtct ggaatctatc
     1981 gagggcaagg tggtccagca tgagaacctg aaatacacag tcatcattac cgtgcacaca
     2041 ggggatcagc atcaggtcgg caatgaaact cagggggtga ccgccgagat tacaccccag
     2101 gcttctaccg tggaagcaat cctgcctgag tatggcactc tggggctgga atgcagtcct
     2161 cgaaccggcc tggacttcaa cgagatgatc ctgctgacca tgaagaataa ggcatggatg
     2221 gtgcaccggc agtggttctt tgatctgcca ctgccatgga ccagcggagc aacaactgaa
     2281 acaccaactt ggaaccgcaa ggagctgctg gtgaccttca agaatgcaca cgccaagaaa
     2341 caggaagtgg tcgtgctggg atcccaggag ggcgcaatgc atactgccct gaccggggct
     2401 acagaaattc agaactctgg aggcacaagt atcttcgctg gacatctgaa gtgccgcctg
     2461 aagatggaca aactggagct gaaaggcatg agttacgcaa tgtgtctgaa tacctttgtc
     2521 ctgaagaaag aagtgtcaga gactcagcac ggaaccatcc tgattaaggt ggaatataaa
     2581 ggcgaggacg ccccttgcaa gatcccattc tctaccgagg atgggcaggg aaaagcccat
     2641 aacggccggc tgattacagc taatcctgtc gtgactaaga aagaggaacc agtgaacatc
     2701 gaagctgagc ccccttttgg cgaaagcaat atcgtgattg gcatcgggga taaggcactg
     2761 aaaattaact ggtacaagaa agggagctcc atcggaaaga tgttcgaggc tactgcaaga
     2821 ggcgcaaggc gcatggccat tctgggggac accgcctggg attttggatc agtcggggga
     2881 gtgctgaata gcctgggcaa aatggtgcac cagatcttcg ggtctgcata taccgccctg
     2941 tttagcgggg tgtcctggat tatgaagatt ggaatcggcg tgctgctgac atggatcgga
     3001 ctgaacagta agaacacttc tatgagtttc tcatgtatcg ccattggaat cattactctg
     3061 tatctggggg ctgtggtgca ggctgacatg ggatgcgtct aagcggccgc agcaggtttc
     3121 cccaatgaca caaaacgtgc aacttgaaac tccgcctggt ctttacaggt ctagaggggt
     3181 aacactttgt actgcgtttg gctccacgct cgatccactg gcgagtgtta gtaacagcac
     3241 tgttgcttcg tagcggagca tgacggccgt gggaactcct ccttggtaac aaggacccac
     3301 ggggccaaaa gccacgccca cacgggcccg tcatgtgtgc aaccccagca cggcgacttt
     3361 actgcgaaac ccactttaaa gtgacattga aactggtacc cacacactgg tgacaggcta
     3421 aggatgccct tcaggtaccc cgaggtaaca cgcgacactc gggatctgag aaggggactg
     3481 gggcttctat aaaagcgctc ggtttaaaaa gcttctatgc ctgaataggt gaccggaggt
     3541 cggcaccttt cctttgcaat tactgaccct atgatacact gcaactttat tatacaaagt
     3601 tggaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3721 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3781 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3841 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3901 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3961 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4021 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4081 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4141 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4201 gcccggtgcc tgaggatcca gatctgctgt gccttctagt tgccagccat ctgttgtttg
     4261 cccctccccc gtgccttcct tgactctgga aggtgccact cccactgtcc tttcctaata
     4321 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4381 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4441 gggctctatg ggtacccagg tgctgaagaa ttgacccggt tcctcctggg ccagaaagaa
     4501 gcaggcacat ccccttctct gtgacacacc ctgtccacgc ccctggttct tagttccagc
     4561 cccactcata ggacactcat agctcaggag ggctccgcct tcaatcccac ccgctaaagt
     4621 acttggagcg gtctctccct ccctcatcag cccaccaaac caaacctagc ctccaagagt
     4681 gggaagaaat taaagcaaga taggctatta agtgcagagg gagagaaaat gcctccaaca
     4741 tgtgaggaag taatgagaga aatcatagaa ttttaaggcc atgatttaag gccatcatgg
     4801 ccttaatctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5401 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5821 gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
     5881 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
     5941 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
     6001 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
     6061 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     6121 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     6181 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     6241 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     6301 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     6361 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
     6421 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     6481 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     6541 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     6601 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     6661 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     6721 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     6781 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     6841 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     6901 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     6961 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     7021 gattttgaga cacaacgtgg ctttcccccc ccccccatta ttgaagcatt tatcagggtt
     7081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7141 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     7201 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     7261 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     7321 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     7381 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     7441 cgcacagatg cgtaaggaga aaataccgca tcagattggc tattggccat tgcatacgtt
     7501 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgtt
//
