LOCUS       sequence-433428-        7550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DV4 prM-E structural cassette in pVRC8400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Chao LH, Jang J, Johnson A, Nguyen A, Gray NS, Yang PL, Harrison SC
  TITLE     How small-molecule inhibitors of dengue-virus infection interfere 
            with viral membrane fusion.
  JOURNAL   Elife. 2018 Jul 12;7. pii: 36461. doi: 10.7554/eLife.36461.
  PUBMED    29999491
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-12"; volume: "7. pii: 36461. doi"; pages: " 
            10.7554/eLife.36461"
COMMENT     Sequence Label: DV4 VLPs pVRC8400
FEATURES             Location/Qualifiers
     source          1..7550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3102..3546
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     protein_bind    complement(3573..3593)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3605..4204
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3605..3624)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4101..4121
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4218..4442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4809..4826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4980..5568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5060..5079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6110..6925)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6110..6925)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6833..6852
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6917..6936
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     7203..7221
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7259..7281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7381..7400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctcca tcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
      721 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
      781 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
      841 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
      901 gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc gcgccaccag
      961 acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct gcagtcaccg
     1021 tcgtcgacca ccatggatgc aatgaagaga gggctctgct gtgtgctgct gctgtgtgga
     1081 gcagtcttcg tttcgcccag cgctagcttc cacctgtcca cccgagatgg ggagccactg
     1141 atgattgtcg caaagcacga gagggggaga ccactgctgt tcaaaaccac tgagggcatt
     1201 aacaaatgca ccctgatcgc catggacctg ggggagatgt gcgaagatac agtcacttac
     1261 aagtgtcctc tgctggtgaa cacagagcca gaagacattg attgctggtg taatctgacc
     1321 tccacatggg tcatgtatgg gacttgtacc cagtctggag agcggagaag ggaaaaacga
     1381 tccgtggctc tgacccctca ctctgggatg ggactggaga cacgggcaga aacttggatg
     1441 agctccgagg gcgcctggaa gcatgctcag cgagtggaaa gctggatcct gcggaaccca
     1501 ggcttcgctc tgctggcagg gtttatggcc tacatgattg gacagactgg catccagaga
     1561 accgtcttct ttgtgctgat gatgctggtg gctcccagtt atggaatgag atgcgtcggc
     1621 gtggggaata gggacttcgt cgagggcgtg tcaggaggag catgggtcga tctggtgctg
     1681 gaacacggag gctgtgtgac cacaatggcc cagggcaagc caaccctgga ctttgagctg
     1741 acaaaaacta ccgcaaagga agtggccctg ctgagaacct actgcattga ggcctccatt
     1801 tctaacatca caactgctac caggtgtcca acacaggggg aaccctacct gaaagaggaa
     1861 caggatcagc agtatatctg ccgacgagac gtggtcgatc gaggatgggg caatgggtgt
     1921 ggactgttcg gcaagggggg agtggtcaca tgcgccaagt tcagctgttc agggaagatt
     1981 actggaaacc tggtgcagat cgagaatctg gaatacactg tggtcgtgac cgtccacaat
     2041 ggagacactc atgcagtggg caacgatacc tctaatcacg gcgtgacagc catgatcact
     2101 cctcggagcc catccgtcga ggtgaagctg cccgactatg gggagctgac tctggattgc
     2161 gaacctagat ccgggattga cttcaacgag atgatcctga tgaaaatgaa gaaaaagaca
     2221 tggctggtgc ataagcagtg gtttctggac ctgccactgc catggacagc aggagctgat
     2281 acttccgagg tgcactggaa ttataaggaa cggatggtca ccttcaaagt gccccatgcc
     2341 aagagacagg atgtcacagt gctgggcagt caggagggag ctatgcactc agcactggcc
     2401 ggagctaccg aagtggactc tggagatggc aaccacatgt tcgctggaca tctgaaatgc
     2461 aaggtgagga tggagaaact gcgcattaag ggaatgagtt acacaatgtg ttctggcaag
     2521 ttcagcatcg acaaggagat ggccgaaact cagcatggca ccacagtcgt gaaagtgaag
     2581 tatgaggggg caggagcccc ctgtaaggtc cctatcgaga ttcgcgatgt gaacaaggaa
     2641 aaggtcgtgg gacgaatcat ttctagtacc cctctggcag agaacaccaa tagcgtgaca
     2701 aacatcgagc tggaaccccc tttcggcgac tcttacatcg tcattggcgt ggggaatagt
     2761 gccctgaccc tgcactggtt caggaaagga tcaagcattg gcaagatgtt tgagtccaca
     2821 tatcggggcg ctaaaagaat ggcaatcctg ggggaaactg cttgggattt cggaagcgtg
     2881 ggcgggctgt ttacctccct ggggaaggca gtccatcagg tgttcggatc agtctacact
     2941 accatgtttg gaggcgtgag ctggatgatc aggattctga tcggctttct ggtgctgtgg
     3001 atcgggacca actcacgcaa caccagcatg gccatgactt gtattgctgt gggggggatt
     3061 actctgtttc tggggtttac tgtgcaggca taagcggccg cagcaggttt ccccaatgac
     3121 acaaaacgtg caacttgaaa ctccgcctgg tctttccagg tctagagggg taacactttg
     3181 tactgcgttt ggctccacgc tcgatccact ggcgagtgtt agtaacagca ctgttgcttc
     3241 gtagcggagc atgacggccg tgggaactcc tccttggtaa caaggaccca cggggccaaa
     3301 agccacgccc acacgggccc gtcatgtgtg caaccccagc acggcgactt tactgcgaaa
     3361 cccactttaa agtgacattg aaactggtac ccacacactg gtgacaggct aaggatgccc
     3421 ttcaggtacc ccgaggtaac acgcgacact cgggatctga gaaggggact ggggcttcta
     3481 taaaagcgct cggtttaaaa agcttctatg cctgaatagg tgaccggagg tcggcacctt
     3541 tcctttgcaa ttactgaccc tatgatacac tgcaacttta ttatacaaag ttggaaagct
     3601 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3661 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3721 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3781 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     3841 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3901 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3961 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4021 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4081 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4141 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4201 ctgaggatcc agatctgctg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4261 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4321 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4381 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4441 gggtacccag gtgctgaaga attgacccgg ttcctcctgg gccagaaaga agcaggcaca
     4501 tccccttctc tgtgacacac cctgtccacg cccctggttc ttagttccag ccccactcat
     4561 aggacactca tagctcagga gggctccgcc ttcaatccca cccgctaaag tacttggagc
     4621 ggtctctccc tccctcatca gcccaccaaa ccaaacctag cctccaagag tgggaagaaa
     4681 ttaaagcaag ataggctatt aagtgcagag ggagagaaaa tgcctccaac atgtgaggaa
     4741 gtaatgagag aaatcataga attttaaggc catgatttaa ggccatcatg gccttaatct
     4801 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4861 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact cggggggggg
     5821 gggcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc
     5881 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
     5941 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     6001 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc
     6061 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     6121 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     6181 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     6241 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     6301 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     6361 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     6421 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     6481 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     6541 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     6601 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     6661 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     6721 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     6781 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     6841 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag
     6901 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     6961 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     7021 acacaacgtg gctttccccc cccccccatt attgaagcat ttatcagggt tattgtctca
     7081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7141 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7201 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     7261 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7321 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     7381 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7441 gcgtaaggag aaaataccgc atcagattgg ctattggcca ttgcatacgt tgtatccata
     7501 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
//
