LOCUS       sequence-43343-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Choe A, Phun HQ, Tieu DD, Hu YH, Carpenter EM
  TITLE     Expression patterns of Hox10 paralogous genes during lumbar spinal 
            cord development.
  JOURNAL   Gene Expr Patterns. 2006 Oct;6(7):730-7. Epub 2006 Feb 21.
  PUBMED    16495162
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr 
            Patterns."; date: "2006-10"; volume: "6(7)"; pages: "730-7. Epub 
            2006 Feb 21"
COMMENT     Sequence Label: Hoxd10 probe
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(591..607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(592..608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(636..654)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(675..691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(675..691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(688..710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    699..715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(723..729,730..747,748..753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    768..789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(906..923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatttc gggctcctgg
       61 gcgctcgcgc tttcgttcat cttcttttcc atctgcagct gggcagccga gagctgcggc
      121 ttggccgcgc cacgagggtt gagctggagc atgacagtgg agctgccttc ggggctgtta
      181 ttgtactctt gggttttccc ggtggcgtag gtctgactca gtctaaaata tccagggaca
      241 ggaacctcgg ggttctcaac aggacaagac tcggggacca gcctctggta cgaagggacc
      301 tcggcagaaa tgagtttgtt gcgcttatca gaatacatgc agcaattgga ttcttcttta
      361 atgttggcgg tgaatgagca agtggggact tgctgtgtaa caggttgctc tattcgacaa
      421 gatctgttcg ggtctgtcca actgtctact tgaggtatat aaggatgtac attcataccc
      481 atattttggt ggttcacttc tcttttggcc aaagacggga gcagtccaca ggtttgcatt
      541 ccataggtcc ccatgtctgc gctaggtggt ggcatggaaa tcaagcttat cgataccgtc
      601 gacctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
      661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      721 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      781 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      841 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcga
//
