LOCUS       sequence-433432-       13406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible shRNA non targeting control shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki 
            N, Heidenreich O, Cockerill PN, Bonifer C
  TITLE     Isoform-specific and signaling-dependent propagation of acute 
            myeloid leukemia by Wilms tumor 1.
  JOURNAL   Cell Rep. 2021 Apr 20;35(3):109010. doi: 
            10.1016/j.celrep.2021.109010.
  PUBMED    33882316
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-04-20"; volume: "35(3):109010. doi"; pages: " 
            10.1016/j.celrep.2021.109010"
COMMENT     Sequence Label: pTRIPZ-shNTC
FEATURES             Location/Qualifiers
     source          1..13406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    38..626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(91..111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(509..520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             833..1066
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1167..1184)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1173..1397
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1443..1871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1530..1549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1740..1761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1880..1900)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1885..2214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2065..2200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2127..2146
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3422..3543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3592..3608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3592..3608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3605..3627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3616..3632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3640..3646,3647..3664,3665..3670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3685..3706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3823..3840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3994..4582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4074..4093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4753..5544,5545..5613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5376..5395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5614..5718)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5766..5900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6068..6701
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6748..6873
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7370..7603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7788..7832
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8130..8247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8312..8686)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      8683..8692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8696..8714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8706..8753)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    8789..8807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8824..8842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8860..8878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8896..8914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8931..8949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8967..8985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        8997..9035
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9032..9056
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9072..9767
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           9780..9901
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9929..9943
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9971..10097
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(10192..10210)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        10223..11434
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11271..11290
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(11447..11480)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             11487..12194
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(12195..12228)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    12251..12823
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12418..12435)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12645..12664
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(12826..13406,1..22)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     13325..13345
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gacccgcaag cccggtgcct gagttcgcgt ctggaacaat caacctctgg attacaaaat
       61 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
      121 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
      181 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
      241 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
      301 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
      361 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
      421 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
      481 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
      541 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
      601 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
      661 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
      721 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
      781 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
      841 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      901 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      961 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1021 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     1081 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     1141 tgacattgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     1201 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     1261 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     1321 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     1381 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     1441 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1501 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1561 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1621 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1681 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1741 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1801 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1861 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     1921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     1981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     2161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2221 ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc
     2281 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     2341 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     2401 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     2461 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt aattcagcga
     2521 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2581 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2641 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     2701 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     2761 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     2821 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     2881 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     2941 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3001 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3061 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3121 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3181 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3241 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct
     3301 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     3361 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     3421 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3541 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     3601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     5761 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5881 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     5941 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     6001 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     6061 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     6121 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     6181 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     6241 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     6301 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     6361 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     6421 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     6481 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     6781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     6841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     6901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     6961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     7081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7201 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7261 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7321 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7381 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7441 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7501 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7561 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7621 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7681 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7741 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7801 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7861 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7921 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7981 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     8041 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     8101 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     8161 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     8221 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     8281 tggttagtac cgggcccgct ctagtccgga atcagtcctg ctcctcggcc acgaagtgca
     8341 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     8401 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     8461 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     8521 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     8581 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     8641 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtg gccctcctat
     8701 agtgagtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     8761 gcaggtccga ggttctagac gagtttactc cctatcagtg atagagaacg atgtcgagtt
     8821 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     8881 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     8941 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     9001 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcac
     9061 cggtcgccac catgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg
     9121 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     9181 gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     9241 tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc
     9301 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg
     9361 aagacggggg cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct
     9421 acaacgtcaa gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
     9481 cacgcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc
     9541 acagccagat ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca
     9601 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc
     9661 acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg
     9721 ctgtggccaa gtactgcgac ctccctagca aactggggca cagataatcg atagtttgtt
     9781 tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg
     9841 gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg
     9901 agcgatctcg cttgggcgag agtaagtagt gaagccacag atgtacttac tctcgcccaa
     9961 gcgagagtgc ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta
    10021 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
    10081 ataaattaaa tcactttttt caattggaag actaatgcgg ccggccatta ctccgtctcg
    10141 tgtcttgttg catatgtctg ctggtttgtt tgatgttgtt tgcgggcggg ccctatagtg
    10201 agtcgtatta cctaggacgc gtggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
    10261 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
    10321 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
    10381 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
    10441 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
    10501 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
    10561 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
    10621 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
    10681 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
    10741 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
    10801 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
    10861 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
    10921 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
    10981 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
    11041 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
    11101 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
    11161 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
    11221 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
    11281 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
    11341 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
    11401 taaattctgg ccgtttttgg cttttttgtt agacgctagc ggatccataa cttcgtatag
    11461 tatacattat acgaagttat gccaccatgt ctaggctgga caagagcaaa gtcataaacg
    11521 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
    11581 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
    11641 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
    11701 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
    11761 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
    11821 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
    11881 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
    11941 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
    12001 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
    12061 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
    12121 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgatttt gaccttgaca
    12181 tgctccccgg gtaaataact tcgtatagta tacattatac gaagttatgg atccgcggcc
    12241 gcaaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
    12301 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    12361 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    12421 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    12481 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    12541 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    12601 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    12661 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    12721 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
    12781 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggc caccgagtac
    12841 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
    12901 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
    12961 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    13021 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    13081 ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc ccggctggcc
    13141 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    13201 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    13261 ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg
    13321 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    13381 cccgaaggac cgcgcacctg gtgcat
//
