LOCUS       sequence-433439-        2385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  part A of TAZ-SPARK-OFF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Chung CI, Yang J, Yang X, Liu H, Ma Z, Szulzewsky F, Holland EC, 
            Shen Y, Shu X
  TITLE     Phase separation of YAP-MAML2 differentially regulates the 
            transcriptome.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 13;121(7):e2310430121. doi: 
            10.1073/pnas.2310430121. Epub 2024 Feb 5.
  PUBMED    38315854
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-13"; volume: "121(7):e2310430121. 
            doi"; pages: " 10.1073/pnas.2310430121. Epub 2024 Feb 5"
COMMENT     Sequence Label: FKBP-eGFP-TAZ
FEATURES             Location/Qualifiers
     source          1..2385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..324)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             join(367..369,370..372,373..1083)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(673..692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1020..1041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggtgtgc aggtagaaac aatctccccg ggagatggcc gcacgttccc caagagggga
       61 cagacctgtg tggtgcacta caccggtatg ctcgaagacg gcaagaagtt cgatagctcc
      121 cgagaccgaa acaagccctt caagttcatg ctgggcaagc aagaggtcat acgcggttgg
      181 gaagaaggcg tggcccagat gagcgtaggg cagcgcgcca agctgaccat tagccccgac
      241 tacgcctacg gggccaccgg gcaccccggc atcattccac cccatgcgac actcgtcttt
      301 gatgtggagc tgctcaagct ggaactcgag tccggactca gatctggcag cggtggaggc
      361 gatatcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      481 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      841 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      901 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      961 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1021 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1081 aagggatccg gcagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggc
     1141 agtgctggtg gtagtgctgg tggtagtgct ggtggctcta gaatgaatcc ggcctcggcg
     1201 ccccctccgc tcccgccgcc tgggcagcaa gtgatccacg tcacgcagga cctagacaca
     1261 gacctcgaag ccctcttcaa ctctgtcatg aatccgaagc ctagctcgtg gcggaagaag
     1321 atcctgccgg agtctttctt taaggagcct gattcgggct cgcactcgcg ccagtccagc
     1381 accgactcgt cgggcggcca cccggggcct cgactggctg ggggtgccca gcatgtccgc
     1441 tcgcactcgt cgcccgcgtc cctgcagctg ggcaccggcg cgggtgctgc gggtagcccc
     1501 gcgcagcagc acgcgcacct ccgccagcag tcctacgacg tgaccgacga gctgccactg
     1561 cccccgggct gggagatgac cttcacggcc actggccaga ggtacttcct caatcacata
     1621 gaaaaaatca ccacatggca agaccctagg aaggcgatga atcagcctct gaatcatatg
     1681 aacctccacc ctgccgtcag ttccacacca gtgcctcaga ggtccatggc agtatcccag
     1741 ccaaatctcg tgatgaatca ccaacaccag cagcagatgg cccccagtac cctgagccag
     1801 cagaaccacc ccactcagaa cccacccgca gggctcatga gtatgcccaa tgcgctgacc
     1861 actcagcagc agcagcagca gaaactgcgg cttcagagaa tccagatgga gagagaaagg
     1921 attcgaatgc gccaagagga gctcatgagg caggaagctg ccctctgtcg acagctcccc
     1981 atggaagctg agactcttgc cccagttcag gctgctgtca acccacccac gatgacccca
     2041 gacatgagat ccatcactaa taatagctca gatcctttcc tcaatggagg gccatatcat
     2101 tcgagggagc agagcactga cagtggcctg gggttagggt gctacagtgt ccccacaact
     2161 ccggaggact tcctcagcaa tgtggatgag atggatacag gagaaaacgc aggacaaaca
     2221 cccatgaaca tcaatcccca acagacccgt ttccctgatt tccttgactg tcttccagga
     2281 acaaacgttg acttaggaac tttggaatct gaagacctga tccccctctt caatgatgta
     2341 gagtctgctc tgaacaaaag tgagcccttt ctaacctggc tgtaa
//
