LOCUS       sequence-43345-e        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS190
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        752..1456
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     1470..1486
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1470..1486
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1520..1536)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1521..1537)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      2134..2381
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2150..2168)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(2394..2427)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(2485..3841)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(2485..2682)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2688..3497)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3411..3430
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3498..3841)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(3896..3929)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3965..3984)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3966..3984)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3994..4011)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3994..4010)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4003..4025)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4155..4610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4242..4261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4452..4473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4741..5544)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5545..5765)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5791..5810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5910..5932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5970..5988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6030..6533
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6570..6674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6675..6743,6744..7535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6893..6912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7706..8294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8195..8214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8448..8465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(8618..8623,8624..8641,8642..8648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8656..8672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8661..8683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8680..8696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8680..8696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8715..8735
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8717..8735
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgccgggatc gaagaaatga tggtaaatga aataggaaat caaggagcat gaaggcaaaa
       61 gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg ttgatatgat gtatttggct
      121 ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc atgctgactc tgtggcggac
      181 ccgcgctctt gccggcccgg cgataacgct gggcgtgagg ctgtgcccgg cggagttttt
      241 tgcgcctgca ttttccaagg tttaccctgc gctaaggggc gagattggag aagcaataag
      301 aatgccggtt ggggttgcga tgatgacgac cacgacaact ggtgtcatta tttaagttgc
      361 cgaaagaacc tgagtgcatt tgcaacatga gtatactaga agaatgagcc aagacttgcg
      421 agacgcgagt ttgccggtgg tgcgaacaat agagcgacca tgaccttgaa ggtgagacgc
      481 gcataaccgc tagagtactt tgaagaggaa acagcaatag ggttgctacc agtataaata
      541 gacaggtaca tacaacactg gaaatggttg tctgtttgag tacgctttca attcatttgg
      601 gtgtgcactt tattatgtta caatatggaa gggaacttta cacttctcct atgcacatat
      661 attaattaaa gtccaatgct agtagagaag gggggtaaca cccctccgcg ctcttttccg
      721 atttttttct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata
      781 tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt
      841 ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag
      901 acataatggg ctaaacaaga ctacaccaat tacactgcct cattgatggt ggtacataac
      961 gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt
     1021 tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt cttttttttt
     1081 cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa atgatggaag
     1141 acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct
     1201 gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg cacactactc
     1261 tctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaaag
     1321 tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt
     1381 cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc
     1441 aagcatacaa tcaactccaa gctggccgct ctagaactag tggatccccc gggctgcagg
     1501 aattggatat caagcttatc gataccgtcg acctcgacaa tgccagaact tggatttagc
     1561 atatcaggtg gtgtcggggg tagaggaaac ccattcagac ctgatgatga tggtatattt
     1621 gtaacaaggg tacaacctga aggaccagca tcaaaattac tgcagccagg tgataaaatt
     1681 attcaggcta atggctacag ttttataaat attgaacatg gacaagcagt gtccttgcta
     1741 aaaactttcc agaatacagt tgaactcatc attgtacgag aagttggtaa cggtgcaaaa
     1801 caagagattc gagtgagggt tgaaaaagac ggtggttctg gaggcgtttc ttctgttccg
     1861 accaatctgg aagttgttgc tgcgaccccg actagcctgc tgatcagctg ggatgcgtac
     1921 tacgactctc atgtttctta ttaccgtatc acgtacggtg aaaccggtgg taactccccg
     1981 gttcaggaat tcactgtacc tggttccaag tctactgcta ccatcagcgg cctgaaaccg
     2041 ggtgttgact ataccatcac tgtatacgca cattacaact accattacta ctctagccca
     2101 atctcgatta actaccgtac ctctagactc gagtcatgta attagttatg tcacgcttac
     2161 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     2221 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     2281 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     2341 tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac ctaataactt
     2401 cgtatagcat acattatacg aagttatatt aagggttctc gacgttttcg acactggatg
     2461 gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca
     2521 tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa
     2581 aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac
     2641 aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtactgatta gaaaaactca
     2701 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     2761 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2821 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2881 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2941 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     3001 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     3061 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     3121 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     3181 tggaatgctg ttttgccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     3241 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     3301 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     3361 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     3421 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgaaacgtga
     3481 gtcttttcct tacccatggt tgtttatgtt cggatgtgat gtgagaactg tatcctagca
     3541 agattttaaa aggaagtata tgaaagaaga acctcagtgg caaatcctaa ccttttatat
     3601 ttctctacag gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga gcgtttccct
     3661 gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg cgccgtgcgg ccatcaaaat
     3721 gtatggatgc aaatgattat acatggggat gtatgggcta aatgtacggg cgacagtcac
     3781 atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag ggtattctgg gcctccatgt
     3841 cgctggccgg gtgacccggc ggggacgagg caagctaaac agatctctag acctaataac
     3901 ttcgtatagc atacattata cgaagttata ttaagggttg tcttaattaa gggtacccaa
     3961 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     4021 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4081 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4141 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4201 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4261 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4321 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4381 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4441 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4501 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4561 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg
     4621 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa
     4681 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     4741 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     4801 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     4861 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     4921 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     4981 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     5041 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     5101 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     5161 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     5221 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     5281 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     5341 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     5401 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     5461 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     5521 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     5581 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     5641 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     5701 aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     5761 ttgaaaagct gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5821 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5881 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5941 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6001 tgtcatgata ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg
     6061 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt
     6121 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa
     6181 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga
     6241 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac
     6301 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg
     6361 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac
     6421 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
     6481 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg
     6541 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8581 ttaatgtgag ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8641 ctatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8701 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ct
//
