LOCUS       sequence-43357-e       10927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS244
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        748..1452
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     1466..1482
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1466..1482
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1516..1532)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1517..1533)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      4305..4552
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4321..4339)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(4565..4598)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(4656..6012)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(4656..4853)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(4859..5668)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5582..5601
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(5669..6012)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(6067..6100)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6136..6155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6137..6155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6165..6182)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6165..6181)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6174..6196)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6326..6781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6413..6432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6623..6644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6912..7715)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7716..7936)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7962..7981)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8081..8103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8141..8159)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8201..8704
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8741..8845
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8846..8914,8915..9706)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9064..9083)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9877..10465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10366..10385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10619..10636
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(10789..10794,10795..10812,10813..10819)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10827..10843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10832..10854
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10851..10867
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10851..10867
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10886..10906
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10888..10906
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gggatcgaag aaatgatggt aaatgaaata ggaaatcaag gagcatgaag gcaaaagaca
       61 aatataaggg tcgaacgaaa aataaagtga aaagtgttga tatgatgtat ttggctttgc
      121 ggcgccgaaa aaacgagttt acgcaattgc acaatcatgc tgactctgtg gcggacccgc
      181 gctcttgccg gcccggcgat aacgctgggc gtgaggctgt gcccggcgga gttttttgcg
      241 cctgcatttt ccaaggttta ccctgcgcta aggggcgaga ttggagaagc aataagaatg
      301 ccggttgggg ttgcgatgat gacgaccacg acaactggtg tcattattta agttgccgaa
      361 agaacctgag tgcatttgca acatgagtat actagaagaa tgagccaaga cttgcgagac
      421 gcgagtttgc cggtggtgcg aacaatagag cgaccatgac cttgaaggtg agacgcgcat
      481 aaccgctaga gtactttgaa gaggaaacag caatagggtt gctaccagta taaatagaca
      541 ggtacataca acactggaaa tggttgtctg tttgagtacg ctttcaattc atttgggtgt
      601 gcactttatt atgttacaat atggaaggga actttacact tctcctatgc acatatatta
      661 attaaagtcc aatgctagta gagaaggggg gtaacacccc tccgcgctct tttccgattt
      721 ttttctaaac cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga
      781 cttcctcttt tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc
      841 tccctaacat gtaggtggcg gaggggagat atacaataga acagatacca gacaagacat
      901 aatgggctaa acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac
      961 taatactgta gccctagact tgatagccat catcatatcg aagtttcact accctttttc
     1021 catttgccat ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt
     1081 tctctctccc ccgttgttgt ctcaccatat ccgcaatgac aaaaaaatga tggaagacac
     1141 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
     1201 aggggtatct cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
     1261 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg
     1321 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
     1381 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
     1441 atacaatcaa ctccaagctg gccgctctag aactagtgga tcccccgggc tgcaggaatt
     1501 ggatatcaag cttatcgata ccgtcgacct cgacaatgcc agaacttgga tttagcatat
     1561 caggtggtgt cgggggtaga ggaaacccat tcagacctga tgatgatggt atatttgtaa
     1621 caagggtaca acctgaagga ccagcatcaa aattactgca gccaggtgat aaaattattc
     1681 aggctaatgg ctacagtttt ataaatattg aacatggaca agcagtgtcc ttgctaaaaa
     1741 ctttccagaa tacagttgaa ctcatcattg tacgagaagt tggtaacggt gcaaaacaag
     1801 agattcgagt gagggttgaa aaagacggtg gttctggagg cgtttcttct gttccgacca
     1861 atctggaagt tgttgctgcg accccgacta gcctgctgat cagctgggat gcgtactacg
     1921 actctcatgt ttcttattac cgtatcacgt acggtgaaac cggtggtaac tccccggttc
     1981 aggaattcac tgtacctggt tccaagtcta ctgctaccat cagcggcctg aaaccgggtg
     2041 ttgactatac catcactgta tacgcacatt acaactacca ttactactct agcccaatct
     2101 cgattaacta ccgtacctct agactcgaag gagggagtgg tggaatggaa cagacacaaa
     2161 cagcagaggg cactgactta ctaattggtg acgaaaagac caacgattta ccttttgtgc
     2221 agttatttct ggaggaaata ggatgcactc aatacctgga tagctttatt cagtgcaacc
     2281 ttgtcacaga agaagaaatt aagtatctcg acaaggatat cctcattgct ttgggtgtaa
     2341 acaaaatagg agacagactc aaaattttaa ggaagtcaaa atcgttccag agagataaac
     2401 ggattgaaca ggtgaataga ttgaaaaacc tgatggaaaa agtaagctct ctatccactg
     2461 ctacgctatc gatgaattca gaattgattc ctgaaaagca ctgtgttata tttatcttaa
     2521 acgatggttc cgctaagaaa gttaatgtaa atggttgctt taatgcagat tctattaaga
     2581 aaaggctaat cagaagattg ccacatgaat tattagccac aaactccaat ggagaagtaa
     2641 ctaaaatggt ccaagattat gatgtgtttg tcttagatta taccaagaac gtactgcatt
     2701 tgctatatga cgtggaatta gtcactattt gccacgcaaa tgatcgtgtt gagaaaaata
     2761 ggctaatttt tgtttccaaa gaccaaacac caagtgataa agctatatcc acatccaaaa
     2821 aactatatct aagaacgttg agtgcattga gccaggttgg gccatcctcg tcaaatttgt
     2881 tggcacagaa caaggggatt tcgcataaca atgctgaagg gaaactccgg atcgacaaca
     2941 cagaaaagga cagaattaga cagattttta atcagaggcc tcctagcgaa tttatttcta
     3001 ccaatttggc cggatatttt cctcatacag acatgaagcg gttgcaaaag acgatgagag
     3061 agtcatttcg ccattcagca aggctaagca ttgctcaaag aagaccttta agtgcagaat
     3121 caaataatat cggtgacata ctattgaaac actcaaacgc tgttgatatg gccctattac
     3181 aaggattaga tcagacaaga ttaagcagta aacttgacac aactaaaatt ccgaagcttg
     3241 cccataaaag gccagaagat aatgatgcca tatctaacca gttagaacta ttaagtgtag
     3301 agtctggtga agaagaagat cacgatttct ttggggagga cagtgacatt gtttcattac
     3361 cgacgaaaat tgccacgccc aagaattggt taaaaggtgc ttgcattgga tcaggcagtt
     3421 ttgggagtgt ttacttgggc atgaatgctc acactggtga actaatggca gtaaagcaag
     3481 tggagataaa aaataataac attggtgttc ccacagacaa caataaacaa gccaattctg
     3541 atgagaataa tgagcaggag gaacaacaag agaaaataga agatgttggg gcggtaagtc
     3601 atccaaaaac caatcaaaat attcacagaa agatggttga tgctttacag catgaaatga
     3661 atttattgaa ggagttacat catgagaaca ttgttactta ttatggtgct tctcaagaag
     3721 gcggaaattt aaatattttt cttgaatacg ttcctggggg ttcggtttcc tccatgctga
     3781 ataattacgg tccatttgag gaatcactga ttactaattt cactaggcaa atactgattg
     3841 gggttgcgta tttgcataag aagaacatta ttcacagaga tatcaagggt gcaaatattt
     3901 tgattgatat caaaggttgc gtaaaaatta ctgattttgg tatttcaaaa aaattatcac
     3961 ctttgaataa aaaacaaaat aagagagctt ctttgcaagg ttccgtattc tggatgtcac
     4021 cagaggtggt caaacagacc gctactactg ctaaagcgga tatatggtct acaggatgtg
     4081 ttgtcattga aatgtttacc ggtaagcatc ctttcccaga tttttctcaa atgcaagcga
     4141 tcttcaaaat aggcacaaac acgacccccg agataccttc ctgggctacg tcagaaggaa
     4201 agaatttctt aagaaaggca tttgagttgg attatcaata caggcctagt gcccttgaat
     4261 tgctgcagca tccatggctg gatgcacaca taatttgact cgagtcatgt aattagttat
     4321 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag
     4381 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
     4441 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
     4501 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta
     4561 cctaataact tcgtatagca tacattatac gaagttatat taagggttct cgacgttttc
     4621 gacactggat ggcggcgtta gtatcgaatc gacagcagta tagcgaccag cattcacata
     4681 cgattgacgc atgatattac tttctgcgca cttaacttcg catctgggca gatgatgtcg
     4741 aggcgaaaaa aaatataaat cacgctaaca tttgattaaa atagaacaac tacaatataa
     4801 aaaaactata caaatgacaa gttcttgaaa acaagaatct ttttattgtc agtactgatt
     4861 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     4921 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     4981 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     5041 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     5101 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
     5161 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     5221 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     5281 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     5341 cttctaatac ctggaatgct gttttgccgg ggatcgcagt ggtgagtaac catgcatcat
     5401 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     5461 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     5521 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     5581 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     5641 tcgaaacgtg agtcttttcc ttacccatgg ttgtttatgt tcggatgtga tgtgagaact
     5701 gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta
     5761 accttttata tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg
     5821 agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc gcgccgtgcg
     5881 gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct aaatgtacgg
     5941 gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg
     6001 ggcctccatg tcgctggccg ggtgacccgg cggggacgag gcaagctaaa cagatctcta
     6061 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt gtcttaatta
     6121 agggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6181 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6241 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6301 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6361 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6421 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6481 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6661 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6721 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     6781 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     6841 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     6901 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     6961 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     7021 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     7081 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     7141 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     7201 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     7261 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     7321 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     7381 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     7441 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     7501 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     7561 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     7621 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     7681 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt
     7741 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     7801 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     7861 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     7921 gatgaattga attgaaaagc tgtggtatgg tgcactctca gtacaatctg ctctgatgcc
     7981 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8041 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8101 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8161 ttataggtta atgtcatgat aataatggtt tcttaggacg gatcgcttgc ctgtaactta
     8221 cacgcgcctc gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat
     8281 ttttatgttt tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat
     8341 gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata
     8401 tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa
     8461 tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc
     8521 attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta
     8581 gagtctttta catcttcgga aaacaaaaac tattttttct ttaatttctt tttttacttt
     8641 ctatttttaa tttatatatt tatattaaaa aatttaaatt ataattattt ttatagcacg
     8701 tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8761 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8821 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8881 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8941 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9001 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9061 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9121 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9181 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9241 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9301 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9361 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9421 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9481 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9541 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10021 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10561 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10621 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    10681 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10741 gcgcaacgca attaatgtga gttacctcac tcattaggca ccccaggctt tacactttat
    10801 gcttccggct cctatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    10861 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
    10921 gctcgcc
//
