LOCUS       sequence-433651-       13951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses an engineered 
            human procollagen type I alpha 1 with SBP and mEGFP between the pro 
            sequence and triple helical region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   McCaughey J, Stevenson NL, Cross S, Stephens DJ
  TITLE     ER-to-Golgi trafficking of procollagen in the absence of large 
            carriers.
  JOURNAL   J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 
            10.1083/jcb.201806035.
  PUBMED    30587510
REFERENCE   2  (bases 1 to 13951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035."
COMMENT     Sequence Label: pLVX-SBP-mGFP-COL1A1
FEATURES             Location/Qualifiers
     source          1..13951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             822..935
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             1449..1562
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     regulatory      1578..1587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1584..1586,1587..1589,1590..2300)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1629..1650)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1890..1909)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        6282..6781
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6317..6340)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6714..6733
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6802..7401
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6802..6821)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7298..7318
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7415..8003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7468..7488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7886..7897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8210..8843
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8972..8988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8972..8988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8985..9007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8996..9012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9020..9026,9027..9044,9045..9050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9065..9086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9203..9220)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9374..9962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9454..9473)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10133..10924,10925..10993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10756..10775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10994..11098)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11146..11280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11183..11202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11237..11256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11448..12081
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12128..12253
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12750..12983
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13168..13212
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13475..13590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        13648..13951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcgc tagcgctacc
      301 ggactcagat ctcgacaccg gtcgccacca tgttcagctt tgtggatctg cggctcctgc
      361 tgctgcttgc cgcaactgcg ctgctcacac atggccagga agagggacag gtggaagggc
      421 aggatgagga cattcctccc atcacttgcg tccagaatgg gctgaggtac cacgatcgcg
      481 atgtctggaa acccgaaccc tgtcggattt gcgtgtgcga taatggcaag gtgctgtgtg
      541 acgacgttat ctgcgacgaa accaagaact gtcccggagc tgaggtccca gagggcgaat
      601 gctgtccggt gtgtcccgat ggcagcgagt ctcccacaga ccaggaaacg accggagtag
      661 agggcccgaa aggggacaca ggaccacgag gacccagagg tccagccgga ccacccggtc
      721 gtgatgggat accagggcaa ccagggctgc ctggccctcc tggcccacct ggacctcctg
      781 gcccgcccgg cttgggtggc aactttgcag ccgctgcagc gatggacgaa aagaccaccg
      841 gatggagagg tgggcacgtt gtggaaggtc ttgctggcga gttggagcag ctgcgcgcca
      901 gactcgagca tcacccacaa gggcagagag agcccatgaa gacaccggtc gccaccatgt
      961 tcagctttgt ggacctgcgg ctcctgctgc tgcttgccgc aactgcgctg ctcacacatg
     1021 gccaggaaga gggacaggtg gaagggcagg atgaggacat tcctcccatc acttgcgtcc
     1081 agaatgggct gaggtaccac gatcgcgatg tctggaaacc cgaaccctgt cggatttgcg
     1141 tgtgcgataa tggcaaggtg ctgtgtgacg acgttatctg cgacgaaacc aagaactgtc
     1201 ccggagctga ggtcccagag ggcgaatgct gtccggtgtg tcccgatggc agcgagtctc
     1261 ccacagacca ggaaacgacc ggagtagagg gcccgaaagg ggacacagga ccacgaggac
     1321 ccagaggtcc agccggacca cccggtcgtg atgggatacc agggcaacca gggctgcctg
     1381 gccctcctgg cccacctgga cctcctggcc cgcccggctt gggtggcaac tttgcagccg
     1441 ctgcagcgat ggacgaaaag accaccggat ggagaggtgg gcacgttgtg gaaggtcttg
     1501 ctggcgagtt ggagcagctg cgcgccagac tcgagcatca cccacaaggc cagagagagc
     1561 ccatgaagac accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1801 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2041 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2101 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2281 gcatggacga gctgtacaag tgctgcagct gcccccagct gtcttatggc tatgatgaga
     2341 aatcaaccgg aggaatttcc gtgcctggcc ccatgggtcc ctctggtcct cgtggtctcc
     2401 ctggcccccc tggtgcacct ggtccccaag gcttccaagg tccccctggt gagcctggcg
     2461 agcctggagc ttcaggtccc atgggtcccc gaggtccccc aggtccccct ggaaagaatg
     2521 gagatgatgg ggaagctgga aaacctggtc gtcctggtga gcgtgggcct cctgggcctc
     2581 agggtgctcg aggattgccc ggaacagctg gcctccctgg aatgaaggga cacagaggtt
     2641 tcagtggttt ggatggtgcc aagggagatg ctggtcctgc tggtcctaag ggtgagcctg
     2701 gcagccctgg tgaaaatgga gctcctggtc agatgggccc ccgtggcctg cctggtgaga
     2761 gaggtcgccc tggagcccct ggccctgctg gtgctcgtgg aaatgatggt gctactggtg
     2821 ctgccgggcc ccctggtccc accggccccg ctggtcctcc tggcttccct ggtgctgttg
     2881 gtgctaaggg tgaagctggt ccccaagggc cccgaggctc tgaaggtccc cagggtgtgc
     2941 gtggtgagcc tggcccccct ggccctgctg gtgctgctgg ccctgctgga aaccctggtg
     3001 ctgatggaca gcctggtgct aaaggtgcca atggtgctcc tggtattgct ggtgctcctg
     3061 gcttccctgg tgcccgaggc ccctctggac cccagggccc cggcggccct cctggtccca
     3121 agggtaacag cggtgaacct ggtgctcctg gcagcaaagg agacactggt gctaagggag
     3181 agcctggccc tgttggtgtt caaggacccc ctggccctgc tggagaggaa ggaaagcgag
     3241 gagctcgagg tgaacccgga cccactggcc tgcccggacc ccctggcgag cgtggtggac
     3301 ctggtagccg tggtttccct ggcgcagatg gtgttgctgg tcccaagggt cccgctggtg
     3361 aacgtggttc tcctggccct gctggcccca aaggatctcc tggtgaagct ggtcgtcccg
     3421 gtgaagctgg tctgcctggt gccaagggtc tgactggaag ccctggcagc cctggtcctg
     3481 atggcaaaac tggcccccct ggtcccgccg gtcaagatgg tcgccccgga cccccaggcc
     3541 cacctggtgc ccgtggtcag gctggtgtga tgggattccc tggacctaaa ggtgctgctg
     3601 gagagcccgg caaggctgga gagcgaggtg ttcccggacc ccctggcgct gtcggtcctg
     3661 ctggcaaaga tggagaggct ggagctcagg gaccccctgg ccctgctggt cccgctggcg
     3721 agagaggtga acaaggccct gctggctccc ccggattcca gggtctccct ggtcctgctg
     3781 gtcctccagg tgaagcaggc aaacctggtg aacagggtgt tcctggagac cttggcgccc
     3841 ctggcccctc tggagcaaga ggcgagagag gtttccctgg cgagcgtggt gtgcaaggtc
     3901 cccctggtcc tgctggtccc cgaggggcca acggtgctcc cggcaacgat ggtgctaagg
     3961 gtgatgctgg tgcccctgga gctcccggta gccagggcgc ccctggcctt cagggaatgc
     4021 ctggtgaacg tggtgcagct ggtcttccag ggcctaaggg tgacagaggt gatgctggtc
     4081 ccaaaggtgc tgatggctct cctggcaaag atggcgtccg tggtctgacc ggccccattg
     4141 gtcctcctgg ccctgctggt gcccctggtg acaagggtga aagtggtccc agcggccctg
     4201 ctggtcccac tggagctcgt ggtgcccccg gagaccgtgg tgagcctggt ccccccggcc
     4261 ctgctggctt tgctggcccc cctggtgctg acggccaacc tggtgctaaa ggcgaacctg
     4321 gtgatgctgg tgctaaaggc gatgctggtc cccctggccc tgccggaccc gctggacccc
     4381 ctggccccat tggtaatgtt ggtgctcctg gagccaaagg tgctcgcggc agcgctggtc
     4441 cccctggtgc tactggtttc cctggtgctg ctggccgagt cggtcctcct ggcccctctg
     4501 gaaatgctgg accccctggc cctcctggtc ctgctggcaa agaaggcggc aaaggtcccc
     4561 gtggtgagac tggccctgct ggacgtcctg gtgaagttgg tccccctggt ccccctggcc
     4621 ctgctggcga gaaaggatcc cctggtgctg atggtcctgc tggtgctcct ggtactcccg
     4681 ggcctcaagg tattgctgga cagcgtggtg tggtcggcct gcctggtcag agaggagaga
     4741 gaggcttccc tggtcttcct ggcccctctg gtgaacctgg caaacaaggt ccctctggag
     4801 caagtggtga acgtggtccc cctggtccca tgggcccccc tggattggct ggaccccctg
     4861 gtgaatctgg acgtgagggg gctcctggtg ccgaaggttc ccctggacga gacggttctc
     4921 ctggcgccaa gggtgaccgt ggtgagaccg gccccgctgg accccctggt gctcctggtg
     4981 ctcctggtgc ccctggcccc gttggccctg ctggcaagag tggtgatcgt ggtgagactg
     5041 gtcctgctgg tcccgccggt cctgtcggcc ctgttggcgc ccgtggcccc gccggacccc
     5101 aaggcccccg tggtgacaag ggtgagacag gcgaacaggg cgacagaggc ataaagggtc
     5161 accgtggctt ctctggcctc cagggtcccc ctggccctcc tggctctcct ggtgaacaag
     5221 gtccctctgg agcctctggt cctgctggtc cccgaggtcc ccctggctct gctggtgctc
     5281 ctggcaaaga tggactcaac ggtctccctg gccccattgg gccccctggt cctcgcggtc
     5341 gcactggtga tgctggtcct gttggtcccc ccggccctcc tggacctcct ggtccccctg
     5401 gtcctcccag cgctggtttc gacttcagct tcctgcccca gccacctcaa gagaaggctc
     5461 acgatggtgg ccgctactac cgggctgatg atgccaatgt ggttcgtgac cgtgacctcg
     5521 aggtggacac caccctcaag agcctgagcc agcagatcga gaacatccgg agcccagagg
     5581 gcagccgcaa gaaccccgcc cgcacctgcc gtgacctcaa gatgtgccac tctgactgga
     5641 agagtggaga gtactggatt gaccccaacc aaggctgcaa cctggatgcc atcaaagtct
     5701 tctgcaacat ggagactggt gagacctgcg tgtaccccac tcagcccagt gtggcccaga
     5761 agaactggta catcagcaag aaccccaagg acaagaggca tgtctggttc ggcgagagca
     5821 tgaccgatgg attccagttc gagtatggcg gccagggctc cgaccctgcc gatgtggcca
     5881 tccagctgac cttcctgcgc ctgatgtcca ccgaggcctc ccagaacatc acctaccact
     5941 gcaagaacag cgtggcctac atggaccagc agactggcaa cctcaagaag gccctgctcc
     6001 tccagggctc caacgagatc gagatccgcg ccgagggcaa cagccgcttc acctacagcg
     6061 tcactgtcga tggctgcacg agtcacaccg gagcctgggg caagacagtg attgaataca
     6121 aaaccaccaa gtcctcccgc ctgcccatca tcgatgtggc ccccttggac gttggtgccc
     6181 cagaccagga attcggcttc gacgttggcc ctgtctgctt cctgtaactg cagtcgacgg
     6241 taccgcgggc ccgggatccc gcgactctag ataattctac cgggtagggg aggcgctttt
     6301 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6361 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6421 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6481 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6541 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6601 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6661 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     6721 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     6781 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6841 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6901 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6961 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7021 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7081 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7141 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7201 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7261 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7321 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7381 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg
     7441 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7501 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7561 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7621 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7681 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7741 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7801 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7861 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7921 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7981 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     8041 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8101 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8161 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     8221 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8281 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8341 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8401 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     8461 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     8521 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     8581 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     8641 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8701 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8761 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8821 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8881 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     8941 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9001 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9061 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9121 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9181 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9241 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9301 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9361 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9421 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9481 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9541 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9601 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9661 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9721 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9781 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9841 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9901 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9961 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10021 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10261 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11101 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    11161 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11221 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11281 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11341 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11401 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
    11461 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    11521 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    11581 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    11641 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    11701 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    11761 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    11821 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    11881 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11941 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    12001 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    12061 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    12121 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    12181 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    12241 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    12301 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    12361 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    12421 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    12481 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    12541 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    12601 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    12661 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    12721 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    12781 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    12841 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    12901 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    12961 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    13021 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    13081 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    13141 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    13201 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    13261 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    13321 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    13381 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    13441 attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg ggattggggg
    13501 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    13561 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    13621 agtttatcga taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    13681 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    13741 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    13801 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    13861 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    13921 catctacgta ttagtcatcg ctattaccat g
//
