LOCUS       sequence-433655-       11598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki 
            N, Heidenreich O, Cockerill PN, Bonifer C
  TITLE     Isoform-specific and signaling-dependent propagation of acute 
            myeloid leukemia by Wilms tumor 1.
  JOURNAL   Cell Rep. 2021 Apr 20;35(3):109010. doi: 
            10.1016/j.celrep.2021.109010.
  PUBMED    33882316
REFERENCE   2  (bases 1 to 11598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-04-20"; volume: "35(3):109010. doi"; pages: " 
            10.1016/j.celrep.2021.109010"
COMMENT     Sequence Label: pCW57.1 WT1 +1 ATG
FEATURES             Location/Qualifiers
     source          1..11598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1187..1198
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3074..3098)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3121..3688
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3138..3690
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3282..3299)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3509..3528
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3702..3704,3705..3707,3708..4421)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3747..3768)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4008..4027)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4355..4376
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4471..4495)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4513..4530
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4540..5050
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4969..4987
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5060..5656
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5060..5079)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5556..5576
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5657..5710
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5711..6328,6329..6334,6335..6370,6371..6373,6374..6409,
                     6410..6412,6413..6448,6449..6457)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6497..6517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6915..6926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7131..7364
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7436..7557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7473..7492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7527..7546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7597..7732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7732..7803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7804..7872,7873..8664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8022..8041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8835..9423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9324..9343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9577..9594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9711..9732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9747..9752,9753..9770,9771..9777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9785..9801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9790..9812
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9809..9825
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9809..9825
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9844..9864
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9846..9864
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9892..10118
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10119..10299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10346..10471
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10964..11197
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11382..11426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accaattcag tcgacgccac
      601 catgcagcaa atgggctccg acgtgcggga cctgaacgcg ctgctgcccg ccgtcccctc
      661 cctgggtggc ggcggagccg cacgaggagc agtgcctgag cgccttcact gtccactttt
      721 ccggccagtt cactggcaca gccggagcct gtcgctacgg gcccttcggt cctcctccgc
      781 ccagccaggc gtcatccggc caggccagga tgtttcctaa cgcgccctac ctgcccagct
      841 gcctcgagag ccagcccgct attcgcaatc agggttacag cacggtcacc ttcgacggga
      901 cgcccagcta cggtcacacg ccctcgcacc atgcggcgca gttccccaac cactcattca
      961 agcatgagga tcccatgggc cagcagggct cgctgggtga gcagcagtac tcggtgccgc
     1021 ccccggtcta tggctgccac acccccaccg acagctgcac cggcagccag gctttgctgc
     1081 tgaggacgcc ctacagcagt gacaatttat accaaatgac atcccagctt gaatgcatga
     1141 cctggaatca gatgaactta ggagccacct taaagggagt tgctgctggg agctccagct
     1201 cagtgaaatg gacagaaggg cagagcaacc acagcacagg gtacgagagc gataaccaca
     1261 caacgcccat cctctgcgga gcccaataca gaatacacac gcacggtgtc ttcagaggca
     1321 ttcaggatgt gcgacgtgtg cctggagtag ccccgactct tgtacggtcg gcatctgaga
     1381 ccagtgagaa acgccccttc atgtgtgctt acccaggctg caataagaga tattttaagc
     1441 tgtcccactt acagatgcac agcaggaagc acactggtga gaaaccatac cagtgtgact
     1501 tcaaggactg tgaacgaagg ttttctcgtt cagaccagct caaaagacac caaaggagac
     1561 atacaggtgt gaaaccattc cagtgtaaaa cttgtcagcg aaagttctcc cggtccgacc
     1621 acctgaagac ccacaccagg actcatacag gtgaaaagcc cttcagctgt cggtggccaa
     1681 gttgtcagaa aaagtttgcc cggtcagatg aattagtccg ccatcacaac atgcatcaga
     1741 gaaacatgac caaactccag ctggcgcttt gaggggtctc cctcggggac cgttcagtgt
     1801 cccaggcagc acagtgtgtg aactgctttc aagtctgact ctccactcct cctcactaaa
     1861 aaggaaactt cagttgatct tcttcatcca acttccaaga caagataccg gtgcttctgg
     1921 aaactaccag gtgtgcctgg aagagttggt ctctgccctg cctactttta gttgactcac
     1981 aggccctgga gaagcagcta acaatgtctg gttagttaaa agcccattgc catttggtgt
     2041 ggattttcta ctgtaagaag agccatagct gatcatgtcc ccctgaccct tcccttcttt
     2101 ttttatgctc gttttcgctg gggatggaat tattgtacca ttttctatca tggaatattt
     2161 ataggccagg gcatgtgtat gtgtctgcta atgtaaactt tgtcatggtt tccatttact
     2221 aacagcaaca gcaagaaata aatcagagag caaggcatcg ggggtgaatc ttgtctaaca
     2281 ttcccgaggt cagccaggct gctaacctgg aaagcaggat gtagttctgc caggcaactt
     2341 ttaaagctca tgcatttcaa gcagctgaag aaagaatcag aactaaccag tacctctgta
     2401 tagaaatcta aaagaatttt accattcagt taattcaatg tgaacactgg cacactgctc
     2461 ttaagaaact atgaagatct gagatttttt tgtgtatgtt tttgactctt ttgagtggta
     2521 atcatatgtg tctttataga tgtacatacc tccttgcaca aacggagggg aattcatttt
     2581 catcactggg agtgtcctta gtgtataaaa accatgctgg tatatggctt caagttgtaa
     2641 aaatgaaagc gactttaaaa gaaaataggg gatggtccag gatctccact gataagactg
     2701 tttttaagta acttaaggac ctttgggtct acaagtatat gtgaaaaaaa tgagacttac
     2761 tgggtgagga aatccattgt ttaaagatgg tcgtgtgtgt gtgtgtgtgt gtgtgttgtg
     2821 ttgtgttttg ttttttaagg gagggagttt attatttacc gttgcttgaa attactgtgt
     2881 aaatatatgt ctgataatga tttgctcttt gacaactaaa attaggactg tataagtact
     2941 agatgcatca ctgggtgttg atcttacaag atattgatga taacacttaa aattgtaacc
     3001 tgcatttttc actttgctct caattaaagt ctattcaaaa ggaataattc gcggccgcac
     3061 tcgagatatc tagacccagc tttcttgtac aaagtggtga ttcccccggg ctgcaggaat
     3121 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3181 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3241 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3301 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3361 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3421 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3481 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3541 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3601 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3661 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     3721 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3781 agttcagcgt gtctggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3841 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3901 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3961 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4021 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4081 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4141 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gcgaacttca
     4201 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4261 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4321 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4381 ccgccgggat cactctcggc atggacgagc tgtacaagta atgaattaat taagaattat
     4441 caagcttatc gataccgtcg agatatctag acccagcttt cttgtacaaa gtggtttagt
     4501 aatgaaccgg tccaccacca ccaccaccac taaggatccg gggttggggt tgcgcctttt
     4561 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     4621 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     4681 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     4741 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     4801 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     4861 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     4921 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     4981 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     5041 ctctccccag caattcacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5101 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5161 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5221 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5281 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5341 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5401 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5461 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5521 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5581 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5641 ccgcaagccc ggtgccgaag gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa
     5701 ccctggtcca atgtctagac tggacaagag caaagtcata aacggagctc tggaattact
     5761 caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     5821 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     5881 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     5941 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     6001 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     6061 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     6121 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     6181 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     6241 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     6301 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     6361 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     6421 tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc gatccaagat
     6481 atcgtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6541 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6601 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6661 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6721 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6781 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6841 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6901 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6961 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7021 ccgcctcccc gcctgtttcg cctcggcgtc cggactagag gtacctttaa gaccaatgac
     7081 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7141 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7201 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7261 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7321 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7381 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7441 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7501 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7561 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7681 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctttcaaat
     7741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9541 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9601 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9721 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9781 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9841 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     9901 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     9961 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
    10021 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
    10081 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
    10141 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10201 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10261 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    10321 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    10381 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    10441 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    10501 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    10561 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10621 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    10681 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    10741 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    10801 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10861 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10921 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10981 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    11041 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11101 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11161 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11221 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11281 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11341 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11401 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    11461 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    11521 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    11581 tcccaacccc gaggggac
//
