LOCUS       sequence-433658-       11392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible WT1 cDNA with exon 8 mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki 
            N, Heidenreich O, Cockerill PN, Bonifer C
  TITLE     Isoform-specific and signaling-dependent propagation of acute 
            myeloid leukemia by Wilms tumor 1.
  JOURNAL   Cell Rep. 2021 Apr 20;35(3):109010. doi: 
            10.1016/j.celrep.2021.109010.
  PUBMED    33882316
REFERENCE   2  (bases 1 to 11392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-04-20"; volume: "35(3):109010. doi"; pages: " 
            10.1016/j.celrep.2021.109010"
COMMENT     Sequence Label: pCW57.1 Ex8 mut WT1
FEATURES             Location/Qualifiers
     source          1..11392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             979..990
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2868..2892)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2915..3482
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2932..3484
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3076..3093)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3303..3322
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3496..3498,3499..3501,3502..4215)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3541..3562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3802..3821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4149..4170
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4265..4289)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4307..4324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4334..4844
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4763..4781
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4854..5450
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4854..4873)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5350..5370
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5451..5504
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5505..6122,6123..6128,6129..6164,6165..6167,6168..6203,
                     6204..6206,6207..6242,6243..6251)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6291..6311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6709..6720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6925..7158
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7230..7351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7267..7286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7321..7340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7391..7526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7526..7597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7598..7666,7667..8458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7816..7835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8629..9217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9118..9137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9371..9388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9505..9526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9541..9546,9547..9564,9565..9571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9579..9595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9584..9606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9603..9619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9603..9619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9638..9658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9640..9658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9686..9912
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9913..10093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10140..10265
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10758..10991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11176..11220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accaattcag tcgacgccac
      601 catgtttcct aacgcgccct acctgcccag ctgcctcgag agccagcccg ctattcgcaa
      661 tcagggttac agcacggtca ccttcgacgg gacgcccagc tacggtcaca cgccctcgca
      721 ccatgcggcg cagttcccca accactcatt caagcatgag gatcccatgg gccagcaggg
      781 ctcgctgggt gagcagcagt actcggtgcc gcccccggtc tatggctgcc acacccccac
      841 cgacagctgc accggcagcc aggctttgct gctgaggacg ccctacagca gtgacaattt
      901 ataccaaatg acatcccagc ttgaatgcat gacctggaat cagatgaact taggagccac
      961 cttaaaggga gttgctgctg ggagctccag ctcagtgaaa tggacagaag ggcagagcaa
     1021 ccacagcaca gggtacgaga gcgataacca cacaacgccc atcctctgcg gagcccaata
     1081 cagaatacac acgcacggtg tcttcagagg cattcaggat gtgcgacgtg tgcctggagt
     1141 agccccgact cttgtacggt cggcatctga gaccagtgag aaacgcccct tcatgtgtgc
     1201 ttacccaggc tgcaataaga gatattttaa gctgtcccac ttacagatgc acagcaggaa
     1261 gcacactggt gagaaaccat accagtgtga cttcaaggtg actgtgaacg aaggttttct
     1321 cgttcagacc agctcaaaag acaccaaagg agacatacag gtgtgaaacc attccagtgt
     1381 aaaacttgtc agcgaaagtt ctcccggtcc gaccacctga agacccacac caggactcat
     1441 acaggtgaaa agcccttcag ctgtcggtgg ccaagttgtc agaaaaagtt tgcccggtca
     1501 gatgaattag tccgccatca caacatgcat cagagaaaca tgaccaaact ccagctggcg
     1561 ctttgagggg tctccctcgg ggaccgttca gtgtcccagg cagcacagtg tgtgaactgc
     1621 tttcaagtct gactctccac tcctcctcac taaaaaggaa acttcagttg atcttcttca
     1681 tccaacttcc aagacaagat accggtgctt ctggaaacta ccaggtgtgc ctggaagagt
     1741 tggtctctgc cctgcctact tttagttgac tcacaggccc tggagaagca gctaacaatg
     1801 tctggttagt taaaagccca ttgccatttg gtgtggattt tctactgtaa gaagagccat
     1861 agctgatcat gtccccctga cccttccctt ctttttttat gctcgttttc gctggggatg
     1921 gaattattgt accattttct atcatggaat atttataggc cagggcatgt gtatgtgtct
     1981 gctaatgtaa actttgtcat ggtttccatt tactaacagc aacagcaaga aataaatcag
     2041 agagcaaggc atcgggggtg aatcttgtct aacattcccg aggtcagcca ggctgctaac
     2101 ctggaaagca ggatgtagtt ctgccaggca acttttaaag ctcatgcatt tcaagcagct
     2161 gaagaaagaa tcagaactaa ccagtacctc tgtatagaaa tctaaaagaa ttttaccatt
     2221 cagttaattc aatgtgaaca ctggcacact gctcttaaga aactatgaag atctgagatt
     2281 tttttgtgta tgtttttgac tcttttgagt ggtaatcata tgtgtcttta tagatgtaca
     2341 tacctccttg cacaaacgga ggggaattca ttttcatcac tgggagtgtc cttagtgtat
     2401 aaaaaccatg ctggtatatg gcttcaagtt gtaaaaatga aagcgacttt aaaagaaaat
     2461 aggggatggt ccaggatctc cactgataag actgttttta agtaacttaa ggacctttgg
     2521 gtctacaagt atatgtgaaa aaaatgagac ttactgggtg aggaaatcca ttgtttaaag
     2581 atggtcgtgt gtgtgtgtgt gtgtgtgtgt gttgtgttgt gttttgtttt ttaagggagg
     2641 gagtttatta tttaccgttg cttgaaatta ctgtgtaaat atatgtctga taatgatttg
     2701 ctctttgaca actaaaatta ggactgtata agtactagat gcatcactgg gtgttgatct
     2761 tacaagatat tgatgataac acttaaaatt gtaacctgca tttttcactt tgctctcaat
     2821 taaagtctat tcaaaaggta attcgcggcc gcactcgaga tatctagacc cagctttctt
     2881 gtacaaagtg gtgattcccc cgggctgcag gaattccgcc ccccccccct aacgttactg
     2941 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3001 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     3061 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     3121 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     3181 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3241 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3301 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3361 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     3421 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3481 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3541 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag
     3601 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3661 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     3721 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3781 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3841 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3901 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3961 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
     4021 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4081 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4141 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4201 gagctgtaca agtaatgaat taattaagaa ttatcaagct tatcgatacc gtcgagatat
     4261 ctagacccag ctttcttgta caaagtggtt tagtaatgaa ccggtccacc accaccacca
     4321 ccactaagga tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     4381 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     4441 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     4501 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     4561 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     4621 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     4681 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     4741 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     4801 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagcaattc accatgaccg
     4861 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4921 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4981 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5041 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5101 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5161 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5221 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5281 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5341 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5401 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc gaaggtagag
     5461 gttctctcct cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca
     5521 agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga
     5581 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
     5641 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc
     5701 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
     5761 accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
     5821 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
     5881 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
     5941 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     6001 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct
     6061 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     6121 gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     6181 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc
     6241 tccccgggta actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct
     6301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     6661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg
     6841 cgtccggact agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6901 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6961 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7021 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7081 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7141 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7201 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7321 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7381 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7441 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7501 gtagtgagga ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc
     7561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8281 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9661 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     9721 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     9781 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     9841 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     9901 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10081 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10141 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10201 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10261 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10321 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10381 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10441 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10501 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10561 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10621 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10921 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    11041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    11101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11341 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg ac
//
