LOCUS       sequence-433680-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate 
            induction of GAL promoter induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, 
            Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C,
            Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B
  TITLE     LowTempGAL: a highly responsive low temperature-inducible GAL system
            in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460.
  PUBMED    38808673
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460."
COMMENT     Sequence Label: pIRGAL43C
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2056..2073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2056..2072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2065..2087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2281..2300)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2400..2422
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2460..2478)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2546..2650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2651..2719,2720..3511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2869..2888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3682..4270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4171..4190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4424..4441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4558..4579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4594..4599,4600..4617,4618..4624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4632..4648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4637..4659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4656..4672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4656..4672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5049..5392
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      5785..5982
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             6940..9585
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 atactataac atacaataat aggtaccaaa atgaatacaa acgttccaat attcagttct
       61 ccggtcagag atttaccaag gtctttcgaa caaaaacatt tagcggttgt agatgcattt
      121 ttccaaacat accatgtcaa acctgatttt atcgctaggt ctcctggcag agtaaatctg
      181 attggtgagc atatagatta ttgcgatttt tcagttttgc cattagccat tgatgtggat
      241 atgctttgcg cagttaaaat tttagacgaa aaaaatccat ccattacctt aacaaatgcg
      301 gaccctaaat ttgctcagcg aaagtttgat ctgcctttag atggttccta catggccata
      361 gatccgtctg tgtcggaatg gtcgaattac tttaaatgcg gactacatgt ggcacattca
      421 tacttgaaaa aaattgctcc ggaaagattt aataatacac ccttagtagg tgcgcagatc
      481 ttttgccaga gcgatattcc tactggtggt ggactctcat ctgcatttac ttgcgcggca
      541 gcactagcca caattagagc caatatggga aaaaattttg atatttccaa aaaagacttg
      601 acccgcatca cagcggttgc tgagcactat gttggagtca ataatggtgg tatggatcaa
      661 gcaacgtctg tttatgggga agaagatcat gctctatacg tagagtttag gccaaaacta
      721 aaggccacac ctttcaagtt tcctcaattg aaaaatcatg aaatcagttt cgtcatcgcc
      781 aatactcttg taaagtctaa taaattcgaa actgctccta caaattacaa tttaagagta
      841 atagaggtaa cagttgctgc caacgccttg gcgaccagat acagcgtggc cttaccatct
      901 cacaaggaca attctaactc agaaagaggg aatctaagag attttatgga tgcttactac
      961 gccagatacg aaaaccaagc ccaaccatgg aatggagata tcggaactgg tattgaacgt
     1021 ttactcaaga tgctacaatt ggtagaagaa tctttctcga ggaaaaagag cggattcact
     1081 gtacatgaag cctctacggc gctaaactgt tcacgtgagg agttcactag agattacctg
     1141 acaacttttc ccgtccgctt ccaagtcttg aaactatatc aaagagctaa acacgtttac
     1201 tccgaatcct taagggtgct taaggcttta aaaatgatga caagtgccac ttttcacacg
     1261 gacgaagatt tctttacaga ttttggccga ctaatgaatg agtcccaggc ctcttgtgat
     1321 aaactttatg aatgttcgtg catagaaacc aatcaaatat gctcgattgc cctagcaaat
     1381 ggttctttcg gctcccgtct cactggcgct ggttggggcg gttgcactat ccaccttgtt
     1441 cctagtggcg ctaatgggaa cgtggaacag gtacgaaaag cactaatcga aaaattctac
     1501 aatgtaagat atccggatct cacagatgaa gaactaaaag acgcaattat agttccaaag
     1561 cctgccttgg gtacttgttt gtacgaacaa taaagtgctt ttaactaaga attattagtc
     1621 ttttctgctt attttttcat catagtttag aacactttat attaacgaat agtttatgaa
     1681 tctatttagg tttaaaaatt gatacagttt tataagttac tttttcaaag actcgtgctg
     1741 tctattgcat aatgcactgg aaggggaaaa aaaaggtgca cacgcgtggc tttttcttga
     1801 atttgcagtt tgaaaaataa ctacattaag gcctatggat tggtttgaag atattggtga
     1861 gtagagcacg cagcacgctg tatttacgta tttaatttta tatatttgtg catacactac
     1921 tagggaagac ttgaaaaaaa cctaggaaat gaaaaaacga cacaggaagt cccgtattta
     1981 ctattttttc cttccttttg atggggcagg gcggaaatag aggataggat aagcctactg
     2041 cttagctgtt taaacactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2101 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2161 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     2221 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     2281 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2341 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2401 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2461 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2521 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2581 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2641 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2701 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2761 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2821 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2881 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2941 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3001 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3061 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3121 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3181 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3241 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3301 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3361 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3421 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3481 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3541 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3601 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3661 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3721 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3781 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3841 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3901 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3961 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4021 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4081 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4141 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4201 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4261 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4321 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4381 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4441 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4501 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4561 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4621 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     4681 cgccagttta aactgtggga gattagaggg caaagtgtac aaagatacca ctttgtatac
     4741 gacgctgtct ccatgcgaca tgtgtacagg tgccatcatc atgtatggta ttccacgctg
     4801 tgttgtcggt gagaacgtta atttcaaaag taagggcgat taattaagga tccgctgaag
     4861 cttcgtacgc tgcaggtcga caacccttaa tgttcttcat tctacgcacg gtccttcaat
     4921 tcgcttggcg gagatctatg acaagagggt cgaactcgcc taagtcgtaa ttgagtcagt
     4981 tacgctaggg ataacagggt aatatagctg tttagcttgc ctcgtccccg ccgggtcacc
     5041 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
     5101 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
     5161 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
     5221 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
     5281 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
     5341 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtat
     5401 gaccgaccaa gcgacgccca acctgccatc acgagatttc gatcccaccg ccgccttcta
     5461 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5521 ggatctcatg ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag
     5581 ctgctgcctg aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat
     5641 ccaggaaacc agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg
     5701 cacgatggcc gctttggtcg acccggacgg gacgctcctg cgcctgatac agaacgaatt
     5761 gcttgcaggc atctcatgat cagtactgac aataaaaaga ttcttgtttt caagaacttg
     5821 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     5881 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     5941 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     6001 cgccgccatc cagtgtcgaa aacgagctct cgagaaccct taaagttacg ctagggataa
     6061 cagggtaata taggttcttc attctacgca cggtccttca attcgcttgg cggtctagaa
     6121 tgacaagagg gtcgaactcg cctaagtcgt aattgagtcc agatccactg cggctctgca
     6181 tatagaataa caaacaaatc gtatttttcc cgaagccaag aaaaaaagga gactataacc
     6241 tttcaagcca gacggaaaac tatctgccca aggacaaact ggcatgggct gcttcaatgt
     6301 tcggctacct tttttctctt ttaacggggt ttggtatttt ttttctttac ttcataaacg
     6361 aatgttaaac gactgtgaaa aagccatccc tggattatgc gagagtactc tcagggcacc
     6421 cgtccgttta aagaccccag gccaaaataa aaatttcttg aaggaaaaaa atacacaggg
     6481 ccttgaactg aatacaaagc catctttttt ctcgtttgtc agcacgtccc agaaaaacgc
     6541 gcagccgtag ctcttgataa gtggcttctg cgttttgcaa cgcttttctg atctcatgtt
     6601 ttcttatgct gcgggacatc attgtcgttg agaaaagttt caatttcaaa cgaactgcct
     6661 accgttgcat tctccttgga gaagggcctg agtttagggg aatcacgtct ggctattaca
     6721 tgctacatcg tgcagtagct cctacatcgc taacaggcaa atcctacacc ctctttaaat
     6781 cgtttatctt ttatatatta tctggcctaa atttttacat ataaatattt atcatattat
     6841 gacagctcaa atgaatttcc ggcaccaagt catatgagca ctcgctactg ttcgaagaac
     6901 aaaaaatata aagcttctac aaaatttcca gaattcacaa tgaagctact gtcttctatc
     6961 gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag
     7021 tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct
     7081 ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
     7141 tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag
     7201 gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc
     7261 acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata
     7321 agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg
     7381 attgactcgg cagctcatca tgataactcc acaattccgt tggattttat gcccagggat
     7441 gctcttcatg gatttgattg gtctgaagag gatgacatgt cggatggctt gcccttcctg
     7501 aaaacggacc ccaacaataa tgggttcttt ggcgacggtt ctctcttatg tattcttcga
     7561 tctattggct ttaaaccgga aaattacacg aactctaacg ttaacaggct cccgaccatg
     7621 attacggata gatacacgtt ggcttctaga tccacaacat cccgtttact tcaaagttat
     7681 ctcaataatt ttcaccccta ctgccctatc gtgcactcac cgacgctaat gatgttgtat
     7741 aataaccaga ttgaaatcgc gtcgaaggat caatggcaaa tcctttttaa ctgcatatta
     7801 gccattggag cctggtgtat agagggggaa tctactgata tagatgtttt ttactatcaa
     7861 aatgctaaat ctcatttgac gagcaaggtc ttcgagtcag gttccataat tttggtgaca
     7921 gccctacatc ttctgtcgcg atatacacag tggaggcaga aaacaaatac tagctataat
     7981 tttcacagct tttccataag aatggccata tcattgggct tgaataggga cctcccctcg
     8041 tccttcagtg atagcagcat tctggaacaa agacgccgaa tttggtggtc tgtctactct
     8101 tgggagatcc aattgtccct gctttatggt cgatccatcc agctttctca gaatacaatc
     8161 tccttccctt cttctgtcga cgatgtgcag cgtaccacaa caggtcccac catatatcat
     8221 ggcatcattg aaacagcaag gctcttacaa gttttcacaa aaatctatga actagacaaa
     8281 acagtaactg cagaaaaaag tcctatatgt gcaaaaaaat gcttgatgat ttgtaatgag
     8341 attgaggagg tttcgagaca ggcaccaaag tttttacaaa tggatatttc caccaccgct
     8401 ctaaccaatt tgttgaagga acacccttgg ctatccttta caagattcga actgaagtgg
     8461 aaacagttgt ctcttatcat ttatgtatta agagattttt tcactaattt tacccagaaa
     8521 aagtcacaac tagaacagga tcaaaatgat catcaaagtt atgaagttaa acgatgctcc
     8581 atcatgttaa gcgatgcagc acaaagaact gttatgtctg taagtagcta tatggacaat
     8641 cataatgtca ccccatattt tgcctggaat tgttcttatt acttgttcaa tgcagtccta
     8701 gtacccataa agactctact ctcaaactca aaatcgaatg ctgagaataa cgagaccgca
     8761 caattattac aacaaattaa cactgttctg atgctattaa aaaaactggc cacttttaaa
     8821 atccagactt gtgaaaaata cattcaagta ctggaagagg tatgtgcgcc gtttctgtta
     8881 tcacagtgtg caatcccatt accgcatatc agttataaca atagtaatgg tagcgccatt
     8941 aaaaatattg tcggttctgc aactatcgcc caatacccta ctcttccgga ggaaaatgtc
     9001 aacaatatca gtgttaaata tgtttctcct ggctcagtag ggccttcacc tgtgccattg
     9061 aaatcaggag caagtttcag tgatctagtc aagctgttat ctaaccgtcc accctctcgt
     9121 aactctccag tgacaatacc aagaagcaca ccttcgcatc gctcagtcac gccttttcta
     9181 gggcaacagc aacagctgca atcattagtg ccactgaccc cgtctgcttt gtttggtggc
     9241 gccaatttta atcaaagtgg gaatattgct gatagctcat tgtccttcac tttcactaac
     9301 agtagcaacg gtccgaacct cataacaact caaacaaatt ctcaagcgct ttcacaacca
     9361 attgcctcct ctaacgttca tgataacttc atgaataatg aaatcacggc tagtaaaatt
     9421 gatgatggta ataattcaaa accactgtca cctggttgga cggaccaaac tgcgtataac
     9481 gcgtttggaa tcactacagg gatgtttaat accactacaa tggatgatgt atataactat
     9541 ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agtaaaatga atcgtagata
     9601 ctgaaaaacc ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat
     9661 ttatacatcg ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca
     9721 tgtaacctct gatctataga attttttaaa tgactagaat taatgcccat cttttttttg
     9781 gacctaaatt cttcatgaaa atatattacg agggcttatt cagaagcttc gctcatataa
     9841 cgaaaaaaag ggtttggatc gaacgtaatt gagattgatt agttaatact caaaataaaa
     9901 cagctcctac caccagtgta aagtagaacg ttaatagagc caatgtcttc agacagcggc
     9961 cgcggaagtg tctcataaac tttactcaag agttaagtac tgaaaaattc gacttttatg
    10021 atagttcaag tgtcgacgct gcgggtatag aaagggttct ttactctata gtacctcctc
    10081 gctcagcatc tgcttcttcc caaagatgaa cgcggcgtta tgtcactaac gacgtgcacc
    10141 aacttgcgga aagtggaatc ccgttccaaa actggcatcc actaattgat acatctacac
    10201 accgcacgcc ttttttctga agcccacttt cgtggacttt gccatatgca aaattcatga
    10261 agtgtgatac caagtcagca tacacctcac tagggtagtt tctttggttg tattgatcat
    10321 ttggttcatc gtggttcatt aatttttttt ctccattgct ttctggcttt gatcttacta
    10381 tcatttggat ttttgtcgaa ggttgtagaa ttgtatgtga caagtggcac caagcatata
    10441 taaaaaaaaa aagcattatc ttcctaccag agttgattgt taaaaacgta tttatagcaa
    10501 acgcaattgt aattaattct tattttgtat cttttcttcc cttgtctcaa tcttttattt
    10561 ttattttatt tttcttttct tagtttcttt cataacacca agcaacta
//
