LOCUS       sequence-433681-       11431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate 
            induction of GAL promoter induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Lu Z, Shen Q, Bandari NC, Evans S, McDonnell L, Liu L, Jin W, 
            Luna-Flores CH, Collier T, Talbo G, McCubbin T, Esquirol L, Myers C,
            Trau M, Dumsday G, Speight R, Howard CB, Vickers CE, Peng B
  TITLE     LowTempGAL: a highly responsive low temperature-inducible GAL system
            in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460.
  PUBMED    38808673
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 May 29:gkae460. doi: 10.1093/nar/gkae460."
COMMENT     Sequence Label: pIRHDGAL43C
FEATURES             Location/Qualifiers
     source          1..11431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(225..242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(225..241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(234..256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(450..469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     569..591
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(629..647)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        715..819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(820..888,889..1680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1038..1057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1851..2439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2340..2359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2593..2610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2727..2748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2763..2768,2769..2786,2787..2793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2801..2817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2806..2828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2825..2841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2825..2841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3218..3561
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3954..4151
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             5109..7754
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             8808..9035
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(9613..9630)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctatggatt ggtttgaaga tattggtgag tagagcacgc agcacgctgt atttacgtat
       61 ttaattttat atatttgtgc atacactact agggaagact tgaaaaaaac ctaggaaatg
      121 aaaaaacgac acaggaagtc ccgtatttac tattttttcc ttccttttga tggggcaggg
      181 cggaaataga ggataggata agcctactgc ttagctgttt aaacactggc cgtcgtttta
      241 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      301 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      361 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      421 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      481 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      541 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      601 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      661 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      841 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      901 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      961 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1021 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1081 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1141 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1201 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1261 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1321 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1381 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1441 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1501 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1561 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1621 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1681 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1981 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2521 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2581 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2641 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2701 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2761 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2821 cacacaggaa acagctatga ccatgattac gccagtttaa actgtgggag attagagggc
     2881 aaagtgtaca aagataccac tttgtatacg acgctgtctc catgcgacat gtgtacaggt
     2941 gccatcatca tgtatggtat tccacgctgt gttgtcggtg agaacgttaa tttcaaaagt
     3001 aagggcgatt aattaaggat ccgctgaagc ttcgtacgct gcaggtcgac aacccttaat
     3061 gttcttcatt ctacgcacgg tccttcaatt cgcttggcgg agatctatga caagagggtc
     3121 gaactcgcct aagtcgtaat tgagtcagtt acgctaggga taacagggta atatagctgt
     3181 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     3241 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     3301 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     3361 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     3421 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     3481 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     3541 cacatccgaa cataaacaac catgggtatg accgaccaag cgacgcccaa cctgccatca
     3601 cgagatttcg atcccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     3661 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     3721 gggctcgatc ccctcgcgag ttggttcagc tgctgcctga ggctggacga cctcgcggag
     3781 ttctaccggc agtgcaaatc cgtcggcatc caggaaacca gcagcggcta tccgcgcatc
     3841 catgcccccg aactgcagga gtggggaggc acgatggccg ctttggtcga cccggacggg
     3901 acgctcctgc gcctgataca gaacgaattg cttgcaggca tctcatgatc agtactgaca
     3961 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     4021 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     4081 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     4141 tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctctc
     4201 gagaaccctt aaagttacgc tagggataac agggtaatat aggttcttca ttctacgcac
     4261 ggtccttcaa ttcgcttggc ggtctagaat gacaagaggg tcgaactcgc ctaagtcgta
     4321 attgagtcca gatccactgc ggctctgcat atagaataac aaacaaatcg tatttttccc
     4381 gaagccaaga aaaaaaggag actataacct ttcaagccag acggaaaact atctgcccaa
     4441 ggacaaactg gcatgggctg cttcaatgtt cggctacctt ttttctcttt taacggggtt
     4501 tggtattttt tttctttact tcataaacga atgttaaacg actgtgaaaa agccatccct
     4561 ggattatgcg agagtactct cagggcaccc gtccgtttaa agaccccagg ccaaaataaa
     4621 aatttcttga aggaaaaaaa tacacagggc cttgaactga atacaaagcc atcttttttc
     4681 tcgtttgtca gcacgtccca gaaaaacgcg cagccgtagc tcttgataag tggcttctgc
     4741 gttttgcaac gcttttctga tctcatgttt tcttatgctg cgggacatca ttgtcgttga
     4801 gaaaagtttc aatttcaaac gaactgccta ccgttgcatt ctccttggag aagggcctga
     4861 gtttagggga atcacgtctg gctattacat gctacatcgt gcagtagctc ctacatcgct
     4921 aacaggcaaa tcctacaccc tctttaaatc gtttatcttt tatatattat ctggcctaaa
     4981 tttttacata taaatattta tcatattatg acagctcaaa tgaatttccg gcaccaagtc
     5041 atatgagcac tcgctactgt tcgaagaaca aaaaatataa agcttctaca aaatttccag
     5101 aattcacaat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
     5161 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
     5221 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
     5281 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     5341 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     5401 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     5461 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     5521 acaaaggtca aagacagttg actgtatcga ttgactcggc agctcatcat gataactcca
     5581 caattccgtt ggattttatg cccagggatg ctcttcatgg atttgattgg tctgaagagg
     5641 atgacatgtc ggatggcttg cccttcctga aaacggaccc caacaataat gggttctttg
     5701 gcgacggttc tctcttatgt attcttcgat ctattggctt taaaccggaa aattacacga
     5761 actctaacgt taacaggctc ccgaccatga ttacggatag atacacgttg gcttctagat
     5821 ccacaacatc ccgtttactt caaagttatc tcaataattt tcacccctac tgccctatcg
     5881 tgcactcacc gacgctaatg atgttgtata ataaccagat tgaaatcgcg tcgaaggatc
     5941 aatggcaaat cctttttaac tgcatattag ccattggagc ctggtgtata gagggggaat
     6001 ctactgatat agatgttttt tactatcaaa atgctaaatc tcatttgacg agcaaggtct
     6061 tcgagtcagg ttccataatt ttggtgacag ccctacatct tctgtcgcga tatacacagt
     6121 ggaggcagaa aacaaatact agctataatt ttcacagctt ttccataaga atggccatat
     6181 cattgggctt gaatagggac ctcccctcgt ccttcagtga tagcagcatt ctggaacaaa
     6241 gacgccgaat ttggtggtct gtctactctt gggagatcca attgtccctg ctttatggtc
     6301 gatccatcca gctttctcag aatacaatct ccttcccttc ttctgtcgac gatgtgcagc
     6361 gtaccacaac aggtcccacc atatatcatg gcatcattga aacagcaagg ctcttacaag
     6421 ttttcacaaa aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt cctatatgtg
     6481 caaaaaaatg cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag gcaccaaagt
     6541 ttttacaaat ggatatttcc accaccgctc taaccaattt gttgaaggaa cacccttggc
     6601 tatcctttac aagattcgaa ctgaagtgga aacagttgtc tcttatcatt tatgtattaa
     6661 gagatttttt cactaatttt acccagaaaa agtcacaact agaacaggat caaaatgatc
     6721 atcaaagtta tgaagttaaa cgatgctcca tcatgttaag cgatgcagca caaagaactg
     6781 ttatgtctgt aagtagctat atggacaatc ataatgtcac cccatatttt gcctggaatt
     6841 gttcttatta cttgttcaat gcagtcctag tacccataaa gactctactc tcaaactcaa
     6901 aatcgaatgc tgagaataac gagaccgcac aattattaca acaaattaac actgttctga
     6961 tgctattaaa aaaactggcc acttttaaaa tccagacttg tgaaaaatac attcaagtac
     7021 tggaagaggt atgtgcgccg tttctgttat cacagtgtgc aatcccatta ccgcatatca
     7081 gttataacaa tagtaatggt agcgccatta aaaatattgt cggttctgca actatcgccc
     7141 aataccctac tcttccggag gaaaatgtca acaatatcag tgttaaatat gtttctcctg
     7201 gctcagtagg gccttcacct gtgccattga aatcaggagc aagtttcagt gatctagtca
     7261 agctgttatc taaccgtcca ccctctcgta actctccagt gacaatacca agaagcacac
     7321 cttcgcatcg ctcagtcacg ccttttctag ggcaacagca acagctgcaa tcattagtgc
     7381 cactgacccc gtctgctttg tttggtggcg ccaattttaa tcaaagtggg aatattgctg
     7441 atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc ataacaactc
     7501 aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca
     7561 tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac
     7621 ctggttggac ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata
     7681 ccactacaat ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaacc
     7741 caaaaaaaga gtaaaatgaa tcgtagatac tgaaaaaccc cgcaagttca cttcaactgt
     7801 gcatcgtgca ccatctcaat ttctttcatt tatacatcgt tttgccttct tttatgtaac
     7861 tatactcctc taagtttcaa tcttggccat gtaacctctg atctatagaa ttttttaaat
     7921 gactagaatt aatgcccatc ttttttttgg acctaaattc ttcatgaaaa tatattacga
     7981 gggcttattc agaagcttcg ctcatataac gaaaaaaagg gtttggatcg aacgtaattg
     8041 agattgatta gttaatactc aaaataaaac agctcctacc accagtgtaa agtagaacgt
     8101 taatagagcc aatgtcttca gacagcggcc gcggaagtgt ctcataaact ttactcaaga
     8161 gttaagtact gaaaaattcg acttttatga tagttcaagt gtcgacgctg cgggtataga
     8221 aagggttctt tactctatag tacctcctcg ctcagcatct gcttcttccc aaagatgaac
     8281 gcggcgttat gtcactaacg acgtgcacca acttgcggaa agtggaatcc cgttccaaaa
     8341 ctggcatcca ctaattgata catctacaca ccgcacgcct tttttctgaa gcccactttc
     8401 gtggactttg ccatatgcaa aattcatgaa gtgtgatacc aagtcagcat acacctcact
     8461 agggtagttt ctttggttgt attgatcatt tggttcatcg tggttcatta attttttttc
     8521 tccattgctt tctggctttg atcttactat catttggatt tttgtcgaag gttgtagaat
     8581 tgtatgtgac aagtggcacc aagcatatat aaaaaaaaaa agcattatct tcctaccaga
     8641 gttgattgtt aaaaacgtat ttatagcaaa cgcaattgta attaattctt attttgtatc
     8701 ttttcttccc ttgtctcaat cttttatttt tattttattt ttcttttctt agtttctttc
     8761 ataacaccaa gcaactaata ctataacata caataatagg taccaaaatg cagattttcg
     8821 tcaagacttt gaccggtaaa accataacat tggaagttga atcttccgat accatcgaca
     8881 acgttaagtc gaaaattcaa gacaaggaag gtatccctcc agatcaacaa agattgatct
     8941 ttgccggtaa gcagctagaa gacggtagaa cgctgtctga ttacaacatt cagaaggagt
     9001 ccaccttaca tcttgtgcta aggctaagag gtggtagaca tggttctggt atcatggtta
     9061 gaccattgaa ctgtatcgtt gctgtttctc aaaacatggg tattggaaag aatggtgact
     9121 tgccttggcc accattgaga aatgagttta agtacttcca gagaatgacc accacttctt
     9181 cagttgaagg taaacaaaac ttggtcatca tgggtagaaa gacctggttt tctattccag
     9241 aaaagaacag gttgttgaag gacagaatca acatcgtttt gtccagggaa ttgaaagaac
     9301 caccaagagg tgctcatttc ttggcaaaat ctttggatga tgccttgaga ttgatcgaac
     9361 aaccagaatt ggcttccaag gttgatatgg tttggattgt cggtggttca tctgtttatc
     9421 aagaagccat gaatcaacca ggtcatttga ggttgttcgt taccagaatc atgcaagagt
     9481 ttgagtctga tactttcttc cctgaaattg acttgggcaa gtataagttg ttgccagaat
     9541 atccaggtgt cttgtctgaa gttcaagaag aaaagggtat caagtacaag ttcgaagtct
     9601 acgaaaagaa ggatggtggt ggtggtggtg gtaatacaaa cgttccaata ttcagttctc
     9661 cggtcagaga tttaccaagg tctttcgaac aaaaacattt agcggttgta gatgcatttt
     9721 tccaaacata ccatgtcaaa cctgatttta tcgctaggtc tcctggcaga gtaaatctga
     9781 ttggtgagca tatagattat tgcgattttt cagttttgcc attagccatt gatgtggata
     9841 tgctttgcgc agttaaaatt ttagacgaaa aaaatccatc cattacctta acaaatgcgg
     9901 accctaaatt tgctcagcga aagtttgatc tgcctttaga tggttcctac atggccatag
     9961 atccgtctgt gtcggaatgg tcgaattact ttaaatgcgg actacatgtg gcacattcat
    10021 acttgaaaaa aattgctccg gaaagattta ataatacacc cttagtaggt gcgcagatct
    10081 tttgccagag cgatattcct actggtggtg gactctcatc tgcatttact tgcgcggcag
    10141 cactagccac aattagagcc aatatgggaa aaaattttga tatttccaaa aaagacttga
    10201 cccgcatcac agcggttgct gagcactatg ttggagtcaa taatggtggt atggatcaag
    10261 caacgtctgt ttatggggaa gaagatcatg ctctatacgt agagtttagg ccaaaactaa
    10321 aggccacacc tttcaagttt cctcaattga aaaatcatga aatcagtttc gtcatcgcca
    10381 atactcttgt aaagtctaat aaattcgaaa ctgctcctac aaattacaat ttaagagtaa
    10441 tagaggtaac agttgctgcc aacgccttgg cgaccagata cagcgtggcc ttaccatctc
    10501 acaaggacaa ttctaactca gaaagaggga atctaagaga ttttatggat gcttactacg
    10561 ccagatacga aaaccaagcc caaccatgga atggagatat cggaactggt attgaacgtt
    10621 tactcaagat gctacaattg gtagaagaat ctttctcgag gaaaaagagc ggattcactg
    10681 tacatgaagc ctctacggcg ctaaactgtt cacgtgagga gttcactaga gattacctga
    10741 caacttttcc cgtccgcttc caagtcttga aactatatca aagagctaaa cacgtttact
    10801 ccgaatcctt aagggtgctt aaggctttaa aaatgatgac aagtgccact tttcacacgg
    10861 acgaagattt ctttacagat tttggccgac taatgaatga gtcccaggcc tcttgtgata
    10921 aactttatga atgttcgtgc atagaaacca atcaaatatg ctcgattgcc ctagcaaatg
    10981 gttctttcgg ctcccgtctc actggcgctg gttggggcgg ttgcactatc caccttgttc
    11041 ctagtggcgc taatgggaac gtggaacagg tacgaaaagc actaatcgaa aaattctaca
    11101 atgtaagata tccggatctc acagatgaag aactaaaaga cgcaattata gttccaaagc
    11161 ctgccttggg tacttgtttg tacgaacaat aaagtgcttt taactaagaa ttattagtct
    11221 tttctgctta ttttttcatc atagtttaga acactttata ttaacgaata gtttatgaat
    11281 ctatttaggt ttaaaaattg atacagtttt ataagttact ttttcaaaga ctcgtgctgt
    11341 ctattgcata atgcactgga aggggaaaaa aaaggtgcac acgcgtggct ttttcttgaa
    11401 tttgcagttt gaaaaataac tacatgtaag g
//
