LOCUS       sequence-433689-       10641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene 
            amplification) for the expression of AeBlue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Evans S, Lu Z, McDonnell L, Anderson W, Peralta F, Watkins T , Ahmed
            H, Luna-Flores CH, Loan T, Navone L, Trau M, Scott CH, Speight R, 
            Vickers CE, Peng B
  TITLE     The integration of tandem gene repeats via a bacterial type-II 
            toxin-antitoxin-mediated gene amplification (ToxAmp) system and 
            stability visualisation in Saccharomyces cerevisiae
  JOURNAL   bioRxiv 2024.01.30.578080
REFERENCE   2  (bases 1 to 10641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.30.578080"
COMMENT     Sequence Label: pToxAmp21R
FEATURES             Location/Qualifiers
     source          1..10641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(199..232)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2523..2556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2776..3010
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     promoter        6192..6479
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     6349..6372
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(8014..8030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8014..8030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8027..8049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8038..8054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8062..8068,8069..8086,8087..8092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8107..8128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8245..8262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8416..9004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8496..8515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9175..9966,9967..10035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9798..9817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10036..10140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10208..10226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10264..10286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10386..10405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10599..10621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10613..10630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10614..10630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aacgccaaac ttattacttt tcctatttga ggttggtatt gattgttgtc aaagaatgaa
       61 aatatacaca aacgccacaa tatacgtacc aggttcacga aaactgatcg tatggttcat
      121 accctgactt ggcaaaccta atgtgaccgt cgctgattag cggatcgcat aggccactag
      181 tggatctgat atcacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg
      241 ttctcgagag ctcgctgtga agatcccagc aaaggcttac aaagtgttat ctcttttgag
      301 acttgttgag ttgaacactg gtgttttcat caaacttacc aaggacgtgt acccattgtt
      361 gaaacttgta tcaccatata ttgttatcgg acaaccttca cttgcatcta tccgttcttt
      421 aatccaaaag agatctagaa taatgtggca aaggccagaa gataaagaac caaaagagat
      481 aatcttgaat gacaacaata tcgttgaaga gaaattaggt gatgaaggtg tcatttgtat
      541 cgaggatatc atccatgaga tttcgacgtt gggcgaaaat ttctcgaaat gtactttctt
      601 cctattacca ttcaaattga acagagaagt cagtggattc ggtgccatct cccgtttgaa
      661 taaactgaaa atgcgcgaac aaaacaagaa gactcgtcaa atttcaaacg ctgccacggc
      721 tccagttatc caagtagata tcgactcaat gatttccaag ttgaattgat taactataaa
      781 aggaaaatat ctgtacaata gacatcgggc tcccattggc cctacccaca tatgtagaaa
      841 tacattactc tattcactac tgcatttagt tatgtttaac atttgatata gcagacgcta
      901 ccgccaggca caatatattc cccttccctc ttgccattcg ctgtacttgt ggtagattcc
      961 aattcagcgc agtcacgtgc tagtaatcac cgcatttttt tcttttcctt tcaggctaaa
     1021 accggttccg ggcccgatcc ctgcactcat tttctaacgg aaaaccttca gaagcataac
     1081 tacccattcc agtttagagt catgacaggt tcaacatcag atgcttcata tacttttata
     1141 tattgaatta tataaatata tctatgtact ctaagtaagt acatctgctt taacgcattc
     1201 ctacatttgc ttcgatttat ttttattgtt gatacctatt tgaagaagta aaaagtatcc
     1261 cacactacac agattatacc atgtctaaga atatcgttgt cctaccgggt gatcacgtcg
     1321 gtaaagaagt tactgacgaa gctattaagg tcttgaatgc cattgctgaa gtccgtccag
     1381 aaattaagtt caatttccaa catcacttga tcgggggtgc tgccatcgat gccactggca
     1441 ctcctttacc agatgaagct ctagaagcct ctaagaaagc cgatgctgtc ttactaggtg
     1501 ctgttggtgg tccaaaatgg ggtacgggcg cagttagacc agaacaaggt ctattgaaga
     1561 tcagaaagga attgggtcta tacgccaact tgagaccatg taactttgct tctgattctt
     1621 tactagatct ttctcctttg aagcctgaat atgcaaaggg taccgatttc gtcgtcgtta
     1681 gagaattggt tggtggtatc tactttggtg aaagaaaaga agatgaaggt gacggagttg
     1741 cttgggactc tgagaaatac agtgttcctg aagttcaaag aattacaaga atggctgctt
     1801 tcttggcatt gcaacaaaac ccaccattac caatctggtc tcttgacaag gctaacgtgc
     1861 ttgcctcttc cagattgtgg agaaagactg ttgaagaaac catcaagact gagttcccac
     1921 aattaactgt tcagcaccaa ttgatcgact ctgctgctat gattttggtt aaatcaccaa
     1981 ctaagctaaa cggtgttgtt attaccaaca acatgtttgg tgatattatc tccgatgaag
     2041 cctctgttat tccaggttct ttgggtttat taccttctgc atctctagct tccctacctg
     2101 acactaacaa ggcattcggt ttgtacgaac catgtcatgg ttctgcccca gatttaccag
     2161 caaacaaggt taacccaatt gctaccatct tatctgcagc tatgatgttg aagttatcct
     2221 tggatttggt tgaagaaggt agggctcttg aagaagctgt tagaaatgtc ttggatgcag
     2281 gtgtcagaac cggtgacctt ggtggttcta actctaccac tgaggttggc gatgctatcg
     2341 ccaaggctgt caaggaaatc ttggcttaaa gagtcttttg taacgacccc gtctccacca
     2401 acttggtatg cttgaaatct caaggccatt acacattcag ttatgtgaac gaaaggtctt
     2461 tatttaacgt agcataaact aaataataca ggttccggtt agcctgcgga tctctagacc
     2521 taataacttc gtatagcata cattatacga agttatatta agggttgtcg acctgcagcg
     2581 tacgaagctt cagctgcccg ggtaagccga tcccattacc gacatttggg cgctatacgt
     2641 gcatatgttc atgtatgtat ctgtatttaa aacacttttg tattattttt cctcatatat
     2701 gtgtataggt ttatacggat gatttaatta ttacttcacc accctttatt tcaggctgat
     2761 atcttagcct tgttactagt tagaaaaaga catttttgct gtcagtcact gtcaagagat
     2821 tcttttgctg gcatttcttc tagaagcaaa aagagcgatg cgtcttttcc gctgaaccgt
     2881 tccagcaaaa aagactacca acgcaatatg gattgtcaga atcatataaa agagaagcaa
     2941 ataactcctt gtcttgtatc aattgcatta taatatcttc ttgttagtgc aatatcatat
     3001 agaagtcatc gaaatagata ttaagaaaaa caaactgtac aatcaatcaa tcaatcatca
     3061 cataaaacta gtgcaaaatg gcgtattttc tggattttga cgagcgggca ctaaaggaat
     3121 ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa gaagctggtt gaagtacttg
     3181 agtcaccccg gattgaagca agtatgtagt tccatttgga agagggaatg aaagaaccaa
     3241 gacggtgact ttttttttag tgttgtgcaa ccaatatgtc gtgtgtatat catggtacag
     3301 gagaatgtca atcagctaag tgtactcaac atatttcttt gtgttttgat tgcgaacttt
     3361 gtattaccat ctcactgttg agacggctta tttgaggtaa tagctcgagt aaatgtactc
     3421 ttccatcgca aactgagcaa aaagaaagtg tgcatagcct ttgtcatact tctcctttat
     3481 tataccatga tattcagaac agtcatactg tctactcatt ttacggctat aaaaggtaac
     3541 tttcatttag attatggaaa gcactaatta tcgctgtatc aaatggttgt agagagcgca
     3601 attatgaaaa agagttacca cgtttctttt gtttcgataa aatgtccagt tgaaaacctg
     3661 ttttactaac gatttaaaaa ttgtatttca ttacaatatt ttttttgtac agacaagctc
     3721 cgtggtatgc ctgattgtta caagattaag ctccggtctt caggctatcg ccttgtatac
     3781 caggttatag acgagaaagt tgtcgttttc gtgatttctg ttgggaaaag agaacgctcg
     3841 gaagtatata gcgaggcggt caaacgcatt ctctgacctg cagggattaa tataattata
     3901 taaaaatatt atcttctttt ctttatatct agtgttatgt aaaataaatt gatgactacg
     3961 gaaagctttt ttatattgtt tctttttcat tctgagccac ttaaatttcg tgaatgttct
     4021 tataagggac ggtagattta caagtgatac aacaaaaagc aaggcgcttt ttctaataaa
     4081 aagaagaaaa gcatttaaca attgaacacc tctatatcaa cgaagaatat tactttgtct
     4141 ctaaatcctt gtaaaatgtg tacgatctct atatgggtta ctcaaggatc cacgaaaatg
     4201 atgtgaatga atacatgaaa gattcatgag atctgacaac atggtagacg tgtgtgtctc
     4261 atggaaattg atgcagttga agacatgtgc gtcacgaaaa aagaaatcaa tcctacacag
     4321 ggcttaaggg caaatgtatt catgtgtgtc acgaaaagtg atgtaactaa atacacgatt
     4381 accatggaaa ttaacgtacc ttttttgtgc gtgtattgaa atattatgac atattacaga
     4441 aagggttcgc aagtcctgtt tctatgcctt tctcttagta attcacgaaa taaacctatg
     4501 gtttacgaaa tgatccacga aaatcatgtt attatttaca tcaacatatc gcgaaaattc
     4561 atgtcatgtc cacattaaca tcattgcaga gcaacaattc attttcatag agaaatttgc
     4621 tactatcacc cactagtact accattggta cctactactt tgaattgtac taccgctggg
     4681 cgttccgcgg gccttgcgga ggataaccac caacgaactc aaatacctac cgcgtacctt
     4741 cctacggtta tgtacttttg cactgctatt gctttgagat gctatgagaa ctgccgtcaa
     4801 aattgggtgg caaagaggga tagcttcact gccaatcact acactctgtt ctgaaggagg
     4861 ggaattactg ttgtctcata aaaaaacacc tttgaaacac tttcactcat agagaagcca
     4921 agaactctga tgagttcttt atttgttgcc gctaacttta tcttatcttc tccattgtaa
     4981 taattactat tctttttact tgcctgtttt ccatcccaat gaagatccgt acatcttaga
     5041 cacccttttc tttttttctt tttttttttt tttttttttt ggcttccgaa gtgccgacaa
     5101 ctaaagaatt atcgcatttt ttttgaatac atcatactat acgatagaac gcattgaaac
     5161 ttttcccatc tcaaaatcca gggacaatag tatgggatcg atattattat caagacataa
     5221 gtattgtgat gcgcacgttg aaattcacta tttttttttt ttatatctgt ttttcaatac
     5281 agtgaacacg ataactgaaa gaaacatcta gaatgggtag cattaacctg cgtattgacg
     5341 atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag
     5401 cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc aaacagacac
     5461 tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt cgtaatccta
     5521 agccagtacg tgtgacgctg gatgaactct gacctgcagg gcgatttaat ctctaattat
     5581 tagttaaagt tttataagca tttttatgta acgaaaaata aattggttca tattattact
     5641 gcactgtcac ttaccatgga aagaccagac aagaagttgc cgacacgaca gtctgttgaa
     5701 ttggcttaag tctgggtccg cttctttaca aatttgaaga atttctctta aacgatatgt
     5761 atattctttt cgttggaaaa gatgtcttcc aaaaaaaaaa accgatgaat tagtggaacc
     5821 aaggaaaaaa aaagaggtat ccttgattaa ggaacagagc tctatatatt taataaagag
     5881 tatcatcttt caaagccttt taattctgct gtaacccgta catgcccaaa atagggggcg
     5941 ggttacacag aatatataac atcgtaggtg tctgggtgaa cagtttattc ctggcatcca
     6001 ctaaatataa tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac
     6061 caaaatattg ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac
     6121 agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc tcaatggagt gatgcaacct
     6181 gcctggagta aatgatgaca caaggcaatt gacccacgca tgtatctatc tcattttctt
     6241 acaccttcta ttaccttctg ctctctctga tttggaaaaa gctgaaaaaa aaggttgaaa
     6301 ccagttccct gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg
     6361 attgtaattc tgtaaatcta tttcttaaac ttcttaaatt ctacttttat agttagtctt
     6421 ttttttagtt ttaaaacacc aagaacttag tttcgaataa acacacataa acaaacaaaa
     6481 tggcttcttt ggttaagaaa gatatgtgta tcaagatgac tatggaaggt actgttaacg
     6541 gtcatcactt taagtgtgtt ggtgaaggtg aaggtaaacc atttgaaggt actcaagttg
     6601 aaaagattag aattactgaa ggtggtccat tgccatttgc ttatgatatt ttggctccat
     6661 gttgtatgta tggttctaag actttcatta agcacgtttc tggtatccca gactacttca
     6721 aagaatcttt tccagaaggt tttacctggg aaagaactca aatctttgaa gatggtggtt
     6781 acttgactat tcaccaagat acttcattgc aaggtaacaa ctttatcttc aaagttaacg
     6841 ttatcggtgc taattttcca gctaatggtc cagttatgca aaagaaaact gctggttggg
     6901 aaccatgtgt tgaaatgttg tatccaagag atggtgtttt gtgcggtcaa tctttgatgg
     6961 ctttgaaatg tactgatggt aatcacttga cctctcattt gagaactact tacagatcca
     7021 gaaaaccatc taacgctgtt aacatgccag aatttcattt cggtgaccac agaattgaga
     7081 ttttgaaagc tgaacaaggt aagttctacg aacaatacga atctgctgtt gctagatatt
     7141 gtgaagctgc tccatcaaaa ttgggtcatc attaatatat aactgtctag aaataaattt
     7201 tttcaaagcg gccgcattcc cagaagatta gggaggtaat taattaatag gcagatttct
     7261 agttcttata tattactata gataaggaaa ttatacgctt gtatgcagag ataatttaag
     7321 ttttgctttg ttcgccacca ttctagttat gacctttatc tatcattcta ttttttattg
     7381 gaaaggcttt acaaaaaaca aacagtaaaa attctttaag cgaagtgaaa tcgacaatga
     7441 atatgaaatt ggtaattatc ttacattagg acttttcaat atctgccggc aggatccacg
     7501 aaaatgatgt gaatgaatac atgaaagatt catgagatct gacaacatgg tagacgtgtg
     7561 tgtctcatgg aaattgatgc agttgaagac atgtgcgtca cgaaaaaaga aatcaatcct
     7621 acacagggct taagggcaaa tgtattcatg tgtgtcacga aaagtgatgt aactaaatac
     7681 acgattacca tggaaattaa cgtacctttt ttgtgcgtgt attgaaatat tatgacatat
     7741 tacagaaagg gttcgcaagt cctgtttcta tgcctttctc ttagtaattc acgaaataaa
     7801 cctatggttt acgaaatgat ccacgaaaat catgttatta tttacatcaa catatcgcga
     7861 aaattcatgt catgtccaca ttaacatcat tgcagagcaa caattcattt tcatagagaa
     7921 atttgctact atcacccact agtactacca ttggtaccta ctactttgaa ttgtactacc
     7981 gctgggcgtt taaacgcatg cggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8821 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10141 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10201 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10381 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10441 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10501 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10561 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10621 gacggccagt gcatgcgttt a
//
