LOCUS       sequence-433729-        3969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone encoding a polyprotein that is cleaved to FRET 
            glucose sensor and SLC2A4 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-Sensor_P2A_SLC2A4
FEATURES             Location/Qualifiers
     source          1..3969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(46..48,49..51,52..759)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(1657..1659,1660..1662,1663..2373)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2383..2439
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atggctgata ctcgcattgg tgtaacaatc tataagtcgg ctggtatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      241 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      541 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      661 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccg ggatcactct cggcatggac gagctatacg gtagccgaga taactttatg
      781 tctgtagtgc gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg
      841 atgaatgatt ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg
      901 aaaggggtga aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt
      961 gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag
     1021 gcgctggata gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt
     1081 attcaaggcg atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa
     1141 gacggtcaga ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca
     1201 cgtaccactt acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag
     1261 ttagataccg caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct
     1321 ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc
     1381 gcggttgaag cgctgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg
     1441 ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg cgggcaccgt actgaacgat
     1501 gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggtaaaggt
     1561 gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc
     1621 gtagataaag acaacctggc tgaattcagc aagaaaatgg tgagcaaggg cgaggagctg
     1681 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1741 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1801 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     1861 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1921 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1981 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2041 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2101 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2161 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2221 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     2281 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2341 gggatcactc tcggcatgga cgagctgtac aagggatccg gcgcaacaaa cttctctctg
     2401 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgccttctgg cttccagcag
     2461 atcggcagcg aggacggaga gccacctcag cagagggtga ccggcacact ggtgctggcc
     2521 gtgttctctg ccgtgctggg cagcctgcag tttggctaca acatcggcgt gatcaatgcc
     2581 ccacagaaag tgatcgagca gagctataac gagacctggc tgggccggca gggaccagag
     2641 ggacctagct ccatcccacc cggcacactg accacactgt gggccctgtc cgtggccatc
     2701 ttctctgtgg gcggcatgat ctctagcttt ctgatcggca tcatctccca gtggctggga
     2761 aggaagaggg caatgctggt gaacaatgtg ctggccgtgc tgggaggatc tctgatggga
     2821 ctggcaaacg cagcagcaag ctacgagatg ctgatcctgg gcaggttcct gatcggagca
     2881 tactctggac tgaccagcgg actggtgcca atgtatgtgg gcgagatcgc accaacccac
     2941 ctgaggggcg ccctgggcac actgaatcag ctggccatcg tgatcggcat cctgatcgca
     3001 caggtgctgg gactggagag cctgctggga accgcatccc tgtggcctct gctgctgggc
     3061 ctgacagtgc tgccagccct gctgcagctg gtgctgctgc cattttgccc cgagagccct
     3121 aggtacctgt atatcatcca gaatctggag ggaccagcaa ggaagtccct gaagcgcctg
     3181 accggatggg cagacgtgtc tggcgtgctg gccgagctga aggatgagaa gcgcaagctg
     3241 gagcgggaga gacccctgtc cctgctgcag ctgctgggct ctcggacaca cagacagcct
     3301 ctgatcatcg ccgtggtgct gcagctgtcc cagcagctgt ctggcatcaa cgccgtgttc
     3361 tactattcca cctctatctt tgagacagca ggagtgggac agccagcata cgcaaccatc
     3421 ggcgccggcg tggtgaatac cgtgttcaca ctggtgtctg tgctgctggt ggagagggca
     3481 ggccggagaa ccctgcacct gctgggactg gcaggaatgt gcggctgtgc catcctgatg
     3541 acagtggccc tgctgctgct ggagagagtg cctgccatga gctacgtgag catcgtggcc
     3601 atcttcggct ttgtggcctt ctttgagatc ggccctggcc caatcccctg gttcatcgtg
     3661 gccgagctgt tttcccaggg acctagacca gcagcaatgg cagtggcagg ctttagcaac
     3721 tggacctcca atttcatcat cggcatgggc tttcagtacg tggccgaggc catgggccca
     3781 tacgtgttcc tgctgtttgc cgtgctgctg ctgggcttct ttatcttcac atttctgagg
     3841 gtgccagaga ccaggggacg cacattcgat cagatcagcg ccgcctttca cagaaccccc
     3901 tccctgctgg agcaggaggt gaagccttcc acagagctgg agtatctggg cccagacgag
     3961 aacgattaa
//
