LOCUS       sequence-433735-        9746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introduction of a cyanamide-inducible I-SceI expression cassette and
            a TetR-TUP1 expression cassette, use together with pJQR3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   McDonnell L, Evans S, Lu Z, Suchoronczak M, Leighton J, Ordeniza E, 
            Ritchie B, Valado N, Walsh N, Antoney J, Wang C , Scott C, Speight 
            R, Vickers CE, Peng B
  TITLE     Cyanamide-inducible expression of homing nuclease I-SceI for 
            iterative genome engineering and parallel promoter characterisation 
            in Saccharomyces cerevisiae
  JOURNAL   bioRxiv 2024.03.26.586389
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.26.586389"
COMMENT     Sequence Label: pToxEXPr6
FEATURES             Location/Qualifiers
     source          1..9746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(343..590)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     556..574
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(598..1626)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(598..1626)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(1645..1662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1645..1661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1654..1676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1870..1889)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1989..2011
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2049..2067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2135..2239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2240..2308,2309..3100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2458..2477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3271..3859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3760..3779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4013..4030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4147..4168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4183..4188,4189..4206,4207..4213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4221..4237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4226..4248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4245..4261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4245..4261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggccctcagg gcaagccgaa ggtagccttt tttatttcgt aatcctgtag atattcttcc
       61 agcggttttg ccgttcccag agaatgaatt tcaccactgt ctttatcaat aataaaaggt
      121 gcgttaccag ctaagcgcgc ggcctcatct ccagtttcga gaaattctcg tgcttcgaaa
      181 cagaaatacc agccctggct aaagcgtcca tgtagagtaa tgacgaccgg gagatctgca
      241 tcatcaaggt aatggttcgc tttcgcgaat gcgtcgtgat aagtaatcat tctagactat
      301 attaccctgt tatccctagc gtaactttaa gggttctcga gagctcgtta aagccttcga
      361 gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct
      421 gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat
      481 aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc
      541 ggatgtgggg ggagggcgtg aatgtaagcg tgacataact aattacatga ctcgagatta
      601 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
      661 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
      721 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
      781 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
      841 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
      901 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
      961 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     1021 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     1081 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     1141 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     1201 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     1261 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     1321 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
     1381 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     1441 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     1501 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     1561 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     1621 acccatggtt gtttaaacgc atgcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1681 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1741 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1801 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     1861 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1921 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1981 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2041 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2101 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2461 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2761 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4141 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4201 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4261 ccatgattac gccgtttaaa ctgttactga tatgtctgag gaaagttgat caagacccaa
     4321 taataatcta tatactggtt gaaacaaatc agtgccggta acgctttttg tatcttgaga
     4381 tggcgtattt ctactccagc attctagtta agaaaaagtc taaaaatggg cggccgcgat
     4441 atacatgtga ttaatacaga aggtaaatgg caagacgaaa tttgcttgtt tataatacaa
     4501 aaaaacatac ataagatata atatatattt caatagaaaa gaaaaagtat agaaaagtga
     4561 ataaacaaaa gaaacatgag aaaaatagct cgtcttctca actgggtatc ctgcaggtta
     4621 atttggcgct atttttttat acttccaaat ccttgcttta caatcaccgc taccagtagc
     4681 aaaaacgtta tattctggac ccagcggaga cccgtttgcc acagccacag atataactga
     4741 attcctatga ccttgcaaca tcaataacgg attgccggat ttcttatccc aaaacaggac
     4801 accacgatct ttggaaccgg acaagatgta ctcatcattt tgtgtggtgg ccacggacaa
     4861 tacaaagtct ttatgcccga tatacgtaac ttcacaagtg ccggaatttg gagttttcga
     4921 atcgctcttg ttgtttgcat tctgcaaatt ccagagctta acagatctat ctaatgagcc
     4981 ggatacaacg ctttgtccat ctctcgtgaa gacaacgcta taaacagagt ccttgtggcc
     5041 tgtaccggat tcgttttccg aatctagtct ttccaccaag aatccggtct cggaatccca
     5101 aactctcaca gcacgatcta gagaaccagc agcgatgtat ttaccatcac ctggtgatac
     5161 agcgacggtg gtaacaccat cttcaatgga taaagtcaat gaacactggc ctgtacgtaa
     5221 gtcccaaata cgaacggtac ggtcaccaga accggagact aatttgtcac ctgagggaaa
     5281 gtagtccaat gaataaatat cttgttcgtg gccttgaaga atcataacaa tctttctatt
     5341 ttcaatatcc caaattctaa tcagtctgtc ttcagcacct gttgccaaaa atttcccatc
     5401 tggagaaaaa cacactgaac ggatatacaa gtcggatgat ggggaagacg aagtgttcag
     5461 gttttccaca tcttttgcca attctgctgc cgaagtcatc gctgtggtag taattgtggt
     5521 ggtagtagtg gttgtcattg tattgttatc cgtggacgtg gtggtgttat tttcagtgat
     5581 cgaatttcga tggttattgg cagcagaatc gtcagataaa cgggccacca gagaaccgtc
     5641 tgaaacgcga tacacttgag tagttttgtt gcagcctgtg gctaagtatt caccatcgtt
     5701 actgaacttc acgcaacaaa caactgaagt atgatccaaa gatttgtgta actcaacgtc
     5761 aatttctctt ggtagtgccg ggttgtataa aatataataa tcatttgttt gcttcttcag
     5821 agcatcggga acagactggg aatctagatc caaaaggaaa ggtgggatag gtttagagtg
     5881 gtttgctctt tgattataag ggactaagta gtgatcctgg tgcaaactag ccggggtggc
     5941 gtcttcctcc ttaggtttga tttcagtttc agttgcggtg gtagtggtgg ccgtggtggc
     6001 ggatccggtg tcatttatct tcgaggtatt attattttcc ggttcagttt ctttcaacgt
     6061 agattcaggt gccttgacag agggtaaagt agtggtctct ttagaagtag gagatccgtt
     6121 gatggctgtg ttactcaatg gtgccacgga aacctgggga ggtggttgct gttgttgaag
     6181 ttgctgctgt tgcagttgct gttgtggtag ttgttgttgg gcgtttgagg acaccacagg
     6241 caaagtggtg gtaggcaact gtgagccaac cagattagga cggctagctt gtactgggaa
     6301 ggccgatggc gaaccagtag ttgtgtttgc tgctggagtg gcggtggccg aagtagtagc
     6361 cggtggttgt tgcgcaactg gaacagatgc agatgcagcg gctagctgct gttgttgctg
     6421 ttgtaaatgc tgctggacct gttgctgctg ttgctgttgc tgctgttgct gttgctgctg
     6481 ttgctggacg gtcaaagatg caatttgatg gtctctttgc tccagcccta gtttcaagtg
     6541 cttgatctct tcttcgtacg cgtccttcat tttcctgtga gttagctcca gttcgtagac
     6601 ggtgtttctt atctgctgca tctcagccag ctgctggttc attttgaaat cgtaatcctt
     6661 ttggttttga agacggtagg tatttgcctc ttgtgagact tggagaaact cctgtctgat
     6721 ggcatcgaga agctcattca gcttattctg cgtattcgaa acgctggcag tcaactttct
     6781 cttctttctt ttagcagttg tagaggtaga ggagccagat tcacatttca actgtttctc
     6841 caaaccgcag atgatcaatt ccaaaccaaa caaaaaagct ggttcagcac cttgatgatc
     6901 aaacaactca atggcttgtc tcaataatgg tggcatagaa tcagtagttg gagtttccct
     6961 ttcttctttg gcgacttgat gttcttgatc ttccaaaaca caacccaagg taaaatgacc
     7021 aacagcagac aaagcataca aggcgttttc taaagagaaa ccttgttgac acaagaaagc
     7081 caactggttt tccaaagttt cgtattgctt ttcagttggt ctagtaccca agtgaacttt
     7141 agcaccatct ctatgagaca acaaagcaca tctgaaagac ttggcgttgt ttctcaagaa
     7201 atcttgccat gattcacctt ccaatggaca gaaatgggta tgatgtctat ccaacatttc
     7261 aatggccaaa gcgtccaaca aagctctttt gttcttaaca tgccagtaca aggtaggttg
     7321 ttcaacaccc aatttttgag ccaactttct agtggtcaaa ccttcaatac caacttcgtt
     7381 caacaattcc aaggcagagt taataacctt ggacttatcc aatctggaca tgatccgcgg
     7441 agtggcggtt gttgtcgtag gtgggggagg aggaggttgt tatgtttgac actgagttaa
     7501 agccttggaa gtttaaataa aacaagaaga caagaagaaa ggaaaatttc gctaaaaaac
     7561 atatttctgt attcatttct cgagttgtcc attgaggtga aatttcttct aatatatagt
     7621 gaatgtaggg agcgagaaca aaagagaaca aggtgttcaa aaccacgttc atcactcggt
     7681 acgatgtata tttgcgactc tttttagccc tacttaatgc tgagccactt tgccttacaa
     7741 ccctcataaa tgtattttta tcctagcttc tttttccata ttgccctagg tcttgtttat
     7801 tcatgtcacg tgactatatt tccaggtttt tctttttttc ctcgtccctt ttgtgggaaa
     7861 agaaagcgtc cttactaaaa atgagggagc tttaacttta atatacagct tttggcagct
     7921 tggtgttgtt tttcattgta ttaattaacc gctttgaaag atgatactct ttatttctag
     7981 acagttatat atatatatat atatatatat atatatatat actcgagtta ttacttaagg
     8041 aaagtttcgg aggagatagt gttcggcagt ttgtacatca tctgcgggat caggtacggt
     8101 ttgatcaggt tgtagaagat caggtaagac atagaatcga tgtagatgat cggtttgttt
     8161 ttgttgattt ttacgtaaca gttcagttgg aatttgttac gcagaccctt aaccaggtat
     8221 tctacttctt cgaaagtgaa agactgggtg ttcagtacga tcgatttgtt ggtagagttt
     8281 ttgttgtaat cccatttacc accatcatcc atgaaccagt atgccagaga catcggggtc
     8341 aggtagtttt caaccaggtt gttcgggatg gtttttttgt tgttaacgat gaacaggtta
     8401 gccagtttgt tgaaggcttg gtgtttgaaa gtctgggcgc cccaggtgat taccaggtta
     8461 cccaggtggt taacacgttc ttttttgtgc ggcggggaca gtacccactg atcgtacagc
     8521 agacatacgt ggtccatgta tgctttgttt ttccactcga actgcataca gtaggtttta
     8581 ccttcatcac gagaacggat gtaagcatca cccaggatca gaccgatacc tgcttcgaac
     8641 tgttcgatgt tcagttcgat cagctgggat ttgtattctt tcagcagttt agagttcgga
     8701 cccaggttca ttacctggtt tttttgatgc attttactag tgattgattc ttttgaagag
     8761 aagcaaggca aaaaacagta cgaagcaact tcatgttaac agaagaagaa ggaaactaac
     8821 ccatttatat ataattttat gaggggaccg atcctaaaaa gttatttgta aagctcgtac
     8881 aagattgatt gatgacttcc cgacaaacta ttgtgcggtg gagtattttg tattatagaa
     8941 atgatagtgt ccatgcactt taataagatc ttttttgcgg acatttgttt cggggccgtc
     9001 caaagttgtt tgatggtata actccgcata tttgacatct ttctctttta aaccagtatc
     9061 ggttagtctt ttgcaatttt cttgagggat tcagtctaga aagttgattt tcattgctgt
     9121 taagataaaa attccttcta acccagcttg tactccgtat actttcaatg ctctcaaaaa
     9181 gatatggata ctagttagtt tagaccctgt agtttcacca gctgctagta ttcaacgttc
     9241 atgcgacttc tataaaggag tgcttggctt tgatgttaca acattcggga actatcgcag
     9301 ggctttgtct ttttttggag tcagaaaaat caatttgcat gaggtgggaa aggaatttga
     9361 gcccaaggca cagaagccaa ctctcggatc tgtcgatctg tgttttatct tagaggatcc
     9421 tttgaataac agttacccat cttgaaagct tggattttcc aattgaagag ggcactgtaa
     9481 tccgtacagg cgcagtcaac cctattatca gtatacatcc gtgattaaga tgaaagtgtg
     9541 atggaaatat caaatagaat aaaagaaacg gaatagagct attttttttt ttatattata
     9601 gacatagtct aagcgagttt aacctttgaa atataaaaaa aaacaaaagg aaaaaagtag
     9661 tatgtgggct gcactaaact aaaaccgtga ggtatttaga tatatattct acattgtttt
     9721 acgttatagc atgtataaag agctcg
//
