LOCUS       sequence-433736-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid-free in-situ promoter cloning and characterisation system in
            S. cerevisiae, allows the characterisation of a bidirectional 
            promoter using yEGFP and E2-Crimson as reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   McDonnell L, Evans S, Lu Z, Suchoronczak M, Leighton J, Ordeniza E, 
            Ritchie B, Valado N, Walsh N, Antoney J, Wang C , Scott C, Speight 
            R, Vickers CE, Peng B
  TITLE     Cyanamide-inducible expression of homing nuclease I-SceI for 
            iterative genome engineering and parallel promoter characterisation 
            in Saccharomyces cerevisiae
  JOURNAL   bioRxiv 2024.03.26.586389
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.26.586389"
COMMENT     Sequence Label: pILGFPEasy9R
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(206..222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(219..241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(254..260,261..278,279..284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    299..320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(437..454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(608..1196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(688..707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1367..2158,2159..2227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1990..2009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2228..2332)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2400..2418
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2456..2478)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2578..2597
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2791..2813
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2805..2822
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2806..2822
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(3955..4225)
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     CDS             complement(4235..4948)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            4987..6343
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4987..5330
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5331..6140
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5398..5417)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6146..6343
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 agagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga
       61 agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata
      121 cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtatttt
      181 agcggtttaa actggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      541 gaaagaacat gtgagaaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1441 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1501 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1561 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1621 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1681 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1741 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1801 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1861 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1921 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1981 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2041 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2101 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2161 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2221 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2281 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2341 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2401 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2461 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2521 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     2581 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2641 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     2701 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2761 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2821 gtgtttaaac ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca
     2881 cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt
     2941 attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa acatgtccac
     3001 aaaatcatat accagtagag ctgagactca tgcaagtccg gttgcatcga aacttttacg
     3061 tttaatggat gaaaagaaga ccaatttgtg tgcttctctt gacgttcgtt cgactgatga
     3121 gctattgaaa cttgttgaaa cgttgggtcc atacatttgc cttttgaaaa cacacgttga
     3181 tatcttggat gatttcagtt atgagggtac tgtcgttcca ttgaaagcat tggcagagaa
     3241 atacaagttc ttgatatttg aggacagaaa attcgccgat atcggtaaca cagtcaaatt
     3301 acaatataca tcgggcgttt accgtatcgc agaatggtct gatatcacca acgcccacgg
     3361 ggttactggt gctggtattg ttgctggctt gaaacaaggt gcgcaagagg tcaccaaaga
     3421 accaagggga ttattgatgc ttgctgaatt gtcttccaag ggttctctag cacacggtga
     3481 atatactaag ggtaccgttg atattgcaaa gagtgataaa gatttcgtta ttgggttcat
     3541 tgctcagaac gatatgggag gaagagaaga agggtttgat tggctaatca tgaccccagg
     3601 tgtaggttta gacgacaaag gcgatgcatt gggtcagcag tacagaaccg tcgacgaagt
     3661 tgtaagtggt ggatcagata tcatcattgt tggcagagga cttttcgcca agggtagaga
     3721 tcctaaggtt gaaggtgaaa gatacagaaa tgctggatgg gaagcgtacc aaaagagaat
     3781 cagcgctccc cattaattat acaggaaact taatagaaca aatcacatat ttaatctaat
     3841 agccacctgc attggcacgg tgcaacacta cttcaacttc atcttacaaa aagatcacgt
     3901 gatctgttgt attgggatca ctagtaaata atatccttct cgaaagcttt aacgaacgca
     3961 gaattttcga gttattaaac ttaaaatacg ctgaacccga acatagaaat atcgaatggg
     4021 aaaaaaaaac tgcataaagg cattaaaaga ggagcgaatt tttttttaat aaaaatctta
     4081 ataatcatta aaagataaat aatagtctat atatacgtat ataaataaaa aatattcaaa
     4141 aaataaaata aactattatt ttagcgtaaa ggatggggaa agagaaaaga aaaaaattga
     4201 tctatcgatt tcaattcaat tcaatttaag atcttttgta caattcatcc ataccatggg
     4261 taataccagc agcagtaaca aattctaaca agaccatgtg gtctctcttt tcgtttggat
     4321 ctttggataa ggcagattga gtggataagt aatggttgtc tggtaacaag actggaccat
     4381 caccaattgg agtattttgt tgataatggt cagctaattg aacagaacca tcttcaatgt
     4441 tgtgtctaat tttgaagtta actttgatac cattcttttg tttgtcagcc atgatgtaaa
     4501 cattgtgaga gttatagttg tattccaatt tgtgacctaa aatgttacca tcttctttaa
     4561 aatcaatacc ttttaattcg attctattaa ctaaggtatc accttcaaac ttgacttcag
     4621 ctctggtctt gtagttaccg tcatctttga aaaaaatagt tctttcttga acataacctt
     4681 ctggcatggc agacttgaaa aagtcatgtt gtttcatatg atctgggtat ctcgcaaaac
     4741 attgaacacc ataaccgaaa gtagtgacta aggttggcca tggaactggc aatttaccag
     4801 tagtacaaat aaattttaag gtcaatttac cgtaagtagc atcaccttca ccttcaccgg
     4861 agacagaaaa tttgtgacca ttaacatcac catctaattc aaccaaaatt gggacaacac
     4921 cagtgaataa ttcttcacct ttagacattt tggatcctat attaccctgt tatccctagc
     4981 gtaactgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
     5041 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
     5101 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
     5161 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
     5221 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
     5281 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg
     5341 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     5401 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     5461 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     5521 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     5581 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag
     5641 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     5701 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     5761 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     5821 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     5881 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     5941 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     6001 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     6061 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     6121 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt
     6181 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     6241 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     6301 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     6361 acgccgccat ccagtgtcga aaacgagagt taattaatag tcttggatgt aattcttatt
     6421 gttatactga atacgctaaa accactcaca acaagtatgg agtatattgt gtctctttat
     6481 atactgagta cttatgcaat atgcgctcac tcaggatgaa atgtacacag ccgaaagtat
     6541 attgaaagct gcctctgtgg aaacttctat ctaatgttgt ctccagatgt agactatgag
     6601 gcctgaagaa gtctttaaac acctgttgga gagtataagg agactgctac aacaacgtct
     6661 tccccacaaa aattatgtgg aggccggtat gatacctgca caaacgttaa gttacacatg
     6721 aaaaagagac tgacataact ttgatctctg aaaatatgtt ttcccctgag tagcttcact
     6781 gcttggatac caatacgaat agaccttggc tatagtaagt tgcatctgta ccgtagagat
     6841 tcttgcaacc tcgcttaaac tctcgctttt atataatatt tctccttatt gcgcgcttcg
     6901 ttgaaaattt cgctaaacac ggggtttaag tttaagttta caggatttat ccggaagttt
     6961 tcgcggaccc cacacaatta agaattggct cgaagagtga taacgcatac ttttcttttc
     7021 ttttttcagt tcctagcgta cctaacgtag gtaacatgat ttggatcgtg ggatgataca
     7081 aacaacgtaa gatgagtagt tccttcctca attcttcttt cagcatcatt ttcttgaggc
     7141 gctctgggca aggtataaaa agttccatta atacgtctct aaaaaattaa accatctatc
     7201 tcttaagcag tttttttgat aatctcaaat gtacatcagt caagcgtaac taaaatacat
     7261 aagtgtaaat ggtaagccga tacgtacccg atatgggcga tctgatttgg gttgattttg
     7321 acccgacaaa aggtagcgag caagctggac atcgtccagc tgttgtcctg agtcctttca
     7381 tgtacaacaa caaaacaggt atgtgtctgt gtgttccttg tacaacgcaa tcaaaaggat
     7441 atccgttcga agttgtttta tccggtcagg aacgtgatgg cgtagcgtta gctgatcagg
     7501 taaaaagtat cgcctggcgg gcaagaggag caacgaagaa aggaacagtt gccccagagg
     7561 aattacaact cattaaagcc aaaattagta tgtagttcca tttggaagag ggaatgaaag
     7621 aaccaagacg gtgacttttt ttttagtgtt gtgcaaccaa tatgtcgtgt gtatatcatg
     7681 gtacaggaga atgtcaatca gctaagtgta ctcaacatat ttctttgtgt tttgattgcg
     7741 aactttgtat taccatctca ctgttgagac ggcttatttg aggtaatagc tcgagtaaat
     7801 gtactcttcc atcgcaaact gagcaaaaag aaagtgtgca tagcctttgt catacttctc
     7861 ctttattata ccatgatatt cagaacagtc atactgtcta ctcattttac ggctataaaa
     7921 ggtaactttc atttagatta tggaaagcac taattatcgc tgtatcaaat ggttgtagag
     7981 agcgcaatta tgaaaaagag ttaccacgtt tcttttgttt cgataaaatg tccagttgaa
     8041 aacctgtttt actaacgatt taaaaattgt atttcattac aatatttttt ttgtacagac
     8101 gtactgattg ggtaagagct catggactct accgaaaacg ttatcaaacc attcatgaga
     8161 ttcaaggtcc acatggaagg ttctgttaac ggtcatgaat ttgaaatcga aggtgtcggt
     8221 gaaggtaaac catacgaagg tactcaaact gctaagttgc aagttacaaa aggtggtcca
     8281 ttgccatttg cttgggatat tttgtctcca caattcttct atggttccaa ggcttacatc
     8341 aaacatccag ctgatattcc agactacttg aagcaatcat ttccagaggg ttttaagtgg
     8401 gaaagagtca tgaacttcga agatggtggt gttgttactg ttacccaaga ttcttcattg
     8461 caagacggta ctttgatcta ccacgttaag ttcattggtg tcaactttcc atctgatggt
     8521 ccagtcatgc aaaagaaaac tttaggttgg gaaccatcca ccgaaagaaa ttatccaaga
     8581 gatggtgttt tgaagggtga aaatcacatg gccttgaaat tgaaaggtgg tggtcattat
     8641 ttgtgcgagt tcaagtctat ctacatggct aagaaaccag ttaagttgcc aggttatcac
     8701 tacgttgatt acaagttgga tattacctct cacaacgaag attacaccgt tgtcgaacaa
     8761 tacgaaagag ctgaagctag acatcacttg ttccaatgat aagcgattta atctctaatt
     8821 attagttaaa gttttataag catttttatg taacgaaaaa taaattggtt catattatta
     8881 ctgcactgtc acttaccatg gaaagaccag acaagaagtt gccgacacga cagtctgttg
     8941 aattggctta agtctgggtc cgcttcttta caaatttgaa gaatttctct taaacgatat
     9001 gtatattctt ttcgttggaa aagatgtctt ccaaaaaaaa aaaccgatga attagtggaa
     9061 ccaaggaaaa aaaaagaggt atccttgatt aaggaacatc tagtttatac atgctataac
     9121 gtaaaacaat gtagaatata tatctaaata cctcacggtt ttagtttagt gcagcccaca
     9181 tactactttt ttccttttgt ttttttttat atttcaaagg ttaaactcgc ttagactatg
     9241 tctataatat aaaaaaaaaa atagctctat tccgtttctt ttattctatt tgatatttcc
     9301 atcacacttt catcttaatc acggatgtat actgataata gggttgactg cgcctgtacg
     9361 gattacagtg ccctcttcaa ttggaaaatc caagctttca agatgggtaa ctgttattca
     9421 aaggatcctc taagataaaa cacagatcga cagatccgag agttggcttc tgtgccttgg
     9481 gctcaaattc ctttcccacc tcatgcaaat tgatttttct gactccaaaa aaagacaaag
     9541 ccctgcgata gttcccgaat gttgtaacat caaagccaag cactccttta tagaagtcgc
     9601 atgaacgttg aatactagca gctggtgaaa ctacagggtc taaactaact agtatccata
     9661 tctttttgag agcattgaaa gtatacggag tacaagctgg gttagaagga atttttatct
     9721 taacagcaat gaaaatcaac tttctagact gaatccctca agaaaattgc aaaagactaa
     9781 ccgatactgg tttaaaagag aaagatgtca aatatgcgga gttataccat caaacaactt
     9841 tggacggccc cgaaacaaat gtccgcaaaa aagatcttat taaagtgcat ggacactatc
     9901 atttctataa tacaaaatac tccaccgcac aatagtttgt cgggaagtca tcaatcaatc
     9961 ttgtacgagc tttacaaata actttttagg atcggtcccc tcataaaatt atatataaat
    10021 gggttagttt ccttcttctt ctgttaacat gaagttgctt cgtactgttt tttgccttgc
    10081 ttctcttcaa aagaatcaat cactagtaaa atgcatcaaa aaaaccaggt aatgaacctg
    10141 ggtccgaact ctaaactgct gaaagaatac aaatcccagc tgatcgaact gaacatcgaa
    10201 cagttcgaag caggtatcgg tctgatcctg ggtgatgctt acatccgttc tcgtgatgaa
    10261 ggtaaaacct actgtatgca gttcgagtgg aaaaacaaag catacatgga ccacgtatgt
    10321 ctgctgtacg atcagtgggt actgtccccg ccgcacaaaa aagaacgtgt taaccacctg
    10381 ggtaacctgg taatcacctg gggcgcccag actttcaaac accaagcctt caacaaactg
    10441 gctaacctgt tcatcgttaa caacaaaaaa accatcccga acaacctggt tgaaaactac
    10501 ctgaccccga tgtctctggc atactggttc atggatgatg gtggtaaatg ggattacaac
    10561 aaaaactcta ccaacaaatc gatcgtactg aacacccagt ctttcacttt cgaagaagta
    10621 gaatacctgg ttaagggtct gcgtaacaaa ttccaactga actgttacgt aaaaatcaac
    10681 aaaaacaaac cgatcatcta catcgattct atgtcttacc tgatcttcta caacctgatc
    10741 aaaccgtacc tgatcccgca gatgatgtac aaactgccga acactatctc ctccgaaact
    10801 ttccttaagt aataactcga gtatatatat atatatatat atatatatat atatataact
    10861 gtctagaaat aaagagtatc atctttcaaa gccggccgc
//
